Draft Genome Sequence of
            <i>Asaia</i>
            sp. Strain SF2.1, an Important Member of the Microbiome of
            <i>Anopheles</i>
            Mosquitoes

Shane, Jackie; Bongio, Nicholas J.; Favia, Guido; Lampe, David J.

doi:10.1128/genomea.01202-13

Cited by 11 publications

(12 citation statements)

References 13 publications

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“…Signal sequences predicted by PRED-TAT are in bold. Protein identification was made using NCBI BLAST focusing on the Asaia SF2.1 genome sequence [ 38 ]. If a hypothetical protein was detected with the Asaia genome, another BLAST was performed using the entire NCBI database.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Plasmodium berghei Development in Mosquitoes by Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel Native Secretion Signal

Bongio

Lampe

2015

PLoS ONE

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Novel interventions are needed to prevent the transmission of the Plasmodium parasites that cause malaria. One possible method is to supply mosquitoes with antiplasmodial effector proteins from bacteria by paratransgenesis. Mosquitoes have a diverse complement of midgut microbiota including the Gram-negative bacteria Asaia bogorensis. This study presents the first use of Asaia sp. bacteria for paratransgenesis against P. berghei. We identified putative secreted proteins from A. bogorensis by a genetic screen using alkaline phosphatase gene fusions. Two were secreted efficiently: a siderophore receptor protein and a YVTN beta-propeller repeat protein. The siderophore receptor gene was fused with antiplasmodial effector genes including the scorpine antimicrobial peptide and an anti-Pbs21 scFv-Shiva1 immunotoxin. Asaia SF2.1 secreting these fusion proteins were fed to mosquitoes and challenged with Plasmodium berghei-infected blood. With each of these effector constructs, significant inhibition of parasite development was observed. These results provide a novel and promising intervention against malaria transmission.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Plasmodium berghei Development in Mosquitoes by Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel Native Secretion Signal

Bongio

Lampe

2015

PLoS ONE

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“…sp. SF2.1 (NCBI assembly ID GCF_000505765.1), 58 Asaia prunellae JCM 25354 (GCF_000613885.1), As. platycodi JCM 25414 (GCF_000614545.1), and As.…”

Section: Discussionmentioning

confidence: 99%

Complete genome and gene expression analyses ofAsaia bogorensisreveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen

Kawai

Higashiura

Hayasaki

et al. 2015

DNA Res

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Asaia bogorensis, a member of acetic acid bacteria (AAB), is an aerobic bacterium isolated from flowers and fruits, as well as an opportunistic pathogen that causes human peritonitis and bacteraemia. Here, we determined the complete genomic sequence of the As. bogorensis type strain NBRC 16594, and conducted comparative analyses of gene expression under different conditions of co-culture with mammalian cells and standard AAB culture. The genome of As. bogorensis contained 2,758 protein-coding genes within a circular chromosome of 3,198,265 bp. There were two complete operons encoding cytochrome bo3-type ubiquinol terminal oxidases: cyoABCD-1 and cyoABCD-2. The cyoABCD-1 operon was phylogenetically common to AAB genomes, whereas the cyoABCD-2 operon belonged to a lineage distinctive from the cyoABCD-1 operon. Interestingly, cyoABCD-1 was less expressed under co-culture conditions than under the AAB culture conditions, whereas the converse was true for cyoABCD-2. Asaia bogorensis shared pathogenesis-related genes with another pathogenic AAB, Granulibacter bethesdensis, including a gene coding pathogen-specific large bacterial adhesin and additional genes for the inhibition of oxidation and antibiotic resistance. Expression alteration of the respiratory chain and unique hypothetical genes may be key traits that enable the bacterium to survive under the co-culture conditions.

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“…Acetate and lactate are oxidized to carbon dioxide and water but the activity was considered low. Partial sequences of nifH ‐like genes from several isolates and Asaia type strains were obtained and, as well as their genomes , are deposited at GenBank . Noteworthy, up to date blast analysis revealed that these sequences blast only among them and with several partial nifH ‐like sequences from unculturable clone or chlorophyllide reductase subunit X ( bchX ) partial gene sequences from several microbial genome or unculturable clones (Fig.…”

Section: Asaiamentioning

confidence: 99%

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Reis

Teixeira

2015

J. Basic Microbiol.

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For centuries, the Acetobacteraceae is known as a family that harbors many species of organisms of biotechnological importance for industry. Nonetheless, since 1988 representatives of this family have also been described as nitrogen fixing bacteria able to plant growth promotion by a variety of mechanisms. Nitrogen fixation is a biological process that guarantees that the atmospheric N2 is incorporated into organic matter by several bacterial groups. Most representatives of this group, also known as diazotrophic, are generally associated with soil rhizosphere of many plants and also establishing a more specific association living inside roots, leaves, and others plants tissues as endophyte. Their roles as plant growth-promoting microorganisms are generally related to increase in plant biomass, phosphate and other mineral solubilization, and plant pathogen control. Here, we report many of these plant growth-promoting processes related to nitrogen fixing species already described in Acetobacteraceae family, especially Gluconacetobacter diazotrophicus and their importance to agriculture. In addition, a brief review of the state of art of the phylogenetics, main physiological and biochemical characteristics, molecular and functional genomic data of this group of Acetobacteraceae is presented.

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Draft Genome Sequence of Asaia sp. Strain SF2.1, an Important Member of the Microbiome of Anopheles Mosquitoes

Cited by 11 publications

References 13 publications

Inhibition of Plasmodium berghei Development in Mosquitoes by Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel Native Secretion Signal

Inhibition of Plasmodium berghei Development in Mosquitoes by Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel Native Secretion Signal

Complete genome and gene expression analyses ofAsaia bogorensisreveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

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