Draft genome of Kocuria polaris CMS 76orT isolated from cyanobacterial mats, McMurdo Dry Valley, Antarctica: an insight into CspA family of proteins from Kocuria polaris CMS 76orT

Abstract

Kocuria polaris strain CMS 76or(T) is a gram-positive, orange-pigmented bacterium isolated from a cyanobacterial mat sample from a pond located in McMurdo Dry Valley, Antarctica. It is psychrotolerant, orange pigmented, hydrolyses starch and Tween 80 and reduces nitrate. We report the 3.78-Mb genome of K. polaris strain CMS 76or(T), containing 3416 coding sequences, including one each for 5S rRNA, 23S rRNA, 16S rRNA and 47 tRNA genes, and the G+C content of DNA is 72.8%. An investigation of Csp family of proteins from K. polaris strain CMS 76or(T) indicated that it contains three different proteins of CspA (peg.319, peg.2255 and 2832) and the length varied from 67 to 69 amino acids. The three different proteins contain all the signature amino acids and two RNA binding regions that are characteristic of CspA proteins. Further, the CspA from K. polaris strain CMS 76or(T) was different from CspA of four other species of the genus Kocuria, Cryobacterium roopkundense and E. coli indirectly suggesting the role of CspA of K. polaris strain CMS 76or(T) in psychrotolerant growth of the bacterium.

Phylogenetic and pangenomic analyses of members of the family <i>Micrococcaceae</i> related to a plant-growth-promoting rhizobacterium isolated from the rhizosphere of potato (<i>Solanum tuberosum</i> L.)

“…The other members of this cluster include K. rosea strain DSM 20447 T , a member of the Actinobacteria; K. polaris type strain CMS 76or T , isolated from cyanobacterial mats in McMurdo Dry Valley, Antarctica (Gundlapally et al, 2015); and K. salina strain CV6 29, isolated in the vicinity of Lake Schott el Djerid (Tunisia) from the roots of Cistanche violacea, a desert plant of the Orobanchaceae family that lives on the roots of host plants (tamarix, black saxaul). The adaptation of these strains to such a contrasting diversity of habitats and conditions can be attributed to the phenotypic traits encoded by the genes in the accessory and strain-specific parts of the Kocuria pangenome, to which a large contribution is probably made by HGT (Treangen, Rocha, 2011;Koonin, 2012).…”

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

“…Most of the species isolated from media reproducing the lichen environment are known to evolve in water reservoirs and polluted environments. For instance, Aquaspirillum arcticum, Erythrobacter sp, Klenkia taihuensis, Paracoccus chinensis, Microterricola gilva, and Kocuria polaris (Table 1), were previously isolated from marine environments [39][40][41][42], sediments [43,44], seaweed [45] or cyanobacteria [46]. It is to note that various Erythrobacter, Paracoccus, Kocuria, Pseudomonas or Gordonia species were already isolated from marine or maritime Brittany lichens and P. helmanticensis from an island Austrian lichen [42].…”

Rhizocarpon geographicum Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance

“…Most of the species isolated from the media reproducing the lichen environment were previously reported from water reservoirs and polluted environments. For instance, Aquaspirillum arcticum, Erythrobacter sp., Klenkia taihuensis, Paracoccus chinensis, Microterricola gilva, and Kocuria polaris (Table 1) were previously isolated from marine environments [39][40][41][42], sediments [43,44], seaweed [45], or cyanobacteria [46]. It is important to note that various Erythrobacter, Paracoccus, Kocuria, Pseudomonas, or Gordonia species were isolated earlier from marine or maritime Brittany lichens.…”