Draft Genome Sequence of Citrobacter freundii UFMG-H9, Isolated from Urine from a Healthy Bovine Heifer (Gyr Breed)

Giannattasio-Ferraz, Silvia; Maskeri, Laura; Oliveira, André Alves de; Barbosa-Stancioli, Edel Figueiredo; Putonti, Catherine

doi:10.1128/mra.00387-20

Cited by 3 publications

(4 citation statements)

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“…The human UT microbiota is the best studied UT microbiota amongst mammals. Isolation of strains from the bovine UT, including our prior work investigating other bacterial taxa ( Giannattasio-Ferraz et al, 2020a , b , c , d , e , 2021a ) and our study presented here, is a further step toward understanding the healthy bovine UT microbiota. Furthermore, here we have compared these bovine isolates to human UT isolates, producing the first examination of urinary bacteria and urinary phages from two different mammals.…”

Section: Discussionmentioning

confidence: 99%

“…Based upon the 16S rRNA gene sequence, four isolates from four different animals were identified as P. aeruginosa and five isolates, from the same four animals, were identified as E. coli . Isolates of other taxa have been described elsewhere ( Giannattasio-Ferraz et al, 2020a , b , c , d , e , 2021a , b ).…”

Section: Methodsmentioning

confidence: 99%

“…More recently, microbiome studies have found bacteria within the UT of other healthy mammals, including canines ( Burton et al, 2017 ) and swine ( Torres Luque et al, 2020 ). Furthermore, through our prior efforts, we have isolated several different bacterial taxa from the UT of healthy cattle ( Giannattasio-Ferraz et al, 2020a , b , c , d , e , 2021a ).…”

Section: Introductionmentioning

confidence: 99%

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Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

et al. 2022

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The study of livestock microbiota has immediate benefits for animal health as well as mitigating food contamination and emerging pathogens. While prior research has indicated the gastrointestinal tract of cattle as the source for many zoonoses, including Shiga-toxin producing Escherichia coli and antibiotic resistant bacteria, the bovine urinary tract microbiota has yet to be thoroughly investigated. Here, we describe 5 E. coli and 4 Pseudomonas aeruginosa strains isolated from urine of dairy Gyr cattle. While both species are typically associated with urinary tract infections and mastitis, all of the animals sampled were healthy. The bovine urinary strains were compared to E. coli and P. aeruginosa isolates from other bovine samples as well as human urinary samples. While the bovine urinary E. coli isolates had genomic similarity to isolates from the gastrointestinal tract of cattle and other agricultural animals, the bovine urinary P. aeruginosa strains were most similar to human isolates suggesting niche adaptation rather than host adaptation. Examination of prophages harbored by these bovine isolates revealed similarity with prophages within distantly related E. coli and P. aeruginosa isolates from the human urinary tract. This suggests that related urinary phages may persist and/or be shared between mammals. Future studies of the bovine urinary microbiota are needed to ascertain if E. coli and P. aeruginosa are resident members of this niche and/or possible sources for emerging pathogens in humans.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

et al. 2022

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“…The fact that C. phoceense has been primarily detected within the urinary tract of humans and now cattle suggests that this species may be specific to this environment. Recent studies have found that some of the same bacterial species inhabit both the bovine and human urinary microbiota [48,49]. Previous research found that the proteoglycan surface within the human and bovine bladder are similar [50]; in humans, this layer is a source for adhesion and nutrients by urinary bacteria [51].…”

Section: Discussionmentioning

confidence: 99%

Corynebacterium phoceense, resident member of the urogenital microbiota?

et al. 2021

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Corynebacterium phoceense is a Gram-positive species previously isolated from human urine. Although other species from the same genus have been associated with urinary tract infections, C. phoceense is currently believed to be a non-pathogenic member of the urogenital microbiota. Prior to our study, only two isolates were described in the literature, and very little is known about the species. Here, we describe C. phoceense UFMG-H7, the first strain of this species isolated from the urine of healthy cattle. The genome for this isolate was produced and compared to the two other publicly available C. phoceense as well as other Corynebacterium genome assemblies. Our in-depth genomic analysis identified four additional publicly available genome assemblies that are representatives of the species, also isolated from the human urogenital tract. Although none of the strains have been associated with symptoms or disease, numerous genes associated with virulence factors are encoded. In contrast to related Corynebacterium species and Corynebacterium species from the bovine vaginal tract, all C. phoceense strains examined code for the SpaD-type pili suggesting adherence is essential for its persistence within the urinary tract. As the other C. phoceense strains analysed were isolated from the human urogenital tract, our results suggest that this species may be specific to this niche.

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Multidrug-Resistant Klebsiella variicola Isolated in the Urine of Healthy Bovine Heifers, a Potential Risk as an Emerging Human Pathogen

Giannattasio-Ferraz

Ene

Johnson

et al. 2022

Appl Environ Microbiol

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Draft Genome Sequence of Citrobacter freundii UFMG-H9, Isolated from Urine from a Healthy Bovine Heifer (Gyr Breed)

Abstract: Citrobacter freundii is a pathogen associated with antibiotic resistance and severe infections in humans. Here, we report the draft genome sequence of C. freundii strain UFMG-H9, an isolate from urine from a healthy Gyr heifer.

Cited by 3 publications

References 7 publications

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

Corynebacterium phoceense, resident member of the urogenital microbiota?

Multidrug-Resistant Klebsiella variicola Isolated in the Urine of Healthy Bovine Heifers, a Potential Risk as an Emerging Human Pathogen

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