Draft Genome Sequence for
            <i>Caulobacter</i>
            sp. Strain OR37, a Bacterium Tolerant to Heavy Metals

Utturkar, Sagar M.; Bollmann, Annette; Brzoska, Ryann M.; Klingeman, Dawn M.; Epstein, Slava E.; Palumbo, Anthony V.; Brown, Steven D.

doi:10.1128/genomea.00322-13

Cited by 12 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although uzcR and uzcS are conserved throughout much of alpha-proteobacteria, the insertion of parDE 3 between uzcR and uzcS is unique to a subset of the Caulobacter genus; uzcR and uzcS are adjacently located in the majority of closely related alphaproteobacteria ( Fig. S1) including C. crescentus OR37, an environmental isolate from a U-contaminated site (Utturkar et al, 2013). The fact that a promoter was mapped upstream of parD 3 ( Fig.…”

Section: The Uzcrs Tcs Is a Direct Activator Of P Urcamentioning

confidence: 99%

Identification of a U/Zn/Cu responsive global regulatory two‐component system in Caulobacter crescentus

Park

Overton

Liou

et al. 2017

Molecular Microbiology

View full text Add to dashboard Cite

Despite the well-known toxicity of uranium (U) to bacteria, little is known about how cells sense and respond to U. The recent finding of a U-specific stress response in Caulobacter crescentus has provided a foundation for studying the mechanisms of U- perception in bacteria. To gain insight into this process, we used a forward genetic screen to identify the regulatory components governing expression of the urcA promoter (P ) that is strongly induced by U. This approach unearthed a previously uncharacterized two-component system, named UzcRS, which is responsible for U-dependent activation of P . UzcRS is also highly responsive to zinc and copper, revealing a broader specificity than previously thought. Using ChIP-seq, we found that UzcR binds extensively throughout the genome in a metal-dependent manner and recognizes a noncanonical DNA-binding site. Coupling the genome-wide occupancy data with RNA-seq analysis revealed that UzcR is a global regulator of transcription, predominately activating genes encoding proteins that are localized to the cell envelope; these include metallopeptidases, multidrug-resistant efflux (MDR) pumps, TonB-dependent receptors and many proteins of unknown function. Collectively, our data suggest that UzcRS couples the perception of U, Zn and Cu with a novel extracytoplasmic stress response.

show abstract

Section: The Uzcrs Tcs Is a Direct Activator Of P Urcamentioning

confidence: 99%

Identification of a U/Zn/Cu responsive global regulatory two‐component system in Caulobacter crescentus

Park

Overton

Liou

et al. 2017

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…C. crescentus is an aquatic, aerobic bacterium that is able to survive under low-nutrient conditions (23). Caulobacter species are able to tolerate high concentrations of U(VI) (24) and have been found in U-contaminated sites (25). Exposure of C. crescentus to U(VI) has been shown to elicit U-specific cellular responses on both the transcriptional and proteomic levels (24,26,27).…”

mentioning

confidence: 99%

Biomineralization of Uranium by PhoY Phosphatase Activity Aids Cell Survival in Caulobacter crescentus

Yung

Jiao

2014

Appl Environ Microbiol

View full text Add to dashboard Cite

Caulobacter crescentus is known to tolerate high levels of uranium [U(VI)], but its detoxification mechanism is poorly understood. Here we show that C. crescentus is able to facilitate U(VI) biomineralization through the formation of U-P i precipitates via its native alkaline phosphatase activity. The U-P i precipitates, deposited on the cell surface in the form of meta-autunite structures, have a lower U/P i ratio than do chemically produced precipitates. The enzyme that is responsible for the phosphatase activity and thus the biomineralization process is identified as PhoY, a periplasmic alkaline phosphatase with broad substrate specificity. Furthermore, PhoY is shown to confer a survival advantage on C. crescentus toward U(VI) under both growth and nongrowth conditions. Results obtained in this study thus highlight U(VI) biomineralization as a resistance mechanism in microbes, which not only improves our understanding of bacterium-mineral interactions but also aids in defining potential ecological niches for metal-resistant bacteria.

show abstract

“…are found in nearly all aquatic environments (Poindexter, ), including those that are nutrient‐poor (Poindexter, ) or characterized by high heavy metal content (e.g. uranium (Utturkar et al ., )). While the molecular networks that control cell cycle progression in C. crescentus have been well studied (Curtis and Brun, ) less is known about the signal transduction pathways that enable a sense and respond functionality in the diverse environments that C. crescentus inhabits.…”

Section: Introductionmentioning

confidence: 99%

The UzcRS two‐component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators

Park

Overton

Jiao

2019

Molecular Microbiology

View full text Add to dashboard Cite

Summary The UzcRS two‐component system in Caulobacter crescentus mediates widespread transcriptional activation in response to the metals U, Zn and Cu. Unexpectedly, a screen for mutations that affected the activity of the UzcR‐regulated urcA promoter (PurcA) revealed four previously uncharacterized proteins whose inactivation led to metal‐independent induction of PurcA. Using molecular genetics and functional genomics, we find that these auxiliary regulators control PurcA expression by modulating the activity of UzcRS through distinct mechanisms. An ABC transporter with a periplasmic metallo‐aminopeptidase domain forms a sensory complex with UzcRS, antagonizing metal dependent stimulation by virtue of its ATPase and peptidase domains. Two MarR‐like transcription factors synergistically regulate UzcRS activity by repressing the expression of the membrane proteins UzcY and UzcZ, which stimulate UzcRS activity and enhance its sensitivity to a more environmentally relevant U/Zn/Cu concentration range. Additionally, the membrane protein UzcX, whose expression is positively regulated by UzcR, provides a mechanism of feedback inhibition within the UzcRS circuit. Collectively, these data suggest that UzcRS functions as the core‐signaling unit within a multicomponent signal transduction pathway that includes a diverse set of auxiliary regulators, providing further insight into the complexity of signaling networks.

show abstract

Draft Genome Sequence for Caulobacter sp. Strain OR37, a Bacterium Tolerant to Heavy Metals

Cited by 12 publications

References 18 publications

Identification of a U/Zn/Cu responsive global regulatory two‐component system in Caulobacter crescentus

Identification of a U/Zn/Cu responsive global regulatory two‐component system in Caulobacter crescentus

Biomineralization of Uranium by PhoY Phosphatase Activity Aids Cell Survival in Caulobacter crescentus

The UzcRS two‐component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators

Contact Info

Product

Resources

About