Draft Genome Sequence of Clostridium cochlearium Strain AGROS13, Isolated from a Sheep Dairy Farm in New Zealand

Gupta, Tanushree B.; Maclean, Paul; Jáuregui, Ruy; Risson, Alexis N.; Brightwell, Gale

doi:10.1128/mra.00593-20

Cited by 1 publication

(2 citation statements)

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“…C. galactitolivorans L6 produced the largest VFA profiles as formic, acetic, butyric and isovaleric acids were detected in the spent broth containing skim milk as the main carbon source. C. cochlearium L3, a potential proteolytic organism involved in cheese spoilage (Gupta et al, 2020), produced butyric and acetic acid from NB. This is in accordance with other papers describing this species as producer of both VFA from various protein-rich carbon sources (Nzeteu et al, 2018), which are abundantly present in the NB formulation.…”

Section: Resultsmentioning

confidence: 99%

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Bioaugmentation strategies based on bacterial and methanogenic cultures to relieve stress in anaerobic digestion of protein-rich substrates

Agostini,

Bucci,

Doni

et al. 2023

Preprint

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Anaerobic co-digestion of protein-rich substrates is a prominent strategy for converting valuable feedstocks into methane, but it releases ammonia, which can inhibit methanogenesis. This study developed a cutting-edge combined culturomic and metagenomic approach to investigate the microbial composition of an ammonia-tolerant biogas plant. Newly-isolated microorganisms were used for bioaugmentation of stressed batch reactors fed with casein, maize silage and their combination. A co-culture enriched with proteolytic bacteria was isolated, selected and compared with the proteolytic collection strainPseudomonas lundensisDSM6252. The co-culture andP. lundensiswere combined with the ammonia-resistant archaeonMethanoculleus bourgensisMS2 to boost process stability. A microbial population pre-adapted to casein was also tested for evaluating the digestion of protein-rich feedstock. The promising results suggest combining proteolytic bacteria andM. bourgensiscould exploit microbial co-cultures to improve anaerobic digestion stability and ensure stable productivity even under the harshest of ammonia conditions.Graphical abstractHighlights-High ammonia release from protein-rich substrates inhibits anaerobic digestion-Newly isolated bacterial strains from anaerobic digester were obtained-Proper bioaugmentation alleviates stress in casein and maize silage co-digestion-Bioaugmentation with a hydrolytic/hydrogenotrophic co-culture enhances CH4yields

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Section: Resultsmentioning

confidence: 99%

“…C. cochlearium L3, a potential proteolytic organism involved in cheese spoilage (Gupta et al, 2020), produced butyric and acetic acid from NB. This is in accordance with other papers describing this species as producer of both VFA from various protein-rich carbon sources (Nzeteu et al, 2018), which are abundantly present in the NB formulation.…”

Section: Resultsmentioning

confidence: 99%