Draft Genome Sequence of Andreprevotia sp. Strain IGB-42, a Chitinolytic Bacterium Isolated from a Soil Sample of an Anthill in Stuttgart, Germany

Abstract

Yevhen Vainshtein and Nicole Werner contributed equally to this work. The order of authors was determined based on a coin toss.ABSTRACT Andreprevotia sp. strain IGB-42 is a chitin-degrading bacterium isolated from the soil of an anthill. The genome contains 4.7 Mb, a GϩC content of 61.31%, 4,257 predicted open reading frames, and a set of industrially interesting chitinase genes.

Genomic and Proteomic Study of Andreprevotia ripae Isolated from an Anthill Reveals an Extensive Repertoire of Chitinolytic Enzymes

“…The genome of A. ripae was assembled from 52 contigs leading to a genome size of 4.7 Mb and a G+C content of 61.3%. 21 Gene annotation using Prokka version 1.14.1 yielded 4257 potential open reading frames, of which 587 (13.8%) were predicted to encode for secreted proteins. A search with dbCAN 29 showed that the genome encodes for 188 putative carbohydrate-active enzymes (4.4%), including 74 glycosyl hydrolases (GHs), 45 glycosyl transferases (GT), 29 carbohydrate esterases (CE), 3 polysaccharide lyases (PL), one lytic polysaccharide monooxygenase (LPMO), 9 proteins with other auxiliary activities (AA), and 27 proteins containing a carbohydrate-binding module (CBM) but with no predicted carbohydrate-active catalytic function.…”

“…A. ripae IGB-42 21 was grown on M9 minimal medium plates containing 1% (w/v) milled α-chitin (Seagarden, Husøyvegen 278, 4262 Avaldsnes, Norway), 1% (w/v) glucose (VWR International, Radnor, Pennsylvania, PA, USA), or 1% (w/v) N -acetylglucosamine (NAG) (VWR International, Radnor, Pennsylvania, PA, USA) as sole carbon source. The plates were incubated at 22 °C, with three biological replicates for each time point and substrate.…”

“…In search of novel and potentially more advanced chitinolytic machineries, we turned to Andreprevotia ripae ( A. ripae ), a Gram-negative bacterium that was isolated from an abandoned anthill. 21 Anthills are rich in chitin, which is present in insect remains and fungi that accumulate inside the hill. We have carried out a detailed analysis of the predicted chitinolytic machinery encoded by the genome of A. ripae , 21 showing the presence of a large number of carbohydrate-active enzymes (CAZymes), including an unprecedented high number of putative chitinases.…”

“… 21 Anthills are rich in chitin, which is present in insect remains and fungi that accumulate inside the hill. We have carried out a detailed analysis of the predicted chitinolytic machinery encoded by the genome of A. ripae , 21 showing the presence of a large number of carbohydrate-active enzymes (CAZymes), including an unprecedented high number of putative chitinases. We then assessed how A. ripae employs this rich arsenal of enzymes, by studying the secretome of A. ripae when grown on chitin, compared to when grown on N -acetylglucosamine or glucose.…”

“…In search of novel and potentially more advanced chitinolytic machineries, we turned to Andreprevotia ripae ( A. ripae ), a Gram-negative bacterium that was isolated from an abandoned anthill . Anthills are rich in chitin, which is present in insect remains and fungi that accumulate inside the hill.…”