Draft Genome Sequence of a Medium- and Long-Chain n -Alkane-Degrading Bacterium, Tsukamurella tyrosinosolvens Strain PS2, with Two Genetic Systems for Alkane Degradation

Abstract

Here, we report the genome sequence of Tsukamurella tyrosinosolvens strain PS2, which was isolated from hydrocarbon sludge of an organic synthesis factory. This strain was able to utilize a wide range of n-alkanes, from C16 to C35, as sole carbon sources.

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

“…It was early reported that strains of this genus were opportunistic pathogens (Bouza et al 2009), but recent studies have shown that human infections with Tsukamurella are seldom because the genus is a kind of saprophyte bacterium (Safaei et al 2017). The functions of the Tsukamurella are diverse, especially T. tyrosinosolvens is extensively researched, this strain was able to use alkanes as carbon sources (Romanova et al 2019), produce the biosurfactants of oligosaccharide lipids (Vollbrecht et al 1998), oxidize CO (Park et al 2009), degrade the dibutyl phthalate and hexadecane (Ogawa et al 2009), and produce lipase to resolve ethyl 2-(2-oxopyrrolidin-1-yl) butyrate (Huang et al 2016). The above showed that T. tyrosinosolvens is mostly related to the degradation and metabolism of xenobiotics, but the impact on plants is only seen in Sang's report ( 2013), which T. tyrosinosolvens isolated from pepper roots could against Phytophthora capsici and reduce anthracnose occurrence under the field conditions.…”

Obligate aerobic, gram-positive, weak acid-fast, nonmotile bacilli, Tsukamurella tyrosinosolvens: Minireview of a rare opportunistic pathogen

“…This indicates that further studies of groEL gene sequencing of multiple strains of each Tsukamurella species would be merited, as verification of suitability for speciation of Tsukamurella [ 1 , 11 ]. Furthermore, regarding the biodegradation of hydrocarbons, two alkane catabolic pathways have been discovered within the T. tyrosinosolvens PS2 genome, unlike the above-mentioned representatives of the genus Tsukamurella [ 4 ]. One of these pathways contains an alkane 1-monooxygenase gene ( alkB ; GenBank accession number KZL97795), two rubredoxin genes ( rubA ; GenBank accession numbers KZL97794 and KZL97793), one rubredoxin-reductase gene ( rubB ), and one regulatory protein TetR gene ( alkU ; GenBank accession number KZL97792)[ 4 ].…”

“…Tsukamurella species, which are members of the order Actinomycetales , can be found in a variety of different environments, including soil, water, sludge, and wastewater from petroleum reservoirs; these nonmotile bacilli are obligate aerobic, gram-positive, and weak acid-fast[ 1 - 4 ]. They were initially isolated from bedbug mycetoma and ovaries, in 1941[ 5 ].…”

“…The genus Tsukamurella currently contains 12 species with validly published names that include Tsukamurella tyrosinosolvens ( T. tyrosinosolvens ), and was first described by Collins et al [ 8 ] in 1988. Following a 2016 reclassification of Tsukamurella species, the species T. tyrosinosolvens and Tsukamurella carboxydivorans have been merged[ 4 ]. The genus is phylogenetically related to other mycolic acid-containing genera of the order Actinomycetales , including Nocardia , Gordonia , Streptomyces , Rhodococcus , Corynebacterium , and Mycobacterium , because of their similar phenotypic properties[ 1 , 7 , 9 , 10 ].…”

Hexadecane-induced stress response of hydrocarbon-oxidizing actinobacterial strain Tsukamurella tyrosinosolvens PS2

“…This study was devoted to the effect of hexadecane on the metabolic processes of the Tsukamurella tyrosinosolvens PS2 strain using omics approaches. Methods and Algorithms: The T. tyrosinosolvens PS2 strain was isolated from petrochemical sludge as a potential degrader of linear alkanes [1]. Targeted quantitative proteomics (LC-MRM/MS) of PS2 strain proteins under different cultivation conditions (sucrose and hexadecane) was performed.…”