Draft Genome Sequence of a Biosurfactant-Producing Bacillus subtilis UMX-103 Isolated from Hydrocarbon-Contaminated Soil in Terengganu, Malaysia

Singh

2021

Preprint

Background: Bioremediation is crucial for recuperate polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Bioremediation is crucial for recuperate polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present investigation, a biosurfactant producing bacterium Bacillus tequilensis was isolated from Chilika lake, Odisha, India (latitude and longitude: 19.8450 N 85.4788 E). Whole Genome Sequencing (WGS) of Bacillus tequilensis was carried out using Illumina NextSeq 500.Results: The whole genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415, and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study, a pathway was constructed which describe the biosurfactant metabolism of Bacillus tequilensis. The study identified genes such as SrfAD, SrfAC, SrfAA, SrfAB which are involved in biosurfactant synthesis. Conclusion: The sequence of the genes SrfAD, SrfAC, SrfAA, SrfAB was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.

Section: 6metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

Whole Genome Sequence of Biosurfactant Producing Bacillus Tequilensis

Singh

2021

Preprint

“…The genes, rmlA, rmlB, rmlC and rmlD are only present in UMX − 103 strains whereas, sigA, DnaK and LytR are present speci cally in Bacillus strain. Besides, the genes comA, comP, rpoN, abrB and ResD are presented in both UMX-103 and B. subtilis 168 [18]. Based on the above literature biological annotation, we have identi ed DnaKandLytRgenes from Bacillus tequilensis and the sequence were deposited in NCBI with accession MUF99480.1 and MUG01692.1 respectively.…”

Section: Metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

“…In this study, we have identi ed biosurfactant producing genes and corresponding orfs of Bacillus tequilensis such gene SrfAD, SrfAC, SrfAA and the sequence of the same was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1,MUG03515.1 respectively. 3. srfA [16] 4. rhlB [16] 5. cfp [17] 6. srfAA [18] 7. srfB [18] 8. srfAB [18] 9. srfAD [18] 10. Spf [18] 11. ppsB [18] 12. ppsC [18] 13. ppsD [18] 14.…”

Section: Metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

“…3. srfA [16] 4. rhlB [16] 5. cfp [17] 6. srfAA [18] 7. srfB [18] 8. srfAB [18] 9. srfAD [18] 10. Spf [18] 11. ppsB [18] 12. ppsC [18] 13. ppsD [18] 14. X ppsE [18] 15.…”

Section: Metabolism Of Biosurfactant Producing Genesunclassified

“…X sigA [18] 28. abrB [18] 29. X DnaK [18] 30. LytR [18] 2.7 Biosurfactant / Lipopeptide metabolism of Bacillus tequilensis…”

Section: Metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

See 2 more Smart Citations

Whole Genome Sequence of biosurfactant producing Bacillus tequilensis

2020

Preprint

We announce the complete genome sequence of Bacillus tequilensis, a biosurfactant producing bacterium isolated from Chilika lake, Odisha, India(latitude and longitude: 19.8450 N 85.4788 E). The genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415 and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study we constructed a pathway which describe the biosurfactant metabolism of Bacillus tequilensis and identified the genes such as SrfAD, SrfAC, SrfAA which are involved in biosurfactant synthesis. The sequence of the same was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.

Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis

2023

PLoS ONE

Bioremediation is crucial for recuperating polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant-producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present investigation, a biosurfactant-producing bacterium Bacillus tequilensis was isolated from Chilika Lake, Odisha, India (latitude and longitude: 19.8450 N 85.4788 E). Whole-Genome Sequencing (WGS) of Bacillus tequilensis was carried out using Illumina NextSeq 500. The size of the whole genome of Bacillus tequilensis was 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes (ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total raw reads were 4209415, and the processed reads were 4058238 with 4492 genes. The whole genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation, and comparative genome analysis with other existing Bacillus species. In this study, a pathway was constructed which describes the biosurfactant metabolism of Bacillus tequilensis. The study identified that genes such as SrfAD, SrfAC, SrfAA and SrfAB are involved in biosurfactant synthesis. The sequence of the genes SrfAD, SrfAC, SrfAA, SrfAB was deposited in GenBank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole genome sequence was submitted to GenBank with an accession RMVO00000000 and the raw fastq reads were submitted to SRA, NCBI repository with an accession: SRX5023292.