Draft Genome Sequence of <b><i>Bacillus cereus</i></b> CITVM-11.1, a Strain Exhibiting Interesting Antifungal Activities

Abstract

Bacillus cereus is a gram-positive, spore-forming bacterium possessing an important and historical record as a human-pathogenic bacterium. However, several strains of this species exhibit interesting potential to be used as plant growth-promoting rhizobacteria. Here, we report the draft genome sequence of B. cereus strain CITVM-11.1, which consists of 37 contig sequences, accounting for 5,746,486 bp (with a GC content of 34.8%) and 5,752 predicted protein-coding sequences. Several of them could potentially be involved in plant-bacterium interactions and may contribute to the strong antagonistic activity shown by this strain against the charcoal root rot fungus, Macrophomina phaseolina. This genomic sequence also showed a number of genes that may confer this strain resistance against several polluting heavy metals and for the bioconversion of mycotoxins.

Genomic and metabolic features of Bacillus cereus, inhibiting the growth of Sclerotinia sclerotiorum by synthesizing secondary metabolites

“…Genome analysis revealed many gene clusters involved in the synthesis of antifungal agents (Kamada et al 2014). Draft genome sequence of B. cereus strain CITVM-11.1, including 5752 predicted protein-coding sequences some of which were involved in plant-bacteria interactions and contribute to the strong antagonistic activity against the charcoal root rot pathogen, Macrophomina phaseolina (Caballero et al 2018). Bacillus cereus E41 genome contains a complete gene cluster for the lantibiotic thusin (thsA1TM1A2A2 = M2FE), which is predicted to produce the siderophore petrobactin (asbABC-DEF) (Daas et al 2017).…”

Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests

“…Total DNA was electrophoresed in 1% agarose gels stained with SYBR Safe (Thermo Fisher Scientific) and quantified using PICODROP (PICO 100 µl Spectrophotometer). The purified DNA was then used to construct a pooled Illumina library and sequenced at Stabvida (Portugal) by using high-throughput Illumina sequencing technology with a genomic coverage of 1000´ (Caballero et al, 2018).…”

“…The raw Illumina reads obtained were first trimmed and assembled into contigs by using Geneious R11 (www.geneious.com), with the de novo assembly tool and default parameters. The resultant contigs were then analysed with BLAST (Altschul et al, 1990) using a customized non-redundant insecticidal protein database (Caballero et al, 2018). Genome annotation was performed with the NCBI Prokaryotic Genome Annotation Pipeline (2018 release) although it was also annotated with RAST (Aziz et al, 2008).…”

Genome sequence analysis and insecticidal characterization of &lt;em&gt;Bacillus thuringiensis&lt;/em&gt; Bt-UNVM_94, a strain showing dual insecticidal activity against lepidopteran and coleopteran pests.

“…Total DNA isolation (chromosome and plasmids) was performed using the Wizard genomic DNA purification kit (Promega) and purified DNA sequenced at Stabvida (Portugal) by using high-throughput Illumina sequencing technology with a genomic coverage of 1000  [23]. The raw Illumina reads obtained were first trimmed and assembled in contigs by using Geneious R11 (www.geneious.com), with the de novo assembly tool and default parameters.…”