Draft Genome Sequence of Pseudomonas putida Strain GM4FR, an Endophytic Bacterium Isolated from Festuca rubra L

Wemheuer, Franziska; Hollensteiner, Jacqueline; Granzow, Sandra; Daniel, Rolf; Vidal, Stefan; Wemheuer, Bernd

doi:10.1128/genomea.00086-17

Cited by 4 publications

(1 citation statement)

References 20 publications

(21 reference statements)

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“…These values are higher than the average of clusters found in the genomes of other Pseudomonas strains isolated from different origins. For example, recently published genomes of Pseudomonas strains isolated from soil or rhizosphere encode 9 (Hennessy et al, 2015 ), 13 (Vida et al, 2017 ) or 16 (Adam et al, 2015 ) of these clusters; in the same way, Pseudomonas strains isolated from plant tissues present 4 (Wemheuer et al, 2017 ) or 6 (Maggini et al, 2017 ) of these clusters. Most of these new genomes have revealed new potentially bioactive compounds in this genus, but importantly, many of the substances predicted in the genome sequences of the strains of this study have never been identified in other Pseudomonas strains.…”

Section: Discussionmentioning

confidence: 98%

Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.

et al. 2018

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Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments for microbial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs. Bark beetles live in phloem of host trees and establish symbioses with microorganisms that provide them with nutrients. In addition, some of their associated bacteria play a role in the beetle protection by producing substances that inhibit antagonists. In this study the capacity of several bacterial strains, isolated from the bark beetles Ips acuminatus, Pityophthorus pityographus Cryphalus piceae, and Pityogenes bidentatus, to produce antimicrobial compounds was analyzed. Several isolates exhibited the capacity to inhibit Gram-positive and Gram-negative bacteria, as well as fungi. The genome sequence analysis of three Pseudomonas isolates predicted the presence of several gene clusters implicated in the production of already described antimicrobials and moreover, the low similarity of some of these clusters with those previously described, suggests that they encode new undescribed substances, which may be useful for developing new antimicrobial agents. Moreover, these bacteria appear to have genetic machinery for producing antitumoral and antiviral substances. Finally, the strain IA19T showed to represent a new species of the genus Pseudomonas. The 16S rRNA gene sequence analysis showed that its most closely related species include Pseudomonas lutea, Pseudomonas graminis, Pseudomonas abietaniphila and Pseudomonas alkylphenolica, with 98.6, 98.5 98.4, and 98.4% identity, respectively. MLSA of the housekeeping genes gyrB, rpoB, and rpoD confirmed that strain IA19T clearly separates from its closest related species. Average nucleotide identity between strains IA19T and P. abietaniphila ATCC 700689T, P. graminis DSM 11363T, P. alkylphenolica KL28T and P. lutea DSM 17257T were 85.3, 80.2, 79.0, and 72.1%, respectively. Growth occurs at 4-37°C and pH 6.5-8. Optimal growth occurs at 28°C, pH 7–8 and up to 2.5% NaCl. Respiratory ubiquinones are Q9 (97%) and Q8 (3%). C16:0 and in summed feature 3 are the main fatty acids. Based on genotypic, phenotypic and chemotaxonomic characteristics, the description of Pseudomonas bohemica sp. nov. has been proposed. The type strain is IA19T (=CECT 9403T = LMG 30182T).

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Section: Discussionmentioning

confidence: 98%

Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.

et al. 2018

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The Genomes of Endophytic Bacteria

Frank

2018

Endophytes of Forest Trees

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Antibiotic- and metal-resistant endophytes inhabit Armeria maritima hyperaccumulator

Goryluk-Salmonowicz,

Myczka,

Popowska

2023

Plant Soil

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Background and aims Recent research has recognized the presence of metal-resistant bacteria in plants and their role in phytoremediation intensification. However, information on the antibiotic resistance profile of those bacteria remains scarce. This study, describes the first isolation of endophytic bacteria from green parts of Armeria maritima growing on mine-tailing soil in southern Poland, and presents the resistance profile of these microorganisms. Methods Bacteria were isolated from internal tissues of Armeria maritima and characterized. Minimal Inhibitory Concentration (MIC) of metals was determined by the plate dilution method using (CH3COO)2Pb and ZnSO4 supplemented medium; antibiotic susceptibility was determined by disk diffusion method according to EUCAST version 11.0; the whole genome sequencing was performed using the MiSeq platform (Illumina). The physicochemical properties of soil were evaluated according to European Standards. Results Toxic metal-resistant bacteria were isolated from the green parts of Armeria maritima. The endophytes were identified as Pseudomonas spp. The annotated bacterial genomes carried genes encoding numerous metal ion transporters, metal reducing enzymes and efflux pump components. The bacteria were resistant to streptomycin, fosfomycin and ß-lactams. Moreover, genome analysis revealed the presence of MacAB-TolC efflux pump genes conferring resistance to macrolides, the multidrug efflux pumps AcrAB-TolC and MexAB-OprM. Conclusion Armeria maritima is inhabited by endophytic bacteria identified as Pseudomonas species that are resistant to metals and to antibiotics. Under the One Health concept the contamination of soil and plants with ARB and ARGs should be monitored and limited and a regulatory framework for safety use of bacterial bioinoculants should be established.

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Draft Genome Sequence of Pseudomonas putida Strain GM4FR, an Endophytic Bacterium Isolated from Festuca rubra L

Cited by 4 publications

References 20 publications

Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.

Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.

The Genomes of Endophytic Bacteria

Antibiotic- and metal-resistant endophytes inhabit Armeria maritima hyperaccumulator

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