Doxorubicin-loaded micelles of reverse poly(butylene oxide)–poly(ethylene oxide)–poly(butylene oxide) block copolymers as efficient “active” chemotherapeutic agents

Cambón, Adriana; Rey‐Rico, Ana; Mistry, Dharmista; Brea, José; Loza, Mabel; Attwood, David; Barbosa, Sílvia; Alvarez‐Lorenzo, Carmen; Taboada, Pablo; Mosquera, Vı́ctor

doi:10.1016/j.ijpharm.2013.01.056

Cited by 32 publications

(32 citation statements)

References 65 publications

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“…BALB/3T3 cells, which were highly sensitive to toxic species, [40] were encapsulated and cultured in HA and HA-GO 2 hydrogels in vitro to evaluate the cytocompatibility of HA-GO NC hydrogels as well as the process of enzymatic crosslinking. The living (stained green) and dead cells (stained red) were visualized under fluorescence microscopy ( Figure 8).…”

Section: Cytotoxicitymentioning

confidence: 99%

Enzymatically cross-linked hyaluronic acid/graphene oxide nanocomposite hydrogel with pH-responsive release

Song

Xiao

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Hyaluronic acid (HA) is made up of repeating disaccharide units (β-1,4-d-glucuronic acid and β-1,3-N-acetyl-d-glucosamine) and is a major constituent of the extracellular matrix. HA and its derivatives which possess excellent biocompatibility and physiochemical properties have been studied in drug delivery and tissue engineering applications. Tyramine-based HA hydrogel with good compatibility to cell and tissue has been reported recently. However, inferior mechanical property may limit the biomedical application of the HA hydrogel. In this study, HA/graphene oxide (GO) nanocomposite (NC) hydrogel was prepared through a horseradish peroxidase catalyzed in situ cross-linking process. As compared with pure HA hydrogels, incorporation of GO to the HA matrix could significantly enhance the mechanical properties (storage moduli 1800 Pa) of the hydrogel and prolong the release of rhodamine B (RB) as the model drug from the hydrogel (33 h) as well. In addition, due to the multiple interactions between GO and RB, the NC hydrogels showed excellent pH-responsive release behavior. The release of RB from the NC hydrogel was prolonged at low pH (pH 4.0) in the presence of GO, which could be attributed to the enhanced interactions between GO and HA as well as with RB. In situ three-dimensional encapsulation of mouse embryonic fibroblasts (BALB 3T3 cells) in the NC hydrogels and cytotoxicity results indicated the cytocompatibility of both the enzymatic cross-linking process and HA/GO NC hydrogels (cell viability 90.6 ± 4.25%). The enzymatically catalyzed fabrication of NC hydrogels proved to be an easy and mild approach, and had great potential in the construction of both tissue engineering scaffolds and stimuli-responsive drug release matrices.

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Section: Cytotoxicitymentioning

confidence: 99%

Enzymatically cross-linked hyaluronic acid/graphene oxide nanocomposite hydrogel with pH-responsive release

Song

Xiao

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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“…MSNs have become attractive, promising, and novel drug carriers in drug delivery and biomedicine, more due to their unique features such as mesoporous structure, high surface area, tunable pore diameter, large pore volume, and narrow pore size distribution, preserving structural stability, acceptable toxicity, enhanced drug loading capacity, controlled release, and target drug delivery. MSNs, with honeycomb‐like structure and solid frameworks with the porous structure are suitable delivery systems for the attachment of various functional groups for targeted drug moiety to a particular site . MSNs loaded with doxorubicin and conjugated with TAT peptide increase the drug localization into the nucleus in resistant MCF‐7/ADR cancer cells and effectively reverse MDR in comparison with non‐TAT peptide .…”

Section: Novel Strategies Against Mdrmentioning

confidence: 99%

Overcoming multidrug resistance in cancer: Recent progress in nanotechnology and new horizons

Majidinia

Mirza-Aghazadeh-Attari

Rahimi

et al. 2020

IUBMB Life

125

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Multidrug resistance (MDR), defined as the ability of cancer cells to gain resistance to both conventional and novel chemotherapy agents, is an important barrier in treating malignancies. Initially, it was discovered that cellular pumps dependent on ATP were the cause of resistance to chemotherapy, and further studies have found that other mechanisms such as increased metabolism of drugs, decreased drug entry, and defective apoptotic pathways are involved in this process. MDR has been the focus of numerous initiatives and countless studies have been undertaken to better understand MDR and formulate strategies to overcome its effects. The current review highlights various nano‐drug delivery systems including polymeric/solid lipid/mesoporous silica/metal nanoparticles, dendrimers, liposomes, micelles, and nanostructured lipid carriers to overcome the mechanism of MDR. Nanoparticles are novel gateways to enhance the therapeutic efficacy of anticancer agents at the target site of action due to their tumor‐targeting abilities, which can limit the unwanted systemic effects of chemotherapy agents and also reduce drug resistance. Additionally, other innovative strategies including RNA interference as a biological process used to inhibit or silence specific gene expression, natural products as MDR modulators with little systemic toxic effects, which interfere with the functions of proteins involved in drug efflux, and physical approaches such as combination of conventional drug administration with thermal/ultrasound/photodynamic strategies are also highlighted.

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“…Cambón et al, synthesized reverse poly(butylene oxide)-poly(ethylene oxide)-poly(butylene oxide) block copolymers with potential P-gp inhibitory action in MDR cell line. Doxorubicin loaded in these polymeric micelles enhanced cell accumulation and cytotoxicity in MDR ovarian NCIADR-RES cell line over expressing P-gp (Cambón et al, 2013). Methotrexate conjugated mixed micelles of pluronic F127 and P105 suppressed tumor growth in KBv MDR cells compared to physically entraped mixed micelles due to combined effect of tumor chemosensitization by pluronic and passive targeting by micelles (Chen et al, 2013).…”

Section: Nanocarriers As Potential Drug Delivery Systems In Cancer Thmentioning

confidence: 99%

Nanodrug delivery in reversing multidrug resistance in cancer cells

et al. 2014

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Different mechanisms in cancer cells become resistant to one or more chemotherapeutics is known as multidrug resistance (MDR) which hinders chemotherapy efficacy. Potential factors for MDR includes enhanced drug detoxification, decreased drug uptake, increased intracellular nucleophiles levels, enhanced repair of drug induced DNA damage, overexpression of drug transporter such as P-glycoprotein(P-gp), multidrug resistance-associated proteins (MRP1, MRP2), and breast cancer resistance protein (BCRP). Currently nanoassemblies such as polymeric/solid lipid/inorganic/metal nanoparticles, quantum dots, dendrimers, liposomes, micelles has emerged as an innovative, effective, and promising platforms for treatment of drug resistant cancer cells. Nanocarriers have potential to improve drug therapeutic index, ability for multifunctionality, divert ABC-transporter mediated drug efflux mechanism and selective targeting to tumor cells, cancer stem cells, tumor initiating cells, or cancer microenvironment. Selective nanocarrier targeting to tumor overcomes dose-limiting side effects, lack of selectivity, tissue toxicity, limited drug access to tumor tissues, high drug doses, and emergence of multiple drug resistance with conventional or combination chemotherapy. Current review highlights various nanodrug delivery systems to overcome mechanism of MDR by neutralizing, evading, or exploiting the drug efflux pumps and those independent of drug efflux pump mechanism by silencing Bcl-2 and HIF1α gene expressions by siRNA and miRNA, modulating ceramide levels and targeting NF-κB. “Theragnostics” combining a cytotoxic agent, targeting moiety, chemosensitizing agent, and diagnostic imaging aid are highlighted as effective and innovative systems for tumor localization and overcoming MDR. Physical approaches such as combination of drug with thermal/ultrasound/photodynamic therapies to overcome MDR are focused. The review focuses on newer drug delivery systems developed to overcome MDR in cancer cell.

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Doxorubicin-loaded micelles of reverse poly(butylene oxide)–poly(ethylene oxide)–poly(butylene oxide) block copolymers as efficient “active” chemotherapeutic agents

Cited by 32 publications

References 65 publications

Enzymatically cross-linked hyaluronic acid/graphene oxide nanocomposite hydrogel with pH-responsive release

Enzymatically cross-linked hyaluronic acid/graphene oxide nanocomposite hydrogel with pH-responsive release

Overcoming multidrug resistance in cancer: Recent progress in nanotechnology and new horizons

Nanodrug delivery in reversing multidrug resistance in cancer cells

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