Draft Genome Sequence of
            <i>Lactobacillus helveticus</i>
            ATCC 12046

Palomino, María Mercedes; Burguener, Germán; Campos, Josefina; Allievi, Mariana C.; Fina-Martin, Joaquina; Acosta, Mariano; Porto, Darío Augusto Fernández Do; Ruzal, Sandra M.

doi:10.1128/genomea.01595-17

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…PRJNA78291. To enrich functional annotations and increase the quality of the resulting metabolic network, an annotation pipeline based on the standard operating procedures (SOPs) of the Integrative Services for Genomic Analysis (ISGA) ( Hemmerich et al., 2010 ) was performed as previously reported ( Burguener et al., 2014 ; Palomino et al., 2018 ). The Genbank annotation file was expanded with Enzyme Commission (EC) annotations, which increased the accuracy of the gene-protein-reaction associations inferred by Pathway Tools ( Karp et al., 2011 ).…”

Section: Methodsmentioning

confidence: 99%

Pathway Driven Target Selection in Klebsiella pneumoniae: Insights Into Carbapenem Exposure

Serral

Pardo

Sosa

et al. 2022

Front. Cell. Infect. Microbiol.

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Carbapenem-resistant Klebsiella pneumoniae (CR-KP) represents an emerging threat to public health. CR-KP infections result in elevated morbidity and mortality. This fact, coupled with their global dissemination and increasingly limited number of therapeutic options, highlights the urgency of novel antimicrobials. Innovative strategies linking genome-wide interrogation with multi-layered metabolic data integration can accelerate the early steps of drug development, particularly target selection. Using the BioCyc ontology, we generated and manually refined a metabolic network for a CR-KP, K. pneumoniae Kp13. Converted into a reaction graph, we conducted topological-based analyses in this network to prioritize pathways exhibiting druggable features and fragile metabolic points likely exploitable to develop novel antimicrobials. Our results point to the aptness of previously recognized pathways, such as lipopolysaccharide and peptidoglycan synthesis, and casts light on the possibility of targeting less explored cellular functions. These functions include the production of lipoate, trehalose, glycine betaine, and flavin, as well as the salvaging of methionine. Energy metabolism pathways emerged as attractive targets in the context of carbapenem exposure, targeted either alone or in conjunction with current therapeutic options. These results prompt further experimental investigation aimed at controlling this highly relevant pathogen.

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