Draft Genome Sequence of
            <i>Ignatzschineria</i>
            sp. Strain RMDPL8A, a Bacterium Isolated from Landfill Leachate

Nimonkar, Yogesh; Kajale, Swapnil; Dake, Manjusha; Prakash, Om

doi:10.1128/mra.00307-23

Cited by 1 publication

(5 citation statements)

References 15 publications

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“…The data available from other studies are limited to pH, TOC, BOD, and COD, and few more parameters and extensive data for comparison are lacking. Detection of the high levels of pollutants and heavy metals from this study supported previous data generated from landfill and landfill leachate in different studies conducted in the past ( Baldacci et al, 2018 ; Parvin and Tareq, 2021 ; Siddiqua et al, 2022 ; Nimonkar et al, 2023 ). Detection of various classes of contaminants from landfill and landfill leachate indicate that different kinds of mixed wastes discarded and dumped in municipal solid wastes, including industrial, hospital, pharmaceuticals, household, personal care products, electronics, batteries, ceramics, glass, paint, and agrochemicals.…”

Section: Discussionsupporting

confidence: 89%

“…We confirmed the positive growth of methanogens in enrichments by confirmation of methane accumulation in the headspace of enrichment vials using gas chromatography (GC), increased cell turbidity of culture medium, and detection of fluorescence in enriched cells under the microscope due to the presence of cofactor F 420 in methanogens ( Nimonkar et al, 2023 ). Although we successfully enriched the methanogens from all enrichments ( Supplementary Figure 3 ) except Methanosarcina , we could not cultivate other methanogens in pure culture.…”

Section: Resultsmentioning

confidence: 58%

“…After every 2 weeks, 5 ml of culture was anaerobically transferred into a fresh medium supplemented with vancomycin to suppress bacterial growth (Hilpert et al, 1981). Positive enrichment or growth of methanogens in serum vials was confirmed by detection of methane formation in headspace using gas chromatography (GC), increase in optical density of the culture medium, flame test (burning) of headspace gas, and detection of cell fluorescence at 420 nm using fluorescence microscopy as discussed by Nimonkar et al (2023).…”

Section: Enrichment Of Methanogenic Archaeamentioning

confidence: 99%

“…Due to population growth, urbanization, industrialization, and economic development, managing municipal solid waste is an emerging challenge for municipal corporations ( Rawat and Ramanathan, 2011 ; Ajay et al, 2022 ; Fazzo et al, 2023 ). Landfilling is a cheap municipal solid waste management method in developing nations ( Nimonkar et al, 2023 ). Landfills act as anaerobic digesters where sequential microbiological processes such as hydrolysis, acidogenesis, acetogenesis, and methanogenesis decompose the biodegradable waste and produce greenhouse gases (CH 4 , CO 2 , and N 2 O) and other toxins and pollutants.…”

Section: Introductionmentioning

confidence: 99%

“…In India, landfilling is one of the preferred sources of municipal solid waste (MSW) management ( Sagar et al, 2022 ; Nimonkar et al, 2023 ). Although the percentage of sanitary landfills is increasing, to mitigate climate change and public health issues, some landfills still need to be adequately managed ( Nair, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Culture-based and culture-independent approach for the study of the methanogens and obligate anaerobes from different landfill sites

Prakash,

Dewala,

Nimonkar

et al. 2024

Front. Microbiol.

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The landfill is a cheap way of solid waste management in developing countries. The majority of landfills are non-sanitary and work as open garbage dumping sites and pose threats to public and environmental health. Therefore, an in-depth understanding of the chemistry and microbiology of landfills is imperative to develop the right policies for landfill management. In the current study, we investigated the chemistry and microbiology of three Indian landfill sites using culture-based and culture-independent molecular approaches. Our data indicate that the nature of landfills varies from site to site in terms of chemistry, pollutants, and pathogens. We also enriched and cultivated three methanogens using an optimized medium and constructed two high-quality draft genomes from enriched microbiomes using metagenome-assembled genome approaches. The phylogenomic study of one draft genome showed the highest 93% sequence similarity with members of Methanomassiliicoccaceae and was always enriched with Acholoplasma and Anaerohalosphaera lusitana. Despite all the efforts, we did not isolate it in pure culture and hypothesized that for the cultivation of some not-yet-cultured methanogen, the presence of other organisms plays an important role, and their syntrophic interaction must be discerned for its successful cultivation in the future. Co-cultivation of amino acid-degrading organisms indicates that their co-culture can assist in boosting the growth of methanogens. In addition, our data indicated that landfill leachate contains a heavy load of pollutants and treatment is a must before discharge in nature or use in irrigation or biofertilizer.

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Section: Discussionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 58%