Draft Genome of a Brazilian Avian-Pathogenic Escherichia coli Strain and In Silico Characterization of Virulence-Related Genes

Abstract

c Avian-pathogenic Escherichia coli (APEC) strains cause extraintestinal diseases in avian species. Here, we present the draft genome of an APEC strain (SCI-07) from Brazil that was isolated from skin lesions (gelatinous edema) on the head and periorbital tissues of a laying hen with swollen head syndrome.

Complete Genome Sequence of the Avian Pathogenic Escherichia coli Strain APEC O78

“…The sequence of APEC O1, an O1:K1:H7 strain isolated from the lung of a turkey, is fully closed (9). Obtained from gelatinous edema lesions from a laying hen, a draft sequence of a Brazilian APEC strain, SCI-07, a member of the O nontypeable:H31 serotype, is in 68 contigs (10), and a sequence of an O78 strain (χ7122) was recently released in 12 contigs (11). Here, we describe a fully closed and annotated sequence of an O78 strain with the idea that fully closed sequences representative of the most commonly isolated APEC serogroups, such as O1 and O78 strains, are needed to adequately support future colibacillosis research (1).…”

In silico phylogenetic and virulence gene profile analyses of avian pathogenic Escherichia coli genome sequences

“…This suggests that the core genome of ST23 strains has the potential to generate variants able to cause disease on avian or human, depending of the accessory genome. For urinary tract infections and new-born meningitis, it has been hypothesized that APEC could access and colonize the human colon via the ingestion of contaminated poultry and then reach the urinary tract or the central nervous system (Rodriguez-Siek et al The availability of APEC genome sequences (Johnson et al 2007, Rojas et al 2012 permits the comparison of genome contents between APEC and human ExPEC strains, and helps to understand the evolutionary processes involved in shaping the phenotypes of different E. coli pathotypes. The phylogeny (based on MLST) and the cluster (based on the presence or absence of virulence genes) analyses, may be helpful to highlight the similarities between these different strains, and the potential nonspecific host relationships.…”

Genome Sequences of Avian Pathogenic Escherichia coli Strains Isolated from Brazilian Commercial Poultry

“…The availability of several APEC genome sequences (9, 10, 11) permits the comparison of genome contents between APEC and other pathogenic and nonpathogenic E. coli strains, which might help us understand the evolutionary processes involved in the shaping of the phenotypes of different pathotypes. A detailed study of these genomes and those of other available E. coli strains will be reported soon.…”