Draft Genome Sequence of a Propanotroph, Rhodococcus sp. Strain ENV425, Capable of Degrading Methyl tert -Butyl Ether and N -Nitrosodimethylamine

Abstract

In this study, the draft genome of Rhodococcus sp. strain ENV425 was determined. The propane-grown strain ENV425 cometabolically degrades environmental contaminants such as methyl tert-butyl ether and N-nitrosodimethylamine. The sequence revealed the presence of multiple hydrocarbon metabolic genes that could play pivotal roles in the biodegradation of pollutants.

Biotransformation of PFAA Precursors by Oxygenase-Expressing Bacteria in AFFF-Impacted Groundwater and in Pure-Compound Studies with 6:2 FTS and EtFOSE

“…While it is likely that the inoculated cultures were responsible for the observed transformation reactions (i.e., the reproducible results in the inoculated groundwater and pure-compound studies especially for ENV494M and ENV425), the possibility that native organisms in the groundwater with the same metabolic activities (e.g., native methanotrophs in the case of ENV494M) contributed to or were responsible for the observed transformations cannot be ruled out based upon the experimental controls. Of the inoculated cultures, known oxygenases included methane monooxygenase, ammonia monooxygenase, propane monooxygenase, and short-chain alkane monooxygenase (ENV494M, M. hirsuta, AOC, ENV425, and ENV493; Hatzinger, unpublished data and ,,,,, and toluene-2-monooxygenase (B. cepacia G4; 32)).…”

“…Cultures used in studies included Rhodococcus ruber ENV425 (pure propanotroph with short-chain alkane and propane monooxygenases;), ENV408 (consortium; pentane oxidizers with presumed alkane monooxygenase(s)), Rhodococcus aetheriovorans ENV493 (pure isobutane oxidizer with short-chain alkane monooxygenase and propane monooxygenases), ENV494M (consortium; methanotrophs with methane monooxygenases), Methylocystis hirsuta CSC1 ATCC BAA 1344 (pure methanotroph with soluble and particulate methane monooxygenases), Burkholderia cepacia G4 (pure toluene oxidizer, previously called Pseudomonas cepacia G4 with toluene-2-monooxygenase), Rhodococcus erythropolis ENV407A (pure octane oxidizer, presumably with alkane oxygenase(s)), and AOC (consortium; ammonia oxidizers with ammonia monooxygenase − ). With the exception of AOC, B.…”