Doxycycline conjugated with polyvinylpyrrolidone-encapsulated silver nanoparticles: a polymer's malevolent touch against Escherichia coli

Silva, Heloiza F. O.; Lima, Kássio M. G.; Cardoso, Mateus Borba; Oliveira, Jessica Fernanda Affonso de; Melo, Maria Celeste Nunes de; Sant’Anna, Celso; Eugênio, Mateus; Gasparotto, Luiz H. S.

doi:10.1039/c5ra10880b

Cited by 25 publications

(26 citation statements)

References 36 publications

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“…[14][15][16][17][18] For example, the conjugation of AgNPs with Doxycycline brought about the higher bactericidal effect on E. coli than when the components are administered separately. 19 Toxicity studies are required for metal nanoparticles expected application on biomedical application. Consequently, metal nanoparticles as nanomaterials are including those in diagnostics, medical imaging, and tumor therapeutics.…”

Section: Introductionmentioning

confidence: 99%

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

Halawani¹,

Hassan²,

El-Rab³

2020

IJN

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Due to the expanded bacterial genetic tolerance to antibiotics through different mechanisms, infectious diseases of MDR bacteria are difficult for treatment. Consequently, we synthesized drug conjugated nanoparticles to dissolve this problem. Moreover, the present study aims to display the cell death status treated with cefotaxime-CS-AgNPs and also, apoptosis pathways of human RPE-1 normal cells and human MCF-7 breast cancer cells. Methods: Here, we demonstrate the possibility to synthesize AgNPs and conjugate them with cefotaxime to survey the probability of cefotaxime-CS-AgNPs as an antimicrobial agent against cefotaxime-resistant strains E. coli and MRSA. Results: TEM showed the size of AgNPs, CS-AgNPs and cefotaxime-CS-AgNPs ranged from 7.42 to 18.3 nm, 8.05-23.89 nm and 8.48-25.3 nm, respectively, with a spherical shape. The cefotaxime-CS-AgNPs enhanced the high antimicrobial properties compared to AgNPs or pure antibiotic. The MIC of Cefotaxime-CS-AgNPs ranged from 3 µg/mL to 8 µg/mL against tested E. coli and MRSA bacteria. Consequently, the highest reduction in the MIC of cefotaxime-CS-AgNPs was noted against tested strains ranging from 22% to 96%. Comparing cefotaime-CS-AgNPs to AgNPs we showed that cefotaime-CS-AgNPs have no cytotoxic effect on normal cells at even 12 µg/mL for 24 hrs. The IC50 for the AgNPs and cefotaxime-CS-AgNPs was 12 µg/mL for human RPE-1 normal cells and human MCF-7 breast cancer cell lines. The pro-apoptotic genes p53, p21, and Bax of cancer cell lines significantly upregulated followed by downregulated by anti-apoptotic gene Bcl-2 after 48 hrs at 24 µg/mL, and this concentration represents the most effective dose. Conclusion: Results enhanced the conjugating utility in old unresponsive cefotaxime to AgNPs to restore its efficiency against previous strains and demonstrated potential therapeutic applications of cefotaxime-CS-AgNPs. Moreover, this research gives remarkable insights for designing nanoscale delivery and curative systems that have a pronounced cytotoxic activity on cancer cells and are safe to normal cells.

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Section: Introductionmentioning

confidence: 99%

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

Halawani¹,

Hassan²,

El-Rab³

2020

IJN

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“…Silver nanoparticles (AgNPs) have found innumerable applications that comprise biomedicine [1,2], food storage [3], sensors [4], and (electro)catalysis [5,6]. Concerning their synthesis, AgNPs may be produced via physical [7], biological [8], or chemical methods [9][10][11], being the latter more adequate in terms of cost, simplicity, and quantity of formed AgNPs.…”

Section: Introductionmentioning

confidence: 99%

Dual Role of a Ricinoleic Acid Derivative in the Aqueous Synthesis of Silver Nanoparticles

Costa

Neto

Silva

et al. 2017

Journal of Nanomaterials

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We show that sodium 9,10-epoxy-12-hydroxytetradecanoate (SEAR), an epoxidized derivative of ricinoleic acid, simultaneously functioned as reducing and stabilizing agents in the synthesis of silver nanoparticles in alkaline aqueous medium. The advantage of using SEAR is its biodegradability and nontoxicity, which are important characteristics for mitigation of environmental impact upon discharge of nanoparticles into terrestrial and aquatic ecosystems. The SEAR concentration was found to impact considerably the size distribution of silver nanoparticles (AgNPs). A concentration below the SEAR critical micelle concentration (CMC) generated 23 nm sized AgNPs with 10 nm standard deviation, while 50 nm sized AgNPs ( = 21 nm) were obtained at a concentration above the SEAR CMC. FTIR analysis revealed that the carboxylate that constitutes the SEAR hydrophilic head binds directly to the AgNPs surface promoting stabilization in solution. Finally, AgNPs turned into Ag 2 S upon contact with wastewater samples from Wastewater Treatment Plant at Federal University of Rio Grande do Norte (UFRN), Brazil, which is an interesting result, since Ag 2 S is more environmentally friendly than pure AgNPs.

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“…The band at 1083 cm −1 and 3696 cm −1 can be assigned to Mg\ \O stretching vibration. The MCT-DC composite scaffolds presented the characteristic peaks of DC at 3388, 3000, and 2950 cm − 1 which are related to OH, N\ \H, and C\ \H stretching vibrations, respectively [37,38]. The bands at 1745 and 1451 cm −1 are attributed to the amide (N\ \H) bending and aromatic N\ \H bending vibrations, respectively.…”

Section: Resultsmentioning

confidence: 98%

“…The bands at 1745 and 1451 cm −1 are attributed to the amide (N\ \H) bending and aromatic N\ \H bending vibrations, respectively. Multiple peaks between 1400 and 1700 cm − 1 are related to aromatic C_O and C_C stretching [37,38]. Furthermore strong peak at 1557 cm −1 related to the aromatic N\ \H bending.…”

Section: Resultsmentioning

confidence: 99%

Drug release, cytocompatibility, bioactivity, and antibacterial activity of doxycycline loaded Mg-Ca-TiO2 composite scaffold

et al. 2018

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Mg-Ca-TiO 2 composite scaffold loaded with different concentrations of doxycycline were prepared by space holder method. • Mg-based scaffold containing doxycycline possesses compressive strength of 5 MPa with porosities of 65%. • Antimicrobial activity of the composite scaffold increased with increasing doxycycline concentration. • Mg-based composite scaffolds containing low concentrations of doxycycline showed good biocompatibility. • These composite scaffolds showed good biological properties and desired drug release trend for bone infection treatment.

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Doxycycline conjugated with polyvinylpyrrolidone-encapsulated silver nanoparticles: a polymer's malevolent touch against Escherichia coli

Abstract: The interaction of PVP-capped silver nanoparticles with doxycycline enhances bactericidal properties.

Cited by 25 publications

References 36 publications

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

Dual Role of a Ricinoleic Acid Derivative in the Aqueous Synthesis of Silver Nanoparticles

Drug release, cytocompatibility, bioactivity, and antibacterial activity of doxycycline loaded Mg-Ca-TiO2 composite scaffold

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