Draft Genome Sequence of Escherichia coli K-12 (ATCC 29425)

Engelbrecht, Kathleen; Putonti, Catherine; Koenig, David W.; Wolfe, Alan J.

doi:10.1128/genomea.00574-17

Cited by 5 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study demonstrated high disinfection performance against E. coli ATCC 29425 (99.99% reduction). This can be attributed to relatively low %GC content of E. coli (50.68%; Engelbrecht et al, 2017) and its peak UVC sensitivity (Green et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Disinfection Performance of a Drinking Water Bottle System With a UV Subtype C LED Cap Against Waterborne Pathogens and Heterotrophic Contaminants

Mariita

Blumenstein

Beckert³

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

The purgaty One systems (cap+bottle) are portable stainless-steel water bottles with UV subtype C (UVC) disinfection capability. This study examines the bottle design, verifies disinfection performance against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae, and heterotrophic contaminants, and addresses the public health relevance of heterotrophic bacteria. Bottles were inoculated with deliberately contaminated potable water and disinfection efficacy examined using colony forming unit (CFU) assay for each bacterial strain. The heterotrophic plate count (HPC) method was used to determine the disinfection performance against environmental contaminants at day 0 and after 3days of water in stationary condition without prior UVC exposure. All UVC irradiation experiments were performed under stationary conditions to confirm that the preset application cycle of 55s offers the desired disinfection performance under-tested conditions. To determine effectiveness of purgaty One systems (cap+bottle) in disinfection, inactivation efficacy or log reduction value (LRV) was determined using bacteria concentration between UVC ON condition and controls (UVC OFF). The study utilized the 16S ribosomal RNA (rRNA) gene for characterization of isolates by identifying HPC bacteria to confirm if they belong to groups that are of public health concern. Purgaty One systems fitted with Klaran UVC LEDs achieved 99.99% inactivation (LRV4) efficacy against E. coli and 99.9% inactivation (LRV3) against P. aeruginosa, V. cholerae, and heterotrophic contaminants. Based on the 16S rRNA gene analyses, the study determined that the identified HPC isolates from UVC irradiated water are of rare public health concern. The bottles satisfactorily inactivated the target pathogenic bacteria and HPC contaminants even after 3days of water in stationary condition.

show abstract

Section: Discussionmentioning

confidence: 99%

Disinfection Performance of a Drinking Water Bottle System With a UV Subtype C LED Cap Against Waterborne Pathogens and Heterotrophic Contaminants

Mariita

Blumenstein

Beckert³

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The study demonstrated high disinfection performance against E. coli ATCC 29425 (99.99% reduction). This can be attributed to relatively low %GC content of E. coli (50.68%) [23] and its peak UVC sensitivity [24]. Although the Vibrio Genus has low %GC content (~47%), the presence of more elaborate DNA repair/protection processes make them less susceptible against UVC irradiation [25], thus obtaining 99.9% disinfection under similar test conditions (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Disinfection performance of a drinking water bottle system with a UVC LED cap against waterborne pathogens and heterotrophic contaminants

Mariita

Blumenstein

Beckert³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The purgaty One systems (cap+bottle) are portable stainless-steel water bottles with ultraviolet subtype C (UVC) disinfection capability. This study examines the bottle design, verifies disinfection performance against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and heterotrophic contaminants and addresses the public health relevance of heterotrophic bacteria. Methods: Bottles were inoculated with bacterial strains and disinfection efficacy examined using colony forming unit (CFU) assay. The heterotrophic plate count (HPC) method was used to determine the disinfection performance against environmental contaminants at day 0 and after 3 days of water stagnation. All UVC irradiation experiments were performed under stagnant conditions to confirm that the preset application cycle of 55 seconds offers the desired disinfection performance under worst-case condition. To determine the effectiveness of purgaty One systems (cap+bottle) in disinfection, inactivation efficacy or log reduction value (LRV) was determined using bacteria concentration between UVC ON condition and controls (UVC OFF). The study utilized the 16S rRNA gene for isolate characterization by identifying HPC bacteria to confirm if they belong to groups that are of public health concern. Results: Purgaty One systems fitted with Klaran UVC LEDs achieved 99.99% inactivation (LRV4) efficacy against E. coli and 99.9% inactivation (LRV3) against P. aeruginosa, V. cholerae and heterotrophic contaminants. Based on the 16S rRNA gene analyses, the study determined that the identified HPC isolates enriched by UVC irradiation are of rare public health concern. Conclusion: The bottles satisfactorily inactivated the target pathogenic bacteria and HPC contaminants even after 3 days of water stagnation.

show abstract

“…Standard curves of the target genes KmR / dxs for strain pJ281 and nptII / dxs for strain BW25113 were respectively created using a ten-fold serial dilution series spanning a range of 1 × 10 3 to 1 × 10 8 copies/µL of plasmid DNA and genomic DNA. Genomic DNA sizes were calculated to be 4,534,037 bp for E. coli DH5α (pJ281) and 4,639,221 bp for E. coli K12 (BW25113), as reported by Song et al [ 22 ] and Engelbrecht et al [ 23 ], respectively. Following the description of Whelan et al [ 24 ], the DNA copy numbers/µL was calculated using the following formula: DNA copy numbers/µL = (6.02 × 10 23 [copies/mol] × DNA amount [g])/(DNA length [bp] × 660 [g/mol/bp]), …”

Section: Methodsmentioning

confidence: 99%

Rapid Monitoring of Viable Genetically Modified Escherichia coli Using a Cell-Direct Quantitative PCR Method Combined with Propidium Monoazide Treatment

Qin

Lee

2023

Microorganisms

View full text Add to dashboard Cite

The commercialization of industrial genetically modified microorganisms (GMMs) has highlighted their impact on public health and the environment. Rapid and effective monitoring methods detecting live GMMs are essential to enhance current safety management protocols. This study aims to develop a novel cell-direct quantitative polymerase chain reaction (qPCR) method targeting two antibiotic-resistant genes, KmR and nptII, conferring resistance against kanamycin and neomycin, along with propidium monoazide, to precisely detect viable Escherichia coli. The E. coli single-copy taxon-specific gene of D-1-deoxyxylulose 5-phosphate synthase (dxs) was used as the internal control. The qPCR assays demonstrated good performance, with dual-plex primer/probe combinations exhibiting specificity, absence of matrix effects, linear dynamic ranges with acceptable amplification efficiencies, and repeatability for DNA, cells, and PMA-treated cells targeting KmR/dxs and nptII/dxs. Following the PMA-qPCR assays, the viable cell counts for KmR-resistant and nptII-resistant E. coli strains exhibited a bias% of 24.09% and 0.49%, respectively, which were within the acceptable limit of ±25%, as specified by the European Network of GMO Laboratories. This method successfully established detection limits of 69 and 67 viable genetically modified E. coli cells targeting KmR and nptII, respectively. This provides a feasible monitoring approach as an alternative to DNA processing techniques to detect viable GMMs.

show abstract

Draft Genome Sequence of Escherichia coli K-12 (ATCC 29425)

Cited by 5 publications

References 4 publications

Disinfection Performance of a Drinking Water Bottle System With a UV Subtype C LED Cap Against Waterborne Pathogens and Heterotrophic Contaminants

Disinfection Performance of a Drinking Water Bottle System With a UV Subtype C LED Cap Against Waterborne Pathogens and Heterotrophic Contaminants

Disinfection performance of a drinking water bottle system with a UVC LED cap against waterborne pathogens and heterotrophic contaminants

Rapid Monitoring of Viable Genetically Modified Escherichia coli Using a Cell-Direct Quantitative PCR Method Combined with Propidium Monoazide Treatment

Contact Info

Product

Resources

About