Draft genome sequence of plastic degrading Bacillus sp. AIIW2 isolated from the Arabian ocean

Abstract

The endemic spread of plastic in the environment requires urgent need of a sustainable approach. Marine microbes found to have vast bioactivity and play a central role in biogeochemical cycling in the ocean; however, very few of them had been explored for biochemical cycling or plastic degradation. In the present study, we report the draft genome sequence of marine Bacillus sp. AIIW2 which was found to utilize plastic as a carbon source. The Bacillus sonorensis SRCM101395 was used as a reference genome for mapping the reads. The genome size of strain AIIW2 was approximately 4.4 Mb and composed of 4737 coding sequences with 45.7% G + C contents. The whole genome comparison of strain AIIW2 with three closest Bacillus strains showed strain specificity, the 16S ribosomal RNA sequence shows 99.93% similarity with Bacillus paralicheniformis KJ‐16T (KY694465). This genome data would provide the genetic basis in developing plastic bioremediation approaches and discover the enzymes pertinent in the biodegradation processes.

Transcriptome-Guided Insights Into Plastic Degradation by the Marine Bacterium

“…Misassembled transcripts from RNA-seq were filtered out with the help of genome sequence data of Bacillus species AIIW2 (KY694465) ( Kumari et al, 2020 ). A total of 63,27,406 and 56,70,745 high-quality raw reads were generated out of three replicates of transcriptome sequencing data of control and treated samples, respectively.…”