DR (Ia-like) antigens on human melanoma cells. Serological detection and immunochemical characterization.

Wilson, B. J.; Indiveri, Francesco; Pellegrino, M. A.; Ferrone, Soldano

doi:10.1084/jem.149.3.658

Cited by 112 publications

(21 citation statements)

References 25 publications

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“…wt determination used. The expression of Ia-like antigens on melanoma cells has been described by 2 groups of investigators (Wilson et al, 1979;Winchester et al, 1978). However, in the present study, bands corresponding to the bimolecular glycoprotein with Ia-like activity were not detected in immunoprecipitates of melanoma extracts with anti-melanoma sera.…”

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confidence: 62%

Surface antigens on human melanoma cells studied with heterologous antisera

Roberts¹

1980

Br J Cancer

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Summary.-Antisera were raised in rabbits to 6 human melanoma cell lines. The cell-surface antigens recognized by these antisera were examined using cell-surface labelling with 1251, followed by immunoprecipitation of soluble extracts of the cells and polyacrylamide-gel electrophoresis of the immunoprecipitates in the presence of sodium dodecyl sulphate (SDS). Up to 16 cell-surface antigens were recognized by these antisera, and 5 of the melanoma cell lines had a similar profile of cell-surface antigens. Digestion of labelled melanoma cells with neuraminidase before immunoprecipitation revealed that 8 of the larger antigens were sialoglycoproteins. The melanoma antisera produced haemagglutination of human erythrocytes at high dilutions, but the antigens involved could not be detected by iodination. In contrast, absorption of the melanoma sera with lymphocytes and fibroblasts revealed that these cells did contain some cell-surface glycoproteins in common with melanoma cells. The melanoma antisera contained antibodies to foetal calf serum proteins, but the amounts of these proteins on the surface of melanoma-cells were very low. Immunoprecipitation of labelled melanoma cell extracts with monospecific antiserum to 32-microglobulin produced 2 bands with mol. wts corresponding to 32-microglobulin and the HLA-determinant polypeptide chain. After absorption of melanoma antisera with cross-linked foetal calf serum, erythrocytes, lymphocytes and fibroblasts, antibodies against 10 labelled antigens remained in the absorbed antisera. However, antibodies against 8 of these antigens were still detected after absorption of the melanoma antisera with melanoma cells.

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confidence: 62%

Surface antigens on human melanoma cells studied with heterologous antisera

Roberts¹

1980

Br J Cancer

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“…Although suggestive, these results do not prove a cause-effect relationship between reduction in HLA class I antigen expression and increased susceptibility to NK cell-mediated lysis of melanoma cells IGR30 and 518A, because prolonged in vitro culturing or transfection with c-myc oncogene may modify the expression of unrelated antigenic structures which modulate susceptibility to NK cell-mediated lysis. Furthermore, the results obtained with most ofthe tumor systems investigated might not be valid for melanoma cells, because the latter express molecules, such as HLA class II antigens and intercellular adhesion molecule-1 (ICAM-1) (7,8), which may play a role in the interaction ofNK cells with target cells (5; Maio, M., and S. Ferrone, unpublished results). Therefore, to eliminate the potential interference of antigens in NK cell-mediated lysis of melanoma cells, in the present study, we have characterized the effect ofthe induction of HLA class I antigens on the susceptibility to NK cell-mediated lysis ofmelanoma cells FO-I which do not express HLA class I antigens because of a structural defect in B2m gene (9,10).…”

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confidence: 99%

Reduction in susceptibility to natural killer cell-mediated lysis of human FO-1 melanoma cells after induction of HLA class I antigen expression by transfection with B2m gene.

Maio¹,

Altomonte²,

Tatake³

et al. 1991

J. Clin. Invest.

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Induction of HLA class I antigens on cultured melanoma cells FO-1 after transfection with a human or a mouse B2m gene was associated with a statistically significant reduction in their susceptibility to natural killer (NK) cell-mediated lysis. These results indicate that the structural differences between human and mouse 62-M do not abolish the ability of the HLA class I molecular complex to modulate NK cell-mediated lysis ofmelanoma cells FO-1. The role of HLA class I antigens in the phenomenon is corroborated by the ability of anti-HLA class I MAb to enhance, although to a different extent, the susceptibility of transfected FO-1 cells to NK cell-mediated lysis. Gamma interferon (IFN-'y) and tumor necrosis factor-a (TNF-a) significantly reduced the susceptibility to NK cell-mediated lysis of transfected FO-1 cells. Surprisingly, TNF-a reduced the extent of lysis more than IFN-'y, although the latter cytokine enhanced HLA class I antigen expression more than the former one. This finding, in conjunction with a reduction in the susceptibility to NK cell-mediated lysis of untransfected FO-1 cells incubated with IFN-'y or TNF-a, suggests that the two cytokines reduce NK cell-mediated lysis of transfected cells by modulating not only the expression of HLA class I antigens, but also that of other structures. Induction of HLA class I antigens and their modulation with IFN-'y did not affect the susceptibility to lymphokine-activated killer (LAK) cell-mediated lysis of transfected FO-1 cells. Characterization of the molecular mechanism(s) underlying abnormalities in HLA class I antigen expression by melanoma cells and of the role of these molecules in the interactions of melanoma cells with various types of effector cells may suggest novel immunotherapeutic approaches to melanoma. (J. Clin. Invest. 1991. 88:282-289.)

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“…These antigens are expressed on B lymphocytes, macrophages, Langerhans cells (16), and some malignant cells (17,18). In addition, Ia antigens have also been found on a small proportion of normal T cells (19)(20)(21).…”

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Anti-Ia Reactivity in Sera from Patients with Systemic Lupus Erythematosus

Okudaira¹,

Searles²,

Ceuppens³

et al. 1982

J. Clin. Invest.

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A B S T R A C T Antileukocyte antibodies in sera from patients with systemic lupus erythematosus (SLE) were characterized by determining cross-reacting specificies with the antigens defined by OKT3, OKT4, OKT8, OKM1 and anti-Ia hybridoma antibodies (Abs). T cells were prepared by sheep erythrocyte (E) rosetting after removal of adherent cells. T cells, or non-T cells, were preincubated with SLE sera at 4°C and then with monoclonal Abs. Binding by specific monoclonal Abs was assessed by two methods: rosetting with ox erythrocytes conjugated with goat anti-mouse IgG and also in the fluorescence-activated cell sorter using fluorescein isothiocyanate-conjugated goat anti-mouse IgG. Using the rosetting method, we found that sera from SLE can block the binding of monoclonal mouse hybridoma anti-Ia Abs to T cells; the blocking of other monoclonal Abs was not consistent. Using fluorescence-activated cell sorter analysis, preincubation with SLE sera lowered the intensity of staining and total percentage of either T or non-T cells stained by monoclonal anti-Ia Abs. Blocking of anti-Ia Abs binding by SLE sera was not histocompatibility leukocyte antigen (HLA)-DR restricted and was not due to Fc receptor binding. These results indicated that antibodies in SLE sera react with structures contiguous to or identical with Ia determinants. Anti-Ia activities in SLE sera correlate with SLE disease activity. In addition, there was a significant negative correlation between anti-Ia blocking activity in the sera and the percentage of Iapositive T cells in the blood of SLE patients. Antibodies in SLE sera with anti-Ia blocking activity may play Dr. Jan L. Ceuppens was supported by a fellowship from the

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DR (Ia-like) antigens on human melanoma cells. Serological detection and immunochemical characterization.

Cited by 112 publications

References 25 publications

Surface antigens on human melanoma cells studied with heterologous antisera

Surface antigens on human melanoma cells studied with heterologous antisera

Reduction in susceptibility to natural killer cell-mediated lysis of human FO-1 melanoma cells after induction of HLA class I antigen expression by transfection with B2m gene.

Anti-Ia Reactivity in Sera from Patients with Systemic Lupus Erythematosus

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