Draft Genome Sequence of Lactobacillus crispatus CIP 104459, Isolated from a Vaginal Swab

Abstract

We report the draft genome sequence of Lactobacillus crispatus CIP 104459, isolated from a human vaginal swab. This draft genome consists of 1,993,673 bp, with 36.8% G+C content and 2,024 predicted protein-encoding sequences.

Effect of 17β-estradiol on a Human Vaginal Lactobacillus Crispatus Strain

“…As shown in this study, 17β-estradiol displayed effects on the physiology of L. crispatus CIP104459 at concentrations as low as 10 -8 or 10 -10 M for some phenotypes, suggesting the existence of a putative sensor for estradiol in this bacterium. The potential 17β-estradiol binding site was investigated using a bioinformatic approach based on the recently published draft genome of L. crispatus CIP104459 28 that was compared to the recently annotated genome of L. crispatus CO3MRSI1 34 . The putative translated ORF were then compared to the published sequence of nine eukaryotic 17β-estradiol binding proteins that were previously identi ed in the literature ( Table 2).…”

“…In order to identify the potential 17β-estradiol binding protein in L. crispatus CIP104459, we used a bioinformatic approach based on its recently published draft genome 28 . For reference, we selected a series of nine molecules identi ed as estradiol binding proteins in eukaryotes.…”

“…In our laboratory, this strain was stored at -140°C in a cryofreezer (Thermo Fisher Scienti c, Waltham, Massachusetts, USA). The draft genome sequence of L. crispatus CIP 104459 was recently determined and deposited in the DDBJ/ENA/GenBank under accession number VOMA00000000 28 . This bacterium was grown anaerobically in De Man, Rogosa and Sharpe (MRS) medium (VWR, Fontenay-sous-Bois, France) at 37°C under static conditions into conical 15 ml tubes (Corning®, Thermo Fisher Scienti c, Waltham, Massachusetts, USA) lled with medium to maximal capacity.…”

“…Then we decided to use bioinformatics approaches to investigate potential 17β-estradiol binding site(s) in L. crispatus. As previously mentioned, the draft genome of L. crispatus CIP104459 was recently published 28 . This draft genome was aligned by BLASTp on ExPASy (https:// web.expasy.org/blast) with the annotated sequence of L. crispatus CO3MRSI1 34 to improve its de nition.…”

“…In the present study, the effects of estradiol on a vaginal strain of L. crispatus, whose genome was recently sequenced 28 , was investigated through physiological, physicochemical, morphological, cell binding assay and bioinformatics approaches. Our results demonstrate that estradiol can affect the Lactobacilli physiology, suggesting that estradiol could play a key role in their implantation onto the vaginal mucosa.…”