Draft Genome Sequence of Erwinia psidii, Causal Agent of Bacterial Blight of Guava (
            <i>Psidium guava</i>
            ) and Dieback of Eucalypt (
            <i>Eucalyptus</i>
            spp.)

Hermenegildo, Pollyane da Silva; Santos, Samuel A.; Guimarães, Lúcio Mauro da Silva; Pereira, Isadora Cristófoli; Vidigal, Pedro Marcus Pereira; Badel, Jorge L.; Alfenas‐Zerbini, Poliane; Máfia, Reginaldo Gonçalves; Ferreira, Maria Lurdes; Alfenas, Acelino C.

doi:10.1128/mra.01598-18

Cited by 5 publications

(11 citation statements)

References 16 publications

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“…citri to form biofilms on abiotic surfaces, but not on plant tissue, is compromised by mutations in the type III secretion genes hrpB, hrpD, and hrpF (Zimaro et al, 2014). These observations suggest that E. psidii may require special conditions, probably mimicking the plant environ- of gene groups are predicted to be missing in the E. psidii strain IBSBF 435 draft genome (Hermenegildo et al, 2019), the absence of the investigated genes must be confirmed. This suggestion is supported by the observation that only a few of the genes that belong to well-characterized clusters (such as ams and fli) were found in the draft sequence.…”

Section: Discussionmentioning

confidence: 97%

“…This suggests that E. psidii may use different mechanisms from those of other plant pathogenic bacteria to form biofilms. Nonetheless, considering that about 2.5% of gene groups are predicted to be missing in the E. psidii strain IBSBF 435 draft genome (Hermenegildo et al , ), the absence of the investigated genes must be confirmed. This suggestion is supported by the observation that only a few of the genes that belong to well‐characterized clusters (such as ams and fli ) were found in the draft sequence.…”

Section: Discussionmentioning

confidence: 99%

“…The nucleotide sequences of the genes reported in those studies were retrieved from the NCBI database (https://www.ncbi.nlm.nih.gov/). Then, BLAST (Altschul et al , ) comparisons were run against the draft genome sequence of E. psidii strain IBSBF 435 (Hermenegildo et al , ) using default parameters.…”

Section: Methodsmentioning

confidence: 99%

“…At least two independent experiments were conducted, using five replicates each. Then, BLAST (Altschul et al, 1990) comparisons were run against the draft genome sequence of E. psidii strain IBSBF 435 (Hermenegildo et al, 2019) using default parameters.…”

Section: Biofilm Formation On Abiotic Surfacesmentioning

confidence: 99%

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Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

et al. 2020

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Dieback and wilt caused by Erwinia psidii is an emerging disease that has been causing considerable damage in eucalypt plantations. Because it is a recently emerged disease, several aspects of the bacterial interaction with its host still remain to be elucidated. In this work, we studied the E. psidii colonization and biofilm formation in eucalypt tissues by specific detection using PCR and scanning electron microscopy (SEM). The results indicate that the bacterium is able to translocate in stem tissue mainly acropetally, although movement in the basipetal direction was also observed to a lesser extent, always through the xylem. No colonization of phloem tissues was observed. In addition to colonizing the xylem, E. psidii colonized the parenchymatous tissue. The bacterium formed cell aggregates enveloped by fibrillar material that evolved into complex, well‐structured biofilms in stem and leaf tissues. In contrast, no biofilm formation was observed on abiotic surfaces. These observations suggest that biofilm formation plays an important role in the elicitation of dieback and wilt symptoms caused by E. psidii on eucalypt plants. This study not only shows ultrastructural aspects of the E. psidii communities but also tissue damage in eucalypt plants that was associated with the presence of bacterial aggregates and formation of tyloses.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Biofilm Formation On Abiotic Surfacesmentioning

confidence: 99%

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Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

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“…The draft genome sequence of E . psidii type strain IBSBF435 has been deposited in the NCBI database under the accession number RHHM00000000 (Hermenegildo et al, 2019). The sequences of the other three strains will be made publicly available upon publication elsewhere.…”

Section: Methodsmentioning

confidence: 99%

Prediction, structure characterization, and evolutionary analysis of Erwinia psidii putative type III effectors

et al. 2020

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Erwinia psidii is a gram‐negative bacterium that threatens both guava and eucalypt plantations in several countries. Despite the economic importance of both crops, nothing is currently known about the molecular mechanisms underlying E. psidii pathogenicity and, consequently, how it evolved to infect Eucalyptus species besides its presumed native host Psidium guajava. In this study, we predicted putative type III secretion system effectors that may play important roles during plant–E. psidii interactions and conducted effector structure and phylogenetic analyses to gain important insights into their function and evolution. For that, the whole genomes of four E. psidii strains that exhibit differential aggressiveness towards eucalypt clones were sequenced and their effector repertoires predicted based on sequence identity with known effectors of the model phytopathogen Erwinia amylovora. Only proteins sharing significant sequence identity with the DspE and Eop1 effectors were found. Here, it is shown that these two E. psidii effectors retain all structural characteristics of their corresponding protein superfamilies, but exhibit allelic variations that are consistent with the observed aggressiveness differences between strains. Phylogenetic analyses revealed that whereas E. psidii housekeeping gene sequences are more closely related to those from Erwinia tracheiphila, the effector (either nucleotide or amino acid) sequences are more closely related to their Pantoea agglomerans counterparts, suggesting that dspE and eop1 were both acquired through horizontal gene transfer from the latter bacterial species. The results of this study provide important insights on E. psidii pathogenicity and set the stage for future effector functional studies.

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Sensitive detection, quantification, and monitoring of Erwinia psidii colonization of guava plants using intercalating dye-based real-time PCR

Hermenegildo

Freitas

CascardoCascardo

et al. 2022

Trop. plant pathol.

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Draft Genome Sequence of Erwinia psidii, Causal Agent of Bacterial Blight of Guava ( Psidium guava ) and Dieback of Eucalypt ( Eucalyptus spp.)

Abstract: Here, we present a draft genome sequence of the type strain IBSBF 435 of Erwinia psidii (Enterobacteriaceae), a phytopathogen that causes bacterial blight on guava (Psidium guava) and dieback and wilt on eucalypt (Eucalyptus spp.), both of which are important emerging diseases.

Cited by 5 publications

References 16 publications

Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

Prediction, structure characterization, and evolutionary analysis of Erwinia psidii putative type III effectors

Sensitive detection, quantification, and monitoring of Erwinia psidii colonization of guava plants using intercalating dye-based real-time PCR

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