Draft Genome Sequence of
            <i>Lysinibacillus fusiformis</i>
            Strain Juneja, a Laboratory-Derived Pathogen of
            <i>Drosophila melanogaster</i>

Smith, Brittny R.; Unckless, Robert L.

doi:10.1128/genomea.01571-17

Cited by 7 publications

(9 citation statements)

References 7 publications

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“…fusiformis strains have a low entomopathogenic effect against Spodoptera exigua Hübner (Lepidoptera: Noctuidae) [ 84 ]. However, other strains of this species are highly lethal to Drosophila melanogaster and show miticidal effect against Varroa destructor [ 85 , 86 ]. These previous literature reports together with our results indicate a great entomopathogenic potential of L .…”

Section: Discussionmentioning

confidence: 99%

Entomopathogenic potential of bacteria associated with soil-borne nematodes and insect immune responses to their infection

et al. 2023

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Soil-borne nematodes establish close associations with several bacterial species. Whether they confer benefits to their hosts has been investigated in only a few nematode-bacteria systems. Their ecological function, therefore, remains poorly understood. In this study, we isolated several bacterial species from rhabditid nematodes, molecularly identified them, evaluated their entomopathogenic potential on Galleria mellonella larvae, and measured immune responses of G. mellonella larvae to their infection. Bacteria were isolated from Acrobeloides sp., A. bodenheimeri, Heterorhabditis bacteriophora, Oscheius tipulae, and Pristionchus maupasi nematodes. They were identified as Acinetobacter sp., Alcaligenes sp., Bacillus cereus, Enterobacter sp., Kaistia sp., Lysinibacillus fusiformis, Morganella morganii subsp. morganii, Klebsiella quasipneumoniae subsp. quasipneumoniae, and Pseudomonas aeruginosa. All bacterial strains were found to be highly entomopathogenic as they killed at least 53.33% G. mellonella larvae within 72h post-infection, at a dose of 106 CFU/larvae. Among them, Lysinibacillus fusiformis, Enterobacter sp., Acinetobacter sp., and K. quasipneumoniae subsp. quasipneumoniae were the most entomopathogenic bacteria. Insects strongly responded to bacterial infection. However, their responses were apparently little effective to counteract bacterial infection. Our study, therefore, shows that bacteria associated with soil-borne nematodes have entomopathogenic capacities. From an applied perspective, our study motivates more research to determine the potential of these bacterial strains as biocontrol agents in environmentally friendly and sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Entomopathogenic potential of bacteria associated with soil-borne nematodes and insect immune responses to their infection

et al. 2023

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“…Lysinibacillus fusiformis (strain Juneja) is a natural pathogen of Drosophila obtained from Brian Lazzaro at Cornell University [45]. Bacteria were grown from single colonies in LB liquid media overnight to stationary phase, then concentrated to an optical density (600nm) of about 4.0.…”

Section: Methodsmentioning

confidence: 99%

“…Our study investigated natural variation in defense against the Gram-positive Lysinibacillus fusiformis , which was isolated from Drosophila in the lab [45]. We measured survival 2- and 5-days after septic injury with L. fusiformis in the DGRP population and both DSPR populations (A and B).…”

Section: Introductionmentioning

confidence: 99%

The genetic basis of variation immune defense againstLysinibacillus fusiformisinfection inDrosophila melanogaster

Smith

Patch

Unckless

2022

Preprint

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The genetic causes of phenotypic variation often differ depending on the population examined, particularly if the populations were founded by relatively small numbers of genotypes. Similarly, the genetic causes of phenotypic variation among similar traits (resistance to different xenobiotic compounds or pathogens) may also be completely different or only partially overlapping. Differences in genetic causes for variation in the same trait among populations suggests considerable context dependence for how selection might act on those traits. Similarities in the genetic causes of variation for different traits, on the other hand, suggests pleiotropy which also would influence how natural selection would shape variation in a trait. We characterized immune defense against a naturalDrosophilapathogen, the Gram-positiveLysinibacillus fusiformis, in three different populations and found almost no overlap in the genetic architecture of variation in survival post infection. However, when comparing our results to a similar experiment with the fungal pathogen,B. bassiana, we found a convincing shared QTL peak for both pathogens. This peak contains theBomanincluster ofDrosophilaimmune effectors. RNAi knockdown experiments confirms a role of some of these genes in immune defense against both pathogens. This suggests that natural selection may act on the entire cluster ofBomaningenes (and the linked region under the QTL) or specific peptides for specific pathogens.Author SummaryLike most traits, the way individuals respond to infection vary among individuals within a population. Some of this variation is caused by genetic differences in the host organism. Over the past decade, two prominent resources were developed to assess genetic variation for complex traits of the fruit fly,Drosophila melanogasterand map the genetic variants responsible. We recently described a strain ofLysinibacillus fusiformisbacteria, which was isolated from fruit flies and is moderately virulent when flies are infected. We mapped genetic variation in resistanceL. fusiformisusing these mapping resources. We find that among the resources, different changes were associated with immune defense. However, we also found that within a resource, the same region of the genome was associated with resistance to bothL. fusiformisand a fungal pathogen. These results suggest that different populations adapt differently to the same pathogens, but two distinct pathogens share similar causes of genetic variation within a single population.

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“…Providencia burhodegraneria Strain B [80], Enterococcus faecalis [80]), Serratia marcescens [39], Lysinibacillus fusiformis Strain Juneja [81], and Staphylococcus succinus (isolated from wild Drosophila, Unckless lab). Bacteria were grown from glycerol stocks on LB plates at 37°C overnight.…”

Section: Bacterial Strainsmentioning

confidence: 99%

A suite of selective pressures supports the maintenance of alleles of aDrosophilaimmune peptide

Mullinax,

Darby,

Gupta

et al. 2023

Preprint

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The innate immune system provides hosts with a crucial first line of defense against pathogens. While immune genes are often among the fastest evolving genes in the genome, in Drosophila, antimicrobial peptides (AMPs) are notable exceptions. Instead, AMPs may be under balancing selection, such that over evolutionary timescales multiple alleles are maintained in populations. In this study, we focus on the Drosophila antimicrobial peptide Diptericin A, which has a segregating amino acid polymorphism associated with differential survival after infection with the Gram-negative bacteria Providencia rettgeri. Diptericin A also helps control opportunistic gut infections by common Drosophila gut microbes, especially those of Lactobacillus plantarum. In addition to genotypic effects on gut immunity, we also see strong sex-specific effects that are most prominent in flies without functional diptericin A. To further characterize differences in microbiomes between different diptericin genotypes, we used 16S metagenomics to look at the microbiome composition. We used both lab reared and wild caught flies for our sequencing and looked at overall composition as well as the differential abundance of individual bacterial families. Overall, we find flies that are homozygous serine for diptericin A are better equipped to survive a systemic infection from P. rettgeri, but in general homozygous arginine flies have a longer lifespan after being fed common gut commensals. Our results suggest a possible mechanism for the maintenance of genetic variation of diptericin A through the complex interactions of sex, systemic immunity, and the maintenance of the gut microbiome.

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Draft Genome Sequence of Lysinibacillus fusiformis Strain Juneja, a Laboratory-Derived Pathogen of Drosophila melanogaster

Cited by 7 publications

References 7 publications

Entomopathogenic potential of bacteria associated with soil-borne nematodes and insect immune responses to their infection

Entomopathogenic potential of bacteria associated with soil-borne nematodes and insect immune responses to their infection

The genetic basis of variation immune defense againstLysinibacillus fusiformisinfection inDrosophila melanogaster

A suite of selective pressures supports the maintenance of alleles of aDrosophilaimmune peptide

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