Dp116, talin, vinculin and vimentin immunoreactivities following nerve transection

Abstract

The time course of the expression of Dp116, talin, vinculin and vimentin in rat sciatic nerve was investigated after experimental transection. Dp116 was still found at 5 days after experiment in some degenerating myelinated fibers of both proximal and distal stumps. The findings are consistent with the known preservation of electrical excitability of the distal nerve in the first days after injury. Some regenerating nerve fibers into the neuroma also expressed Dp116 at 25 and 40 days after nerve transection. Talin and vinculin markedly and diffusely immunostained the neuroma. Talin in the distal stump and vimentin in both proximal and distal stumps were found decreased during the time course of the experiment. Vinculin binding increased in the distal stump, due to a real overexpression or simply to a cross-reaction to degeneration products.

Ndel1 Promotes Axon Regeneration via Intermediate Filaments

“…1). This is in contrast to Vimentin mRNAs that originate from both injured axons and glial cells [12], [14]–[17], [27].…”

“…Following sciatic nerve crush or transection, Vimentin is upregulated in both glial cells and injured axons/neurons at the mRNA and protein levels [12], [14]–[17], [27]. Using RT-PCR and Western blots, we first confirmed the upregulation of Vimentin within hours following sciatic nerve crush in rats.…”

Dystrophin Dp116: A yet to Be Investigated Product of the Duchenne Muscular Dystrophy Gene

“…In rat sciatic nerve, Dp116 was expressed in a thin rim surrounding each Schwann cell-axon unit [17], whereas, in rabbit sciatic nerve, Dp116 was found to co-localize with β-dystroglycan in the sheath around each separate Schwann cell-axon unit [18,19]. In hamster peripheral nerve, Dp116 was shown to be a component of DAPC and to be involved in the stabilization of myelin [20].…”

Dystrophin Dp116: A yet to Be Investigated Product of the Duchenne Muscular Dystrophy Gene

“…The expression of Dp116 was assessed in the peripheral nerves and spinal cord of monkeys [ 15 ], and in glia, but not neurons, of the avian parasympathetic ciliary ganglion [ 16 ]. In rat sciatic nerve, Dp116 was expressed in a thin rim surrounding each Schwann cell-axon unit [ 17 ], whereas, in rabbit sciatic nerve, Dp116 was found to co-localize with β-dystroglycan in the sheath around each separate Schwann cell-axon unit [ 18 , 19 ]. In hamster peripheral nerve, Dp116 was shown to be a component of DAPC and to be involved in the stabilization of myelin [ 20 ].…”