Draft genome sequence of Escherichia coli M51-3: a multidrug-resistant strain assigned as ST131-H30 recovered from infant diarrheal infection in Mexico

Magaña-Lizárraga, José Antonio; Ahumada-Santos, Yesmi Patricia; Parra‐Unda, Jesús Ricardo; Uribe-Beltrán, Magdalena de Jesús; Vega-López, Inés F.; Prieto-Alvarado, Rogelio; Báez-Flores, María Elena

doi:10.1016/j.jgar.2019.10.030

Cited by 2 publications

(2 citation statements)

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“…In addition, Yersiniabactin virulence factors commonly associated with urinary tract infections were also identified in E. coli isolates[40]. In addition, the presence of senB enterotoxin implicated in the development of severe diarrhea among patient infected with enteroinvasive E. coli and shigella was also reported [41]. In the S. aureus isolate, capsular polysaccharide, cap8A-P identified.…”

Section: Discussionmentioning

confidence: 99%

“…These capsular polysaccharides are covalently attached to peptidoglycan and play several roles including biofilm formation, colonization and evading phagocytes uptakes and protecting important bacterial cell wall constituents [42]. Other virulence factors such as enterotoxins hlgA-C is a cytolytic pore forming toxin that kills polymorphonuclear phagocytes, disrupt endothelial and epithelial barriers [41].…”

Section: Discussionmentioning

confidence: 99%

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Application of nanopore sequencing to identify antimicrobial resistance genes, mobile genetic elements and virulence factors in clinical isolates

Kimani,

Musundi,

Wakaba

et al. 2023

Preprint

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The global health challenge posed by the emergence of antibiotic resistance pathogen is further exacerbated in African countries by the indiscriminate use of antibiotics, poor surveillance and lack of stewardship programs. To address this issue, we employed the Oxford Nanopore Technologies (ONT) to sequence 17 clinical isolates from a referral hospital in Kenya. Our comprehensive bioinformatics approach facilitated the assembly, identification of sequence types and prediction of antimicrobial resistance genes, mobile genetic elements (plasmids and integrons) and virulence genes. Of the 17 isolates, five were A. baumannii, four E. coli, three S. haemolyticus, three were E. cloacae, while S. aureus and E. faecalis were single isolates. For the detection of AMR genes, A. baumannii isolates harbored genes such as blaOXA-23 which mediates resistance to carbapenems, E. coli and E. cloacae carried blaCTX-M-15 which confers resistance to cephalosporins and S. haemolyticus harbored blaZ, responsible for resistance against penicillins. S. aureus co-haboured mecA and blaZ genes. In addition,, various other different AMR genes to chloramphenicol, macrolides, aminoglycosides, tetracycline were also observed. For plasmid replicons, E. coli carried the most number of plasmids and shared ColRNAI_1 and IncFIB(pB171)_1_pB171 with A. baumannii and IncR_1 with E. cloacae. Many genes encoding various virulence factors including fimA-I and ompA,senB were identified in E. coli,hlgA-C and hla/hly, hlb, hld in S. aureus and efaA, ebpA-C in E. faecalis. In conclusion, most isolates contained a combination of different AMR genes harbored in plasmids and integrons and virulence genes. This study provides significant information on genetic determinants of antibiotic resistant pathogens in clinical isolates and could assist in developing strategies that improve patient treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of nanopore sequencing to identify antimicrobial resistance genes, mobile genetic elements and virulence factors in clinical isolates

Kimani,

Musundi,

Wakaba

et al. 2023

Preprint

View full text Add to dashboard Cite

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Comparative Genomics of Three Hybrid-Pathogen Multidrug-Resistant Escherichia coli Strains Isolated from Healthy Donors’ Feces

Ortega-Enríquez,

Martínez-de la Peña,

Lara-Ochoa

et al. 2024

Microbiology Research

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The present study shows the genomic characterization of three pathogenic Escherichia coli hybrid strains. All strains were previously characterized as diarrheagenic pathotypes (DEC), obtained from feces. The three sequenced strains have genes that encode adhesins (fimH and iha) and iron uptake systems (iucC and iutA). Antibiotic resistance genes were also found for fluoroquinolone and aminoglycoside families in the three strains. The presence of genomic islands (GIs) in the sequenced study strains presented 100% identity (Ec-25.2) and 99% identity (Ec-36.1) with previously reported Extraintestinal Pathogenic E. coli (ExPEC) strains. The Ec-36.4 strain shared a 99% identity with GI from the Enterotoxigenic E. coli (ETEC) pathotype of the diarrheagenic E. coli strain. Ec-25.2 belongs to ST69 and harbors a FimH27 variant, while Ec-36.1 and Ec-36.4 belong to ST4238 and share a FimH54 variant. Four incompatibility groups associated with conjugative plasmids were identified (IncFIB, IncF11, IncI1, and IncB/O/K/Z), as well as Insertion Sequences and MITEs elements.

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Draft genome sequence of Escherichia coli M51-3: a multidrug-resistant strain assigned as ST131-H30 recovered from infant diarrheal infection in Mexico

Cited by 2 publications

References 5 publications

Application of nanopore sequencing to identify antimicrobial resistance genes, mobile genetic elements and virulence factors in clinical isolates

Application of nanopore sequencing to identify antimicrobial resistance genes, mobile genetic elements and virulence factors in clinical isolates

Comparative Genomics of Three Hybrid-Pathogen Multidrug-Resistant Escherichia coli Strains Isolated from Healthy Donors’ Feces

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