Draft Genome Sequence of Enterococcus faecalis Strain CECT7121, a Corn Silage Isolate with Antibacterial Activity against Gram-Positive Pathogens

Delpech, Gastón; Hébert, Elvira María; Sparo, Mónica; Saavedra, Lucila

doi:10.1128/mra.00245-19

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…Enterococcus faecalis CECT7121 (depositada en la Colección Española de Cultivos Tipo, CECT, Burjasot, Valencia, España) es una cepa probiótica productora de un péptido antimicrobiano de 4726 Da con actividad bactericida sobre bacterias Gram positivas denominado AP7121 (Sparo et al, 2006;2009;Delpech et al, 2017;Delpech et al, 2019).…”

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Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121

Lissarrague,

Schofs,

Bistoletti

et al. 2023

BIOTECNIA

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Las infecciones del torrente sanguíneo asociadas a los cuidados de la salud constituyen una de las principales causas de morbilidad y mortalidad. La infección relacionada con catéteres vasculares es su fuente principal. Las bacterias Gram positivas, especialmente Staphylococcus spp., constituyen uno de los agentes etiológicos más frecuentes. Objetivo: evaluar la actividad inhibitoria del AP7121 sobre la adherencia de Staphylococcus aureus en catéteres vasculares. Se utilizó la cepa productora de biofilm S. aureus ATCC 35556 (SA). Se estimó CIMAP7121 para SA, se inocularon segmentos de 20 mm de catéter vascular (n=3) con 104 UFC mL-1 de SA. Se probaron tres esquemas diferentes (A: simultáneo, B: anterior y C: después del desafío bacteriano) utilizando 1 x CIMAP7121. Se incluyeron grupos de control en cada esquema. La CIMAP7121 fue 0,48 mg/L. Con los esquemas A y B se logró una reducción de 2 logaritmos que representa una disminución del 99 % de células viables. El tratamiento posterior a la exposición con AP7121 (esquema C) produjo una reducción de 1 logaritmo que representa una disminución del 90 % de células viables. Estos resultados sugieren una rápida actividad antimicrobiana de AP7121 que podría ser beneficiosa para reducir la adherencia bacteriana en catéteres vasculares.

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Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121

Lissarrague,

Schofs,

Bistoletti

et al. 2023

BIOTECNIA

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Fermentation Quality, Bacterial Community, and Aerobic Stability of Perennial Recut Broussonetia papyrifera Silage with Different Additives and Wilting Time

Jin

Sun

et al. 2022

Fermentation

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Broussonetia papyrifera L. (paper mulberry) is an alternative woody plant, which can used to replace part of the protein feed for ruminants. Ensiling is an effective way to preserve fresh pasture and to solve the problem of stable storage and feed conversion of paper mulberry in the rapid growth period. However, low dry matter (DM), water-soluble carbohydrate, and lactic acid bacteria (LAB) reduce the quality of paper mulberry silage. This study assesses the influence of wilting time (0 h and 3.5 h; lighting: 3.43 × 104 Lux) and three additives (Enterococcus durans, CL; cellulase, CE; and formic acid, FA) on the fermentation quality, aerobic stability, and bacterial community of whole plant B. papyrifera silage. The whole plant B. papyrifera sample was mowed and wilted for 0 h and 3.5 h, and then had CL, CE, or FA added, followed by 60 days of ensiling. The results show all silage samples had high fermentation quality with pH below 4.2, ammonia-nitrogen below 100 g/kg DM, and no detectable butyric acid. The additives protected the DM and the crude protein from protease activity (p < 0.05), and CL was the most effective among them. Furthermore, wilting time influenced the silage’s bacterial communities, but overall, CL treatment had the greatest impact on bacterial communities. Wilting time and formic acid treatment significantly improved aerobic stability (p < 0.05). Enterococcus was positively correlated with lactic acid (LA), while negatively correlated with LA and Weissella (p < 0.001). Enterococcus was identified as the main driver of the whole plant paper mulberry ensiling process in the present study. In conclusion, compared to other additives, LAB is the most effective and economical to improve the fermentation quality and reduce the protein degradation of whole plant paper mulberry silage. Our findings provide a theoretical basis to improve the quality and production of paper mulberry silage.

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Draft Genome Sequence of Enterococcus faecalis Strain CECT7121, a Corn Silage Isolate with Antibacterial Activity against Gram-Positive Pathogens

Abstract: Enterococcus faecalis CECT7121 is a corn silage probiotic bacterium that shows antibacterial activity against Gram-positive pathogens from different origins. Its genome sequence is 2.9 Mb long with a G+C content of 37.3%.

Cited by 2 publications

References 7 publications

Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121

Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121

Fermentation Quality, Bacterial Community, and Aerobic Stability of Perennial Recut Broussonetia papyrifera Silage with Different Additives and Wilting Time

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