Dr. Novel missense variant L46Q of fatty acid synthase gene and fatty acids content in Awassi sheep

Al‐Shuhaib, Mohammed Baqur S.; Kareem, Zahraa Aqeel

doi:10.4025/actascianimsci.v44i1.56273

Cited by 13 publications

(6 citation statements)

References 29 publications

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“…The Awassi sheep is a prevalent breed throughout most Middle Eastern countries. 41 Although this breed is known for its ability to cope with unfavorable conditions, 42 its reproduction is lower than other breeds in the region, such as Karakuls and Assafs. 3 Most Middle Eastern breeders are concerned about the low reproductive capacity of this breed, which drives efforts to improve its reproductive performance.…”

Section: Discussionmentioning

confidence: 99%

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

Al‐Shuhaib

Khazaal

2023

Bioinform Biol Insights

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Several genes influence sheep’s reproductive performance, among them the paired-like homeodomain transcription factor 2 ( PITX2) gene. Thus, this study aimed to examine whether the variability within the PITX2 gene is associated with the reproductive performance of Awassi ewes. A total of 123 single-progeny ewes and 109 twin ewes were used to extract genomic DNA. An amplicon of 4 sequence fragments from exons 2, 4, 5 (upstream portion), and 5 (downstream portion) of the PITX2 gene was generated by polymerase chain reaction (PCR), 228, 304, 381, and 382 bp, respectively. Three genotypes of 382 bp amplicons were identified: CC, CT, and TT. Sequence analysis revealed a novel mutation in the CT genotype 319C > T. Statistical analysis revealed that single-nucleotide polymorphism (SNP) 319C > T was associated with reproductive performance. Single-nucleotide polymorphism 319C > T-carrying ewes had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing than those carrying CT and CC genotypes. Based on a logistic regression analysis, it was confirmed that the 319C > T SNP decreased litter size. Ewes with TT genotype produced fewer lambs than ewes with CT and CC genotypes. According to these results, the variant 319C> T SNP negatively affects the reproductive performance of Awassi sheep. Ewes carrying the 319C > T SNP have a lower litter size and are less prolific than those without the SNP.

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Section: Discussionmentioning

confidence: 99%

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

Al‐Shuhaib

Khazaal

2023

Bioinform Biol Insights

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“…Reproductive traits play a significant role in sheep productivity. 1 , 2 Genetics and the environment influence reproductive traits. Sheep reproductive traits are regulated predominantly by genetic factors.…”

Section: Introductionmentioning

confidence: 99%

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

Albazi

Al‐Shuhaib

Merzah

et al. 2023

Bioinform Biol Insights

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Reproductive traits are affected by many factors, including ovarian function, hormones, and genetics. Genetic polymorphisms of candidate genes are associated with reproductive traits. Several candidate genes are associated with economic traits, including the follistatin ( FST) gene. Thus, this study aimed to evaluate whether the genetic variations in the FST gene are associated with the reproductive traits in Awassi ewes. The genomic DNA was extracted from 109 twin ewes and 123 single-progeny ewes. Therefore, 4 sequence fragments from the FST gene were amplified using polymerase chain reaction (PCR) (exon 2/240, exon 3/268, exon 4/254, and exon 5/266 bp, respectively). For a 254 bp amplicon, 3 genotypes were identified: CC, CG, and GG. Sequencing revealed a novel mutation in CG genotypes c.100C > G. The statistical analysis of c.100C > G showed an association with reproductive characteristics. Ewes carrying the c.100C > G had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing compared with CG and CC genotypes. Logistic regression analysis confirmed that the c.100C > G single-nucleotide polymorphism (SNP) is responsible for decreasing litter size. According to these results, the variant c.100C > G negatively affects the traits of interest and is associated with lower reproductive traits in Awassi sheep. As a result of this study, ewes carrying the c.100C > G SNP have lower litter size and are less prolific.

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“…The fatty acid synthase (FASN) gene is a potential candidate for regulating livestock meat fat composition (Kaplanová et al 2013;Al-Thuwaini & Kareem 2022). The fatty acid composition of livestock meat impacts consumer health (Grzes et al 2016;Dervishi et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Synthase Polymorphism of Awassi Sheep and its Impact on Fatty Acid Composition

Merzah,

Mohammed,

Rhadi

et al. 2023

JITV

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Livestock intramuscular fat content is determined by fatty acid synthase (FASN).Â The fatty acid profile of the fat is of relevance to human health. Â Thus, this study explores the relationship between the polymorphism of the FASN gene in Awassi sheep and its impact on fatty acid composition.Â The study used 100 Awassi rams, ranging in age from 1 to 2.5 years.Â Molecular DNA was isolated from each blood sample; genotyping, sequencing reactions, and in silico tools were subsequently used to confirm the variants in amplified fragments. Â The results revealed two genotypes (GG and GA) of the ovine FASN gene (exon 2).Â The novel missense c.186 GA was identified in the genotype GA. Â The GA genotype had significantly (P0.05) increased intramuscular fat, higher unsaturated fatty acid content, and lower saturated fatty acid content than the GG genotype.Â Cumulative in silico analysis indicated a detrimental effect of the SNP c.186 GA on FASN activity.Â The intramuscular GA genotype of Awassi sheep had a low saturated content relative to unsaturated fats.Â The result confirmed that the c.186 GA SNP in ovine FASN gene variation is potentially helpful in assessing carcass traits, and this might lead to a more efficient selection of animals with healthier fatty acid profiles, resulting in higher-quality meat.

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Dr. Novel missense variant L46Q of fatty acid synthase gene and fatty acids content in Awassi sheep

Cited by 13 publications

References 29 publications

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

Fatty Acid Synthase Polymorphism of Awassi Sheep and its Impact on Fatty Acid Composition

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