Draft Genome Sequence of Bacillus thuringiensis Strain BrMgv02-JM63, a Chitinolytic Bacterium Isolated from Oil-Contaminated Mangrove Soil in Brazil

Marcon, Joelma; Taketani, Rodrigo Gouvêa; Dini‐Andreote, Francisco; Mazzero, Giulia Inocêncio; Soares, Fábio Lino; Melo, I. S. de; Azevedo, João Lúcio de; Andreote, Fernando Dini

doi:10.1128/genomea.01264-13

Cited by 5 publications

(3 citation statements)

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“…Multiple studies have shown the importance of mangroves as source of important enzymes, which may help in the mineralization of organic matter, modulating nutrient cycling in soils [ 41 , 42 , 43 ]. Previously, we have isolated and described the bacterium Bacillus thuringiensis BrMgv02-JM63 from the same oil-contaminated sediment examined in this study and its sequenced genome revealed genes associated with chitinolytic activity [ 15 ]. Furthermore, Dinesh et al [ 44 ] isolated and sequenced the genome of a bacterium affiliated to the genus Mangrovimonas , from an estuarine mangrove, and found genes involved in degradation of xylan.…”

Section: Discussionmentioning

confidence: 99%

“…Enzymes belonging to this family behave as typical exoenzymes, catalysing the cleavage of terminal β-1,4 non-reducing ends of biopolymers [ 13 , 14 ]. These important glycoside hydrolases are known to occur upon two of the most abundant sources of carbon, cellulose and chitin [ 15 ], both present in high amount in the organically enriched soils from mangroves.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Multifunctional β-N-Acetylhexosaminidase Revealed through Metagenomics of an Oil-Spilled Mangrove

et al. 2017

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The use of culture-independent approaches, such as metagenomics, provides complementary access to environmental microbial diversity. Mangrove environments represent a highly complex system with plenty of opportunities for finding singular functions. In this study we performed a functional screening of fosmid libraries obtained from an oil contaminated mangrove site, with the purpose of identifying clones expressing hydrolytic activities. A novel gene coding for a β-N-acetylhexosaminidase with 355 amino acids and 43KDa was retrieved and characterized. The translated sequence showed only 38% similarity to a β-N-acetylhexosaminidase gene in the genome of Veillonella sp. CAG:933, suggesting that it might constitute a novel enzyme. The enzyme was expressed, purified, and characterized for its enzymatic activity on carboxymethyl cellulose, p-Nitrophenyl-2acetamide-2deoxy-β-d-glucopyranoside, p-Nitrophenyl-2acetamide-2deoxy-β-d-galactopyranoside, and 4-Nitrophenyl β-d-glucopyranoside, presenting β-N-acetylglucosaminidase, β-glucosidase, and β-1,4-endoglucanase activities. The enzyme showed optimum activity at 30 °C and pH 5.5. The characterization of the putative novel β-N-acetylglucosaminidase enzyme reflects similarities to characteristics of the environment explored, which differs from milder conditions environments. This work exemplifies the application of cultivation-independent molecular techniques to the mangrove microbiome for obtaining a novel biotechnological product.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Multifunctional β-N-Acetylhexosaminidase Revealed through Metagenomics of an Oil-Spilled Mangrove

et al. 2017

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“…Additionally, genes involved in carbohydrate, energy and amino acid metabolisms are enrich in mangrove sediments [Zhao et al 2019]. In recent decades, bacteria possess different nutrient utilization characteristics, including chitinolytic, lignocellulolytic, cellulose and organic compound degradation, were isolated from worldwide mangrove ecosystems using classical culture methods [Marcon et al 2014;Lam et al 2020;Aziz et al 2018] .…”

Section: Introductionmentioning

confidence: 99%

Thermohalobaculum Xanthum Gen. Nov., Sp. Nov., A Novel Moderately Thermophilic Bacterium Isolated From Mangrove Sediment

Pan

et al. 2021

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A novel moderately thermophilic and halophilic bacterium, designated strain M0105T, was isolated from mangrove sediment collected in the Beibu Gulf, south China. The isolate is Gram-negative, non-motile and rod-shaped bacterium with smooth colonies of pale-yellow appearance. Growth occurs at 15-46℃ (optimum 37-40℃) and pH range of 6.0-10.0 (optimum pH 8.0-9.0). It required 1-7% NaCl (optimum 3-5%) for growth. Strain M0105T was affiliated to the family ‘Rhodobacteraceae’, sharing the highest 16S rRNA gene sequence similarity with Limibaculum halophilum CAU 1123T (96.8%). The major menaquinone Q-10 and the dominant unsaturated fatty acid (C18:1ω7) in this family were also detected in the strain M0105T. The genome sequence possesses a circular 4.1 Mb chromosome with a G+C content of 67.9%. Strain M0105T encoded many genes for cellular stress resistance and nutrient utilization, which could improve its adaptive capacity to the mangrove environment. Values of conserved proteins (POCP), average nucleotide identity (ANI), average amino acid identity (AAI) and DNA-DNA hybridization (dDDH) between the isolate and closely related species were below the proposed threshold for species discrimination. Information from phenotypic, chemotaxonomic and phylogenetic analyses proposed that strain M0105T should be assigned to a novel genus within the family ‘Rhodobacteraceae’. Thus, we suggested that the strain M0105T represents a novel species in a new genus, for which the name Thermohalobaculum xanthum gen. nov., sp. nov. is proposed. The type strain of the type species is M0105T (=BGMRC 2019T=KCTC 52118T =MCCC 1K03767T=NBRC 112057T).Six supplementary figures and two supplementary tables are available with the online version of this article.

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Thermohalobaculum xanthum gen. nov., sp. nov., a moderately thermophilic bacterium isolated from mangrove sediment

Pan

et al. 2021

Antonie van Leeuwenhoek

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Draft Genome Sequence of Bacillus thuringiensis Strain BrMgv02-JM63, a Chitinolytic Bacterium Isolated from Oil-Contaminated Mangrove Soil in Brazil

Cited by 5 publications

References 10 publications

A Novel Multifunctional β-N-Acetylhexosaminidase Revealed through Metagenomics of an Oil-Spilled Mangrove

A Novel Multifunctional β-N-Acetylhexosaminidase Revealed through Metagenomics of an Oil-Spilled Mangrove

Thermohalobaculum Xanthum Gen. Nov., Sp. Nov., A Novel Moderately Thermophilic Bacterium Isolated From Mangrove Sediment

Thermohalobaculum xanthum gen. nov., sp. nov., a moderately thermophilic bacterium isolated from mangrove sediment

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