Draft Genome Sequence of Halomonas hydrothermalis MTCC 5445, Isolated from the West Coast of India

Sv, Vamsi Bharadwaj; Shrivastav, Anupama; Dubey, Sonam; Ghosh, Tonmoy; Paliwal, Chetan; Maurya, Rahulkumar; Mishra, Sandhya

doi:10.1128/genomea.01419-14

Cited by 8 publications

(7 citation statements)

References 7 publications

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“…Halomonas hydrothermalis MTCC 5445 with NCBI Genbank accession no. GU938192 isolated from Aadri Veraval, Gujarat (20°57.584′ N, 70°16.659′ E) 33 has been used in current study. It was maintained in Zobell marine agar (ZMA) slants at 4 °C until use.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

1-Ethyl-3-methylimidazolium Diethylphosphate Based Extraction of Bioplastic “Polyhydroxyalkanoates” from Bacteria: Green and Sustainable Approach

Dubey

Bharmoria

Gehlot

et al. 2017

ACS Sustainable Chem. Eng.

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An eco-friendly approach toward downstream processing of bacterial biomass for extraction of an intracellular potential bioplastic material polyhydroxyalkanoates replacing chlorinated organic solvents is reported using ionic liquid (IL)dissolved wet bacterial biomass of Halomonas hydrothermalis (MTCC accession no. 5445; NCBI Genbank accession no. GU938192), with ease on account of its high hydrogen bond basicity (β = 1.07). The recovered polymer with a yield of 60% ± 2% was characterized using 1 H NMR, FT-IR, and TGA techniques and confirmed to be polyhydroxybutyrate (PHB). The properties of PHB were found to be in close proximity to the standard PHB. The [C 2 mim][(C 2 ) 2 OPO 3 ] was recovered with 60% yield and retention of chemical structure after two consecutive dissolution cycles, which minimized the cost of developed process. The recovered PHB was used to prepare a bioplastic film, which showed good thermal and mechanical stability, as characterized by FT-IR, DSC, TGA, and DMA techniques.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

1-Ethyl-3-methylimidazolium Diethylphosphate Based Extraction of Bioplastic “Polyhydroxyalkanoates” from Bacteria: Green and Sustainable Approach

Dubey

Bharmoria

Gehlot

et al. 2017

ACS Sustainable Chem. Eng.

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“…3 ) ( Grigoriev, 1998 ). The comparative analysis of gene locations between A020, Halomonas hydrothermalis ( Bharadwaj Sv et al , 2015 ), and H. meridiana ( Meyer et al , 2015 ) revealed genome translocation in each case at positions symmetric to the axes of ori and ter . Among the 3,673 protein-coding genes, 3,177 were well conserved (e value <10 –5 ) among Halomonas strains, 369 were conserved among certain Halomonas and other bacterial strains, and 127 did not exhibit similarities in databases.…”

Section: Resultsmentioning

confidence: 99%

“…3) (Grigoriev, 1998). The comparative analysis of gene locations between A020, Halomonas hydrothermalis (Bharadwaj Sv et al, 2015), and H. meridiana (Meyer et al, 2015) revealed genome translocation in each case at A) Eleven Halomonas isolates and a control strain (Halomonas sp. KM-1) were cultured on SOT agar media (2.5% NaCl, pH 9.5) supplemented with 3% sucrose at 33°C (basic conditions).…”

Section: Whole-genome Analysismentioning

confidence: 99%

“… The positions of the orthologs, which were defined based on a Basic Local Alignment Search Tool (BLAST) bit score >50 and reciprocal best hits, between the genomes of Halomonas sp. A020, Halomonas hydrothermalis ( Bharadwaj Sv et al , 2015 ), Halomonas sp. A020, and Halomonas meridiana ( Meyer et al , 2015 ) are indicated at the top and bottom.…”

Section: Figmentioning

confidence: 99%

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Identification of New <i>Halomonas</i> Strains from Food-related Environments

et al. 2022

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Halomonas species, which are aerobic, alkaliphilic, and moderately halophilic bacteria, produce diverse biochemicals. To identify food-related Halomonas strains for bioremediation and the industrial production of biochemicals, 20 strains were isolated from edible seashells, shrimp, and umeboshi (pickled Japanese plum) factory effluents. All isolates were phylogenetically classified into a large clade of Halomonas species. Most isolates, which grew in wide pH (6-13) and salt concentration (0-14%) ranges, exhibited the intracellular accumulation of poly(3-hydroxybutyrate) granules. The characteristics of these isolates varied. A020 isolated from umeboshi factory effluents exhibited enhanced stress tolerance and proliferation and comprised two plasmids. IMZ03 and A020 grew to more than 200 OD 600 , while IMZ03 produced 3.5% 3-hydroxybutyrate in inorganic medium supplemented with 10% sucrose. The mucus of TK1-1 cultured on agar medium comprised approximately 64 mM of ectoine. Whole-genome sequencing of A020 was performed to elucidate its origin and genomic characteristics. The genome analysis revealed a region exhibiting synteny with a large virus genome isolated from the ocean, but did not identify any predictable pathogenic genes. Therefore, saline foods and related materials may be suitable resources for isolating Halomonas strains exhibiting unique, useful, and innocuous features.

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“…A similar growth trend was observed at different time intervals with varying concentration which depicts the optimum time of harvesting to be 48 h and 72 h for H. daqingensis and H. ventosae respectively. Whole genome sequenced Halomonas hydrothermalis is reported to accumulate co-polymer when fed with crude levulinic acid and Jatropha biodiesel wastes ( Bera et al, 2015 ; Bharadwaj et al, 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Efficient Production of Polyhydroxyalkanoate Through Halophilic Bacteria Utilizing Algal Biodiesel Waste Residue

Dubey

Mishra

2021

Front. Bioeng. Biotechnol.

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The objective of the current work was to investigate the potential of halophilic bacterial isolates for efficient utilization of crude glycerol from algal biodiesel waste into polyhydroxyalkanoates (PHAs) a green plastic. Screening of the isolates was directly done in algal biodiesel waste residue containing solid agar plates supplemented with Nile red. Crude glycerol is a biodiesel waste whose bioconversion into value-added products provides an alternative for efficient management with dual benefit. For the scale-up studies of PHAs, Halomonas spp. especially H. daqingensis was observed as a potential candidate growing well in 3% Algal biodiesel waste residue (ABWR), 5% NaCl supplementation at 35°C within 48 h of incubation. Maximum Cell dry weight (CDW) of 0.362 ± 0.001 g and 0.236 ± 0.003 g PHA was obtained with H. daqingensis when grown in the fermentor with 0.5 vvm air flow rate and 200 rpm containing 3% ABWR supplemented with 5% NaCl at 35°C incubation temperature for 48 h. ABWR can serve as a sole substrate for PHA production at an industrial scale serving two approaches: getting rid of the biodiesel industrial waste containing high amount of glycerol besides using waste replacing commercial substrate thereby reducing the cost of the product.

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Draft Genome Sequence of Halomonas hydrothermalis MTCC 5445, Isolated from the West Coast of India

Cited by 8 publications

References 7 publications

1-Ethyl-3-methylimidazolium Diethylphosphate Based Extraction of Bioplastic “Polyhydroxyalkanoates” from Bacteria: Green and Sustainable Approach

1-Ethyl-3-methylimidazolium Diethylphosphate Based Extraction of Bioplastic “Polyhydroxyalkanoates” from Bacteria: Green and Sustainable Approach

Identification of New <i>Halomonas</i> Strains from Food-related Environments

Efficient Production of Polyhydroxyalkanoate Through Halophilic Bacteria Utilizing Algal Biodiesel Waste Residue

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