DR.lyp/lyp bone marrow maintains lymphopenia and promotes diabetes in lyp/lyp but not in +/+ recipient DR.lyp BB rats

Increased GABAA channel subunits expression in CD8+ but not in CD4+ T cells in BB rats developing diabetes compared to their congenic littermates

“…Animals. The congenic BB rats used in this study were developed as described (Hawkins et al, 2005). All animals were housed and bred in specific pathogen free conditions.…”

“…The diabetic prone (DR lyp/lyp ) rats shares characteristics such as hyperglycemia, hypoinsulinemia and weight loss with human type 1 diabetes (Elder and Maclaren, 1983;Hawkins et al, 2005). A unique feature of the BB rat is that lymphophenia is a prerequisite for diabetes (Markholst et al, 1991) in addition to cells of the immune system that are thought to be actively involved in the destruction of the pancreatic islet  cells (Ramanathan and Poussier, 2001).…”

Introgression of F344 Rat Genomic DNA on BB Rat Chromosome 4 Generates Diabetes-Resistant Lymphopenic BB Rats

“…Blood lymphocyte phenotyping. Between 25 and 30 days of age, two drops of tail vein blood were obtained and diluted in 5 ml Gey's solution as described in detail by Hawkins et al (24). The fraction of fluorescent T-cell receptor (TCR)-positive T-cells among mononuclear cells was determined on an EPICS Elite Flow Cytometer (Beckman Coulter, Fullerton, CA).…”

“…Genotyping Chromosome 4. Between 25 and 30 days of age, 5-mm tail snips were obtained and DNA isolated using a phenolchloroform extraction protocol as described in detail elsewhere (24). The samples were diluted to 25 ng/l in a 10 mmol/l Tris, 0.5 mmol/l EDTA (TE) solution and 2 l of this genomic DNA solution was used in each of the following 10-l reactions.…”

The lymphocyte guard-IANs: regulation of lymphocyte survival by IAN/GIMAP family proteins

“…IAN5 deficiency in rats also affects other lymphoid cells including natural killer T (NKT) cells in the spleen [50], and gut gd T cells and NK cells [51,52]. Other studies suggested that IAN5 affects the microenvironment wherein lymphocytes develop [53,54].…”