Objective
The objectives of the current experiments were to develop and characterize primary rat nasal epithelial (RNE) cultures and evaluate their usefulness as a model of sinonasal transepithelial transport and CFTR function.
Study Design
Laboratory in vitro and animal studies.
Methods
CFTR+/+ and CFTR−/− rat nasal septal epithelia (RNSE) were cultured on semipermeable supports at an air-liquid interface (ALI) to confluence and full differentiation. Monolayers were mounted in Ussing chambers for pharmacologic manipulation of ion transport and compared to similar filters containing murine (MNSE) and human (HSNE) epithelia. Histology and scanning electron microscopy (SEM) were completed. Real-time PCR (RT-PCR) of CFTR+/+ RNSE, MNSE, and HSNE was performed to evaluate relative CFTR gene expression.
Results
Forskolin-stimulated anion transport (ΔIsc in μA/cm2) was significantly greater in epithelia derived from CFTR+/+ when compared to CFTR−/− animals (100.9+/−3.7 vs. 10.5+/−0.9, p<0.0001). Amiloride-sensitive ISC was equivalent (−42.3 ± 2.8 vs. −46.1 ± 2.3 p=0.524). No inhibition of CFTR-mediated Cl− secretion was exhibited in CFTR−/− epithelia with the addition of the specific CFTR inhibitor, CFTRInh-172. However, calcium-activated Cl− secretion (UTP) was significantly increased in CFTR−/− RNSE (CFTR−/− −106.8 ± 1.6 vs. CFTR+/+ −32.2 ± 3.1;p<0.0001). All responses were larger in RNSE when compared to CFTR+/+ and CFTR−/− (or F508del/F508del) murine and human cells (p<0.0001). SEM demonstrated 80–90% ciliation in all RNSE cultures. There was no evidence of infection in CFTR−/− rats at 4 months. CFTR expression was similar among species.
Conclusion
The successful development of the CFTR−/− rat enables improved evaluation of CF sinus disease based on characteristic abnormalities of ion transport.