Draft Genome Sequence of Bacillus velezensis BZR 336g, a Plant Growth-Promoting Antifungal Biocontrol Agent Isolated from Winter Wheat

Garkovenko, Alexey V.; Vasilyev, Ilya; Ilnitskaya, E. V.; Radchenko, V. V.; Асатурова, А. М.; Kozitsyn, Alexander E.; Томашевич, Н. С.; Milovanov, Alexander V.; Григорьева, Т. В.; Штерншис, М. В.

doi:10.1128/mra.00450-20

Cited by 12 publications

(11 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They have been shown to enhance plant growth through a combination of mechanisms [ 15 , 16 ], by activating ISR in the plant against both root and foliar pathogens, by increasing abiotic stress tolerance [ 17 ], and by showing biocontrol properties [ 18 ]. Elicitation of ISR by Bacillus and its metabolites has been demonstrated on a variety of crops to defend against pathogen attack in both the greenhouse and the field [ 19 , 20 ]. It can also produce numerous antifungal compounds [ 21 ], such as lipopeptides [ 22 ], bacillomycin [ 23 ], fengycin [ 24 ], surfactin [ 25 ], bacillibactin [ 26 ], and bacteriocin [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

et al. 2021

View full text Add to dashboard Cite

Plant growth-promoting bacteria (PGPB) are a heterogeneous group of bacteria that can exert beneficial effects on plant growth directly or indirectly by different mechanisms. PGPB-based inoculant formulation has been used to replace chemical fertilizers and pesticides. In our previous studies, two endophytic endospore-forming bacteria identified as Bacillus altitudinis were isolated from roots of Ilex paraguariensis St. Hil. seedlings and selected for their plant growth-promoting (PGP) properties shown in vitro and in vivo. The purposes of this work were to assemble the genomes of B. altitudinis 19RS3 and T5S-T4, using different assemblers available for Windows and Linux and to select the best assembly for each strain. Both genomes were also automatically annotated to detect PGP genes and compare sequences with other genomes reported. Library construction and draft genome sequencing were performed by Macrogen services. Raw reads were filtered using the Trimmomatic tool. Genomes were assembled using SPAdes, ABySS, Velvet, and SOAPdenovo2 assemblers for Linux, and Geneious and CLC Genomics Workbench assemblers for Windows. Assembly evaluation was done by the QUAST tool. The parameters evaluated were the number of contigs ≥ 500 bp and ≥ 1000 bp, the length of the longest contig, and the N50 value. For genome annotation PROKKA, RAST, and KAAS tools were used. The best assembly for both genomes was obtained using Velvet. The B. altitudinis 19RS3 genome was assembled into 15 contigs with an N50 value of 1,943,801 bp. The B. altitudinis T5S-T4 genome was assembled into 24 contigs with an N50 of 344,151 bp. Both genomes comprise several genes related to PGP mechanisms, such as those for nitrogen fixation, iron metabolism, phosphate metabolism, and auxin biosynthesis. The results obtained offer the basis for a better understanding of B. altitudinis 19RS3 and T5S-T4 and make them promissory for bioinoculant development.

show abstract

Section: Introductionmentioning

confidence: 99%

De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In the research we used two initial strains of Bacillus isolated from the rhizosphere of winter wheat in Krasnodar (Kuban Region, Krylovsk district 46 Through genome sequencing of these strains, we identified their taxonomic position. Comparative bioinformation analysis resulted in the collection and deposition of two genomes into NCBI database: B. velezensis BZR 336 g_n (Assembly: GCA_009683125.1, GenBank: NZ_WKKU00000000.1) and B. velezensis BZR 517_n (Assembly: GCA_009683155).1, GenBank: NZ_WKKV00000000.1) [21,22].…”

Section: Bacterial and Fungal Strainsmentioning

confidence: 99%

“…B. velezensis showed great antagonist activity in the south of Russia [18], as well as in the extreme weather of Western Siberia [19,20]. These microorganisms promote plant growth [21], making them excellent candidates for the development of the new bioproducts.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of New Local Bacterial Agents against Pyrenophora tritici-repentis in Kuban Region, Russia

et al. 2022

Self Cite

View full text Add to dashboard Cite

The phytopathogenic fungus Pyrenophora tritici-repentis is a causal agent of tan spot. Antagonistic microorganisms can be used as a non-chemical alternative treatment against the tan spot of wheat. Bacillus velezensis BZR 336 g and BZR 517 stains were selected as the most active microorganisms and potential biocontrol agents. We found that B. velezensis strains BZR 336 g and BZR 517 exhibited antagonistic activity against P. tritici-repentis Kr-15/2016 in vitro: they inhibited mycelium growth by 72.4–94.3% and caused its degenerative changes. Treatment of seeds and plants with strains BZR 336 g and BZR 517 provided a biological efficiency of 31.2–38.4% against tan spot, while artificial inoculation of plants provided only 28.4–43.8% biological efficiency. Treatment of seeds and plants with BZR 336 g and BZR 517 in a three-year field trial demonstrated 24.6–50% biological efficiency. BZR 336 g and BZR 517 provided 5.0–7.6% additional yield. We conclude that BZR 336 g and BZR 517 are promising options for novel bioproducts that can control P. tritici-repentis tan spot.

show abstract

“…The competitiveness and profitability of the livestock industry directly depends on the volume and quality of milk that meets all modern global criteria for its safety. Improving the beneficial properties of milk, its natural value for human nutrition, minimizing losses caused by an insufficiently thought-out technology of keeping, feeding, and milking of animals can be attributed to the most urgent aspects that make it possible to ensure the food security of the Russian Federation considering the ambiguous political and economic situation caused by sanctions from foreign countries and completely cover the population's need for this useful product [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Mass fraction of fat, protein, and their ratio in Simmental breed cows’ milk

Gubanov

Часовщикова

2021

BIO Web Conf.

View full text Add to dashboard Cite

An important role in ensuring the taste and nutritional properties of milk belongs to its chemical composition, particularly the content of fat, protein, vitamins, and minerals. Numerous studies have confirmed the hypothesis of a close relationship between the proportion of milk fat and milk protein, which ideal ration is 1.1: 1.0 - 1.5: 1.0. It is this ratio that not only ensures the harmonious taste of the product but is also an indicator of a complete balanced feeding of animals. The studies were carried out in January - March 2021 at the breeding plant of the Simmental breed in the Tyumen region. The chemical composition of milk was determined in the laboratory for selection control of milk quality of the FSBEI of Higher Education “SAU of the Northern Trans-Urals”. The ratio between the mass fraction of fat and mass fraction of protein on average for the first three months of 2021 can be considered stable. In the milk of 20.0 - 27.8% of cows, there was a shift in equilibrium towards a decrease in butterfat content between the mass fraction of fat and mass fraction of protein, in turn, an increase in butterfat content relative to protein was noted in milk of 2.1 - 3.0% of cows. Equilibrium shift between butterfat and protein over 1.5: 1.0 suggests the risk of ketosis in the herd. In this regard, we recommend carrying out additional diagnostics on the content of ketone bodies in the blood and urine of cows during the milking period to confirm the presumptive diagnosis.

show abstract

Draft Genome Sequence of Bacillus velezensis BZR 336g, a Plant Growth-Promoting Antifungal Biocontrol Agent Isolated from Winter Wheat

Cited by 12 publications

References 20 publications

De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

Efficacy of New Local Bacterial Agents against Pyrenophora tritici-repentis in Kuban Region, Russia

Mass fraction of fat, protein, and their ratio in Simmental breed cows’ milk

Contact Info

Product

Resources

About