Draft Genome Sequence of
            <i>Brevibacillus</i>
            sp. Strain BAB-2500, a Strain That Might Play an Important Role in Agriculture

Joshi, Madhvi; Sharma, Anukriti; Pandit, A. S.; Pandya, Rohan V.; Saxena, Anupam; Bagatharia, S. B.

doi:10.1128/genomea.00021-13

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…The present 3D structure and catalytic insights to the ST15c10 endoglucanase support our biochemical experimentation related to enzymatic regulations. The total of 2 sequences on the keyword glucanase/cellulase of any B. agri are available from the NCBI protein/gene data bank (GenBank: ELK43520 [350 aa], and ELK43255 [359 aa]), which were very recently resolved from the B. agri BAB-2500 whole genome sequence [Joshi et al, 2013]. The celM aminopeptidase M42 family was also resolved as endoglucanases and updated very recently in C. novyi NT (gene ID: 4540770) and C. botulinum BKT015925 (gene ID: 10465465).…”

Section: Discussionmentioning

confidence: 99%

The Dual Carboxymethyl Cellulase and Gelatinase Activities of a Newly Isolated Protein from Brevibacillus agri ST15c10 Confer Reciprocal Regulations in Substrate Utilization

Maiti

Samanta²,

Sahoo³

et al. 2017

Microb Physiol

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A protein showing endoglucanase-peptidase activity was prepared from a newly isolated bacterium (ST15c10). We identified ST15c10 as Brevibacillus agri based on electron-microscopic images and its 16S-rDNA sequence (GenBank accession No. HM446043), which exhibits 98.9% sequence identity to B. agri (KZ17)/B. formosus (DSM-9885T)/B. brevis. The enzyme was purified to homogeneity and gave a single peak during high-performance liquid chromatography on a Seralose 6B-150 gel-matrix/C-18 column. MALDI-TOF mass-spectrometry and bioinformatics studies revealed significant similarity to M42-aminopeptidases/endoglucanases of the CelM family. These enzymes are found in all Brevibacillus strains for which the genome sequence is known. ST15c10 grows optimally on carboxymethyl cellulose (CMC)-gelatin (40°C/pH 8-9), and also shows strong growth/carboxymethyl cellulase (CMCase) activity in submerged bagasse fermentation. The purified enzyme also functions as endoglucanase with solid bagasse/rice straw. Its CMCase activity (optimal at pH 5.6 and 60°C/K_m = 35.5 µM/V_max = 1,024U) was visualized by zymography on a CMC-polyacrylamide gel, which provided a strong band of approximately 70 kDa. The purified enzyme also showed strong peptidase (gelatinase) activity (pH 7.2/40°C during zymography on 6-12% gelatin/1% gelatin-PAGE (at approx. 70 kDa). The CMCase activity is inhibited by the metal ions Mn/Cu/Fe/Co (50%), Hg/KMnO₄ (100%), and by glucose or lactose (50-75%; all at 1 mM). The observed dose/time-dependent inhibition by Hg ions could be prevented with 2-mercaptoethanol. A comparison of the B. agri endoglucanase-aminopeptidase (ELK43520; 350 aa) with other members of the M42-family revealed the conservation of active-site residues Cys256/Cys260, which were previously identified as metal-binding sites. Regulation of the endoglucanase activity probably occurs via metal binding-triggered changes in the redox state of the enzyme. Studies on this type of enzyme are of high importance for basic scientific and industrial research.

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Section: Discussionmentioning

confidence: 99%

The Dual Carboxymethyl Cellulase and Gelatinase Activities of a Newly Isolated Protein from Brevibacillus agri ST15c10 Confer Reciprocal Regulations in Substrate Utilization

Maiti

Samanta²,

Sahoo³

et al. 2017

Microb Physiol

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“…In our study, in the broth culture of purified bacterial species, Staphylococcus saprophyticus is Gram positive, cocci, and non-motile while the B. agri is Gram positive, rod shape, and highly motile. It has been reported that bacteria S. saprophyticus do not have flagella while in B. agri, it is present (Lara-Mayorga et al, 2010;Joshi et al, 2013). The presence of flagella in B. agri might play an important role in swarming property of parental isolated co-culture MS1-8, which might be supported by S. saprophyticus.…”

Section: Discussionmentioning

confidence: 93%

Molecular characterization of midgut bacteria of Aedes albopictus exhibiting swarming motility property

Yadav¹,

Naglot²,

Chandel³

et al. 2016

Afr. J. Microbiol. Res.

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Aedes mosquitoes are responsible for the transmission of arboviruses including dengue, chikungunya, zika, and yellow fever throughout the world. Mosquito gut harbors a variety of microbes, known to play a potential role in various physiological processes such as food digestion and pathogen development. Many bacterial species exert various movements like swarming, gliding and twitching which vary with individual bacterial species. Swarming is one of the movement patterns of bacterial cells in which newly growing cells direct themselves towards the edges of the colony on the solid agar plate. We have isolated a bacterial colony, which exerts swarming activity and moves away from its inoculation point following a translucent path. After repeated sub-culturing of the colony we isolated two different bacterial species which were found to be highly associated to each other in co-culture. Various techniques like biochemical analysis, 16S rRNA gene sequence based analysis, MALDI-TOFF MS and Scanning Electron Microscopy (SEM) were applied for in-depth characterization of isolates. The isolates were identified as Staphylococcus saprophyticus and Brevibacillus agri. Both bacterial isolates showed swarming activity in co-culture on the solid agar surface, but lost the activity when tested individually. Moreover, the swarming activity was even not recovered when both purified bacterial isolates were mixed at different concentrations. This demonstrated that an integral connection exists between the two species which is responsible for their swarming activity in co-culture.

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“…It is hypothesized that sulfate transporters and alkanesulfonate transporter may be responsible for organosulfur compound degradation (Erwin et al, 2005;Van Hamme et al, 2013). Moreover, a genome alignment of the only two sequenced B. agri genomes (B. agri 5-2 and B. agri BAB-2500 (Joshi et al, 2013)) showed that some functional regions are highly homologous between two assemblies. The alignment also reveals some discrepancies between them, some short stretches of the 5-2 genome absent from the contigs in BAB-2500 (Fig.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 98%

Genome sequence of Brevibacillus agri strain 5-2, isolated from the formation water of petroleum reservoir

She

Hang

et al. 2014

Marine Genomics

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Draft Genome Sequence of Brevibacillus sp. Strain BAB-2500, a Strain That Might Play an Important Role in Agriculture

Cited by 7 publications

References 16 publications

The Dual Carboxymethyl Cellulase and Gelatinase Activities of a Newly Isolated Protein from Brevibacillus agri ST15c10 Confer Reciprocal Regulations in Substrate Utilization

The Dual Carboxymethyl Cellulase and Gelatinase Activities of a Newly Isolated Protein from Brevibacillus agri ST15c10 Confer Reciprocal Regulations in Substrate Utilization

Molecular characterization of midgut bacteria of Aedes albopictus exhibiting swarming motility property

Genome sequence of Brevibacillus agri strain 5-2, isolated from the formation water of petroleum reservoir

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