Doxorubicin Loaded PLGA Nanoparticle with Cationic/Anionic Polyelectrolyte Decoration: Characterization, and Its Therapeutic Potency

Tsai, Li-Hui; Yen, Chia-Hsiang; Hsieh, Hao Ying; Young, Tai‐Horng

doi:10.3390/polym13050693

Cited by 13 publications

(7 citation statements)

References 46 publications

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“…The results showed an overall increase in the drug loading content from 20 μg/mg PLGA to 70 μg/mg PLGA (Figure A). DOX is regarded as a cationic drug at pH 4–6 and carboxylate-terminated PLGA is negatively charged; therefore, DOX is preferentially entrapped into the PLGA core . Entrapment of DOX into the core was further supported by the ζ-potential (Figure B), which is nearly equivalent to bare PLGA NPs, which otherwise would have exhibited a positive change in ζ-potential if the DOX were on the surface of PLGA NPs.…”

Section: Resultsmentioning

confidence: 97%

Effect of Nanoparticle Weight on the Cellular Uptake and Drug Delivery Potential of PLGA Nanoparticles

et al. 2023

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Biodegradable and biocompatible polymeric nanoparticles (NPs) stand out as a key tool for improving drug bioavailability, reducing the inherent toxicity, and targeting the intended site. Most importantly, the ease of polymer synthesis and its derivatization to add functional properties makes them potentially ideal to fulfill the requirements for intended therapeutic applications. Among many polymers, US FDA-approved poly(l-lactic-co-glycolic) acid (PLGA) is a widely used biocompatible and biodegradable co-polymer in drug delivery and in implantable biomaterials. While many studies have been conducted using PLGA NPs as a drug delivery system, less attention has been given to understanding the effect of NP weight on cellular behaviors such as uptake. Here we discuss the synthesis of PLGA NPs with varying NP weights and their colloidal and biological properties. Following nanoprecipitation, we have synthesized PLGA NP sizes ranging from 60 to 100 nm by varying the initial PLGA feed in the system. These NPs were found to be stable for a prolonged period in colloidal conditions. We further studied cellular uptake and found that these NPs are cytocompatible; however, they are differentially uptaken by cancer and immune cells, which are greatly influenced by NPs’ weight. The drug delivery potential of these nanoparticles (NPs) was assessed using doxorubicin (DOX) as a model drug, loaded into the NP core at a concentration of 7.0 ± 0.5 wt % to study its therapeutic effects. The results showed that both concentration and treatment time are crucial factors for exhibiting therapeutic effects, as observed with DOX-NPs exhibiting a higher potency at lower concentrations. The observations revealed that DOX-NPs exhibited a higher cellular uptake of DOX compared to the free-DOX treatment group. This will allow us to reduce the recommended dose to achieve the desired effect, which otherwise required a large dose when treated with free DOX. Considering the significance of PLGA-based nanoparticle drug delivery systems, we anticipate that this study will contribute to the establishment of design considerations and guidelines for the therapeutic applications of nanoparticles.

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Section: Resultsmentioning

confidence: 97%

Effect of Nanoparticle Weight on the Cellular Uptake and Drug Delivery Potential of PLGA Nanoparticles

et al. 2023

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“…Figure c demonstrates the effect of pH 1 to 10 during a 24 h contact time on the adsorption of Doxo.HCL onto MSAC@GE-SA. Doxo.HCL had a p K a value of 8.2 due to the presence of the amine (−NH 2 ) group . For Doxo.HCL, highest adsorption occurred in neutral and basic media but at acidic pH <5, the adsorbent was positively charged, while at pH >5, it was negatively charged; the Doxo.HCL molecules may exist as positive and partially negative ions between pH = 6–8 and have a p K a value of 8.248.…”

Section: Resultsmentioning

confidence: 99%

Agro-Waste Valorization into Carbonaceous Eco-Hydrogel: A Circular Economy and Zero Waste Tactic for Doxorubicin Removal in Water/Wastewater

Devre,

Gore

2023

Langmuir

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The existing work aims to evaluate the efficiency of eco-hydrogel for adsorption of pollutants prepared from biopolymeric matrix and agricultural waste-derived biochar. An efficient and reusable adsorbent, designed from the integration of maize stalk activated carbon into a gelatin-alginate composite (MSAC@GE-SA) was explored for removal of doxorubicin hydrochloride (Doxo.HCL) from polluted water. The structural properties, presence of surface functional groups, and elemental composition were explored using XRD, SEM, BET, FTIR, and XPS techniques. The key adsorption parameters such as Doxo.HCL concentration, MSAC@GE-SA amount, solution pH, and the contact time between adsorbate and adsorbents were successfully optimized for the effective removal of Doxo.HCL (q max = 239.41 mg g −1 ). The kinetic mechanism of MSAC@GE-SA fits well with a pseudo-second-order rate model (R 2 = 0.980), followed by mono-and multilayered Langmuir and Freundlich isotherms with R 2 values 0.991 and 0.993, respectively. The recyclability of MSAC@GE-SA showed great stability without any physical damage and having sustained removal efficiency up to 10 cycles (96.32 to 55.66%).

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“…It was found that DOX/PEI complexes could continuously release DOX under a certain pH condition, and the cumulative released amount of DOX increased as the pH decreased. In another study, Tsai et al prepared PLGA NPs loaded with DOX via electrostatic interaction [ 142 ]. Then, cationic PEI and anionic polyacrylic acid (PAA) were alternately deposited on the surface of the PLGA/DOX complexes.…”

Section: Pei-based Drug Delivery Systemsmentioning

confidence: 99%

Polyethyleneimine-Based Drug Delivery Systems for Cancer Theranostics

Zhao

Zhou

2022

JFB

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With the development of nanotechnology, various types of polymer-based drug delivery systems have been designed for biomedical applications. Polymer-based drug delivery systems with desirable biocompatibility can be efficiently delivered to tumor sites with passive or targeted effects and combined with other therapeutic and imaging agents for cancer theranostics. As an effective vehicle for drug and gene delivery, polyethyleneimine (PEI) has been extensively studied due to its rich surface amines and excellent water solubility. In this work, we summarize the surface modifications of PEI to enhance biocompatibility and functionalization. Additionally, the synthesis of PEI-based nanoparticles is discussed. We further review the applications of PEI-based drug delivery systems in cancer treatment, cancer imaging, and cancer theranostics. Finally, we thoroughly consider the outlook and challenges relating to PEI-based drug delivery systems.

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Doxorubicin Loaded PLGA Nanoparticle with Cationic/Anionic Polyelectrolyte Decoration: Characterization, and Its Therapeutic Potency

Cited by 13 publications

References 46 publications

Effect of Nanoparticle Weight on the Cellular Uptake and Drug Delivery Potential of PLGA Nanoparticles

Effect of Nanoparticle Weight on the Cellular Uptake and Drug Delivery Potential of PLGA Nanoparticles

Agro-Waste Valorization into Carbonaceous Eco-Hydrogel: A Circular Economy and Zero Waste Tactic for Doxorubicin Removal in Water/Wastewater

Polyethyleneimine-Based Drug Delivery Systems for Cancer Theranostics

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