Draft Genome Sequence of
            <i>Trichomonas tenax</i>
            Strain Hs-4:NIH

Yang, Nawu; Christie, Jevan; Keen, Henry L.; Matthew, Maurice A.; Yao, Chaoqun

doi:10.1128/mra.00157-22

Cited by 2 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… * Benabdelkader et al ( 2019 ) and Yang et al ( 2022 ). ** BUSCO score based on the integrated annotations (20,287 proteins) from the 2019 and 2022 draft genomes.…”

Section: Resultsmentioning

confidence: 98%

“…GenSAS (Humann et al, 2019 ) was used to annotate the two draft genome sequences of T. tenax strain Hs-4:NIH (NCBI Genome assemblies PRJEB22701 and ASM2309173v1) (Benabdelkader et al, 2019 ; Yang et al, 2022 ). Identification and masking of repeats was achieved using RepeatMasker (Nishimura, 2000 ) and RepeatModeler (Smit and Hubley, 2008 ) on GenSAS.…”

Section: Methodsmentioning

confidence: 99%

“…Benabdelkader et al ( 2019 ) conducted this study to investigate the prevalence and genetic diversity of T. tenax and its potential involvement in the severity of periodontitis among humans. A second draft whole genome sequence dataset for the same strain of T. tenax was published in 2022 (63.4 Mb, across 4906 contigs) (Yang et al, 2022 ). Both draft genomes are smaller than the 133 ± 4 Mb estimated from flow cytometry (Zubáčová et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

“…The two published T. tenax draft genomes did not include annotation of protein-coding genes, which is essential to guide the study of the molecular cell biology of a given organism. Here, we investigated T. tenax protein-coding genes by integrating and annotating the two published draft genomes (Benabdelkader et al, 2019 ; Yang et al, 2022 ). The predicted T. tenax protein-coding genes and their functional annotations were compared with the predicted proteome of a new assembly of T. vaginalis .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative genomics between Trichomonas tenax and Trichomonas vaginalis: CAZymes and candidate virulence factors

Mpeyako,

Hart,

Bailey

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionThe oral trichomonad Trichomonas tenax is increasingly appreciated as a likely contributor to periodontitis, a chronic inflammatory disease induced by dysbiotic microbiota, in humans and domestic animals and is strongly associated with its worst prognosis. Our current understanding of the molecular basis of T. tenax interactions with host cells and the microbiota of the oral cavity are still rather limited. One laboratory strain of T. tenax (Hs-4:NIH/ATCC 30207) can be grown axenically and two draft genome assemblies have been published for that strain, although the structural and functional annotation of these genomes is not available.MethodsGenSAS and Galaxy were used to annotate two publicly available draft genomes for T. tenax, with a focus on protein-coding genes. A custom pipeline was used to annotate the CAZymes for T. tenax and the human sexually transmitted parasite Trichomonas vaginalis, the most well-characterized trichomonad. A combination of bioinformatics analyses was used to screen for homologs of T. vaginalis virulence and colonization factors within the T. tenax annotated proteins.ResultsOur annotation of the two T. tenax draft genome sequences and their comparison with T. vaginalis proteins provide evidence for several candidate virulence factors. These include candidate surface proteins, secreted proteins and enzymes mediating potential interactions with host cells and/or members of the oral microbiota. The CAZymes annotation identified a broad range of glycoside hydrolase (GH) families, with the majority of these being shared between the two Trichomonas species.DiscussionThe presence of candidate T. tenax virulence genes supports the hypothesis that this species is associated with periodontitis through direct and indirect mechanisms. Notably, several GH proteins could represent potential new virulence factors for both Trichomonas species. These data support a model where T. tenax interactions with host cells and members of the oral microbiota could synergistically contribute to the damaging inflammation characteristic of periodontitis, supporting a causal link between T. tenax and periodontitis.

show abstract

“… * Benabdelkader et al ( 2019 ) and Yang et al ( 2022 ). ** BUSCO score based on the integrated annotations (20,287 proteins) from the 2019 and 2022 draft genomes.…”

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative genomics between Trichomonas tenax and Trichomonas vaginalis: CAZymes and candidate virulence factors

Mpeyako,

Hart,

Bailey

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The area of exosome studies has shown great potential in finding the pathogenicity of various eukaryotic cells. With this new area of proteomics and the recent publication of the T. tenax draft genome [ 93 ], we can possibly move closer to mapping the pathway of T. tenax and developing a possible drug therapy that can control and prevent the transmission of this oral flagellate.…”

Section: Discussionmentioning

confidence: 99%

Trichomonas tenax: A Neglected Protozoan Infection in the Oral Cavities of Humans and Dogs—A Scoping Review

et al. 2023

Self Cite

View full text Add to dashboard Cite

Trichomonas tenax is a flagellated protozoan parasite found in the oral cavities of humans and animals and has been associated with periodontal disease, the most prevalent inflammatory disease affecting them all. Studies have shown that T. tenax can cause damage to mammalian cells and secretes virulent proteins, such as cysteine. It is presently considered zoonotic. Despite the few studies that have been done, the pathogenicity of this oral protozoan is still not fully understood. A database search was performed in July 2022 using PubMed and Google Scholar to retrieve data eligible for this study. PRISMA-ScR guidelines were followed to conduct this scoping review. A total of 321 articles were found with 87 included in this review after applying the exclusion criteria. Due to its increasing prevalence worldwide in both humans and dogs, detecting and elucidating the pathogenicity of this parasite is paramount for effective global control and prevention of periodontal disease. However, there is a paucity in the literature on this neglected zoonotic trichomonad, which is in large contrast to the closely related human pathogen T. vaginalis. Here, we comprehensively review the history, morphology and reproduction, host, prevalence, diagnosis, pathogenicity, control, and prevention of T. tenax. Hopefully, this article will call attention to both medical and veterinary professionals as well as epidemiologists on this most neglected and zoonotic protozoan. More epidemiological and clinical studies need to be conducted on T. tenax to gain a better understanding of its pathogenicity, to increase the chances of developing effective drugs to aid in the control of this oral parasite, and reduce the spread of periodontal disease worldwide.

show abstract

Draft Genome Sequence of Trichomonas tenax Strain Hs-4:NIH

Cited by 2 publications

References 13 publications

Comparative genomics between Trichomonas tenax and Trichomonas vaginalis: CAZymes and candidate virulence factors

Comparative genomics between Trichomonas tenax and Trichomonas vaginalis: CAZymes and candidate virulence factors

Trichomonas tenax: A Neglected Protozoan Infection in the Oral Cavities of Humans and Dogs—A Scoping Review

Contact Info

Product

Resources

About