Draft genome sequence of Phyllosticta ampelicida, the cause of grapevine black rot

Abstract

Phyllosticta ampelicida causes grapevine black rot, a potentially damaging disease for grape production. This paper reports the draft genome sequence of P. ampelicida PA1 Galicia CBS 148563, which is 30.55 Mb and encodes 10,691 predicted protein-coding genes. This is the first sequence genome assembly of P. ampelicida, and this information is a valuable resource to support genomic attributes for determining pathogenic behaviour and comparative genomic analyses of grapevine black rot fungi.

Fungal trunk diseases of fruit trees in Europe: pathogens, spread and future directions

“…HTS methods are probably the most significant advances in molecular biology since the advent of the PCR process. Microbial/fungal genome sequencing or metagenomics/ metabarcoding become a routine analysis and using the latest technology it is possible to generate near complete genomes (Robert-Siegwald et al, 2017;Eichmeier et al, 2022) or whole spectra of microbial/fungal communities (Eichmeier et al, 2018;Bruez et al, 2020;Gramaje et al 2022). There are also other applications of HTS technology which can serve understanding of the plant pathogen interaction as transcriptomics (Romeo-Oliván et al, 2022) or small RNA sequencing (Eichmeier et al, 2019).…”

Genome analysis of the esca-associated Basidiomycetes Fomitiporia mediterranea, Fomitiporia polymorpha, Inonotus vitis, and Tropicoporus texanus reveals virulence factor repertoires characteristic of white-rot fungi

“…The majority of wood-colonizing species that cause grapevine trunk diseases belong to the Ascomycota phylum, with only a few Basidiomycetes species confirmed as pathogens primarily of esca (Del Frari et al 2021;Fischer and González García 2015;Brown et al 2020;Fischer 2000Fischer , 2002Martín et al 2022). Whereas the primary pathogens of esca were long thought to be Ascomycetes Phaeomoniella chlamydospora and Phaeoacremonium minimum, various members of the Hymenochaetaceae family, including species like Fomitiporia mediterranea and Inonotus spp., have been isolated in esca-symptomatic vines in vineyards around the world (Fischer and González García 2015;Brown et al 2020;Fischer 2002;Guerin-Dubrana et al 2019;Pacetti et al 2022;Mundy et al 2020;Cloete et al 2015bCloete et al , 2015aMoretti et al 2021). Brown et al (Brown et al 2020) isolated multiple Basidiomycete species from vineyards with esca in California and Texas, and described novel species Fomitiporia ignea, Inonotus vitis, and Tropicoporus texanus.…”