Draft Genome Sequence of
            <i>Streptomyces</i>
            sp. SPMA113, a Prajinamide Producer

The cyclic lipopeptide antibiotics structurally-related to daptomycin were first reported in the 1950s. Several have common lipopeptide initiation, elongation, and termination mechanisms. Initiation requires the use of a fatty-acyl AMP ligase (FAAL), a free-standing acyl carrier protein (ACP), and a specialized condensation (CIII) domain on the first NRPS elongation module to couple the long chain fatty acid to the first amino acid. Termination is carried out by dimodular NRPS that contains a terminal thioesterase (Te) domain (CAT-CATTe). Lipopeptide BGCs also encode ABC transporters, apparently for export and resistance. The use of this mechanism of initiation, elongation, and termination, coupled with molecular target-agnostic resistance, has provided a unique basis for robust natural and experimental combinatorial biosynthesis to generate a large variety of structurally-related compounds, some with altered or different antibacterial mechanisms of action. The FAAL, ACP, and dimodular NRPS genes were used as molecular beacons to identify phylogenetically-related BGCs by BLASTp analysis of finished and draft genome sequences. These and other molecular beacons have identified: (i) known, but previously unsequenced lipopeptide BGCs in draft genomes; (ii) a new daptomycin family BGC in a draft genome of Streptomyces sedi; and (iii) novel lipopeptide BGCs in the finished genome of Streptomyces ambofaciens and the draft genome of Streptomyces zhaozhouensis.

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Genome Mining for Actinomycete Biosynthetic Gene Clusters

Dudbridge¹

2018

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Draft Genome Sequence of Streptomyces sp. SPMA113, a Prajinamide Producer

Cited by 3 publications

References 14 publications

Development of a drug discovery approach from microbes with a special focus on isolation sources and taxonomy

Development of a drug discovery approach from microbes with a special focus on isolation sources and taxonomy

Genome mining for drug discovery: cyclic lipopeptides related to daptomycin

Genome Mining for Actinomycete Biosynthetic Gene Clusters

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