Draft Genome Sequence of Pseudomonas sp. Strain Ag1, Isolated from the Midgut of the Malaria Mosquito Anopheles gambiae

Alvarez, Celeste; Kukutla, Phanidhar; Jiang, Jinjin; Yu, Wanqin; Xu, Jianchu

doi:10.1128/jb.01173-12

Cited by 17 publications

(21 citation statements)

References 13 publications

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“…This is due to genome rearrangement, probably caused by IS3-family transposases (PSF113_1803-1804 and PSF113_4042-4043). Examination of other P. fluorescens genomes indicated that the genes invG , iagB and pseudogene related to hilA are present at the same chromosomal location in strains PAMC 25886 [66], Ag1 [67], 14–3 [68], WH6 [69], SBW25 [18], R81 [70], A506, BG33R, SS101 [20], NZ007, NZ052 and PMS117. Interestingly all these strains belong to subgroup V of the P. fluorescens group, which suggest that the SPI-1 cluster was lost before speciation of this sub-clade.…”

Section: Resultsmentioning

confidence: 99%

Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction

et al. 2013

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BackgroundPseudomonas fluorescens F113 is a plant growth-promoting rhizobacterium (PGPR) isolated from the sugar-beet rhizosphere. This bacterium has been extensively studied as a model strain for genetic regulation of secondary metabolite production in P. fluorescens, as a candidate biocontrol agent against phytopathogens, and as a heterologous host for expression of genes with biotechnological application. The F113 genome sequence and annotation has been recently reported.ResultsComparative analysis of 50 genome sequences of strains belonging to the P. fluorescens group has revealed the existence of five distinct subgroups. F113 belongs to subgroup I, which is mostly composed of strains classified as P. brassicacearum. The core genome of these five strains is highly conserved and represents approximately 76% of the protein-coding genes in any given genome. Despite this strong conservation, F113 also contains a large number of unique protein-coding genes that encode traits potentially involved in the rhizocompetence of this strain. These features include protein coding genes required for denitrification, diterpenoids catabolism, motility and chemotaxis, protein secretion and production of antimicrobial compounds and insect toxins.ConclusionsThe genome of P. fluorescens F113 is composed of numerous protein-coding genes, not usually found together in previously sequenced genomes, which are potentially decisive during the colonisation of the rhizosphere and/or interaction with other soil organisms. This includes genes encoding proteins involved in the production of a second flagellar apparatus, the use of abietic acid as a growth substrate, the complete denitrification pathway, the possible production of a macrolide antibiotic and the assembly of multiple protein secretion systems.

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Section: Resultsmentioning

confidence: 99%

Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction

et al. 2013

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“…Recently, the genomes of several mosquito derived bacterial strains have been sequences, including Asaia sp., Elizabethkingia anophelis , Enterobacter sp., Pseudomonas sp., Serratia sp., Stenotrophomonas maltophila , and Staphylococcus hominis 55565758596061. These genome data allow one to analyse the microbial genetic repertoire that may be associated with symbiosis.…”

Section: Discussionmentioning

confidence: 99%

Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Chen

Jiang

et al. 2016

Sci Rep

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Sand fly Phlebotomus chinensis is a primary vector of transmission of visceral leishmaniasis in China. The sand flies have adapted to various ecological niches in distinct ecosystems. Characterization of the microbial structure and function will greatly facilitate the understanding of the sand fly ecology, which would provide critical information for developing intervention strategy for sand fly control. In this study we compared the bacterial composition between two populations of Ph. chinensis from Henan and Sichuan, China. The phylotypes were taxonomically assigned to 29 genera of 19 families in 9 classes of 5 phyla. The core bacteria include Pseudomonas and enterobacteria, both are shared in the sand flies in the two regions. Interestingly, the endosymbionts Wolbachia and Rickettsia were detected only in Henan, while the Rickettsiella and Diplorickettsia only in Sichuan. The intracellular bacteria Rickettsia, Rickettsiella and Diplorickettsia were reported for the first time in sand flies. The influence of sex and feeding status on the microbial structure was also detected in the two populations. The findings suggest that the ecological diversity of sand fly in Sichuan and Henan may contribute to shaping the structure of associated microbiota. The structural classification paves the way to function characterization of the sand fly associated microbiome.

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“…This genomic organization appears to be conserved in the genomic sequences of a number of other isolates, mostly originating from plant or soil environments: P. fluorescens strains A506, Q2‐87, SS101, SBW25 (Loper et al ., ), BBc6R8 (Deveau et al ., ), F113 (Redondo‐Nieto et al ., ) and Pf29Arp (Marchi et al ., ), P. poae RE*1‐1‐14 (Müller et al ., ), P. putida W619 (Wu et al ., ), and Pseudomonas spp. Ag1 (Alvarez et al ., ), GM60, GM67 (Brown et al ., ), TKP (Ohtsubo et al ., ) and CF150 (McTee et al ., ).…”

Section: Tailocins: Phage Tail‐like Bacteriocinsmentioning

confidence: 99%

Ribosomally encoded antibacterial proteins and peptides fromPseudomonas

2014

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Members of the Pseudomonas genus produce diverse secondary metabolites affecting other bacteria, fungi or predating nematodes and protozoa but are also equipped with the capacity to secrete different types of ribosomally encoded toxic peptides and proteins, ranging from small microcins to large tailocins. Studies with the human pathogen Pseudomonas aeruginosa have revealed that effector proteins of type VI secretion systems are part of the antibacterial armamentarium deployed by pseudomonads. A novel class of antibacterial proteins with structural similarity to plant lectins was discovered by studying antagonism among plant-associated Pseudomonas strains. A genomic perspective on pseudomonad bacteriocinogeny shows that the modular architecture of S pyocins of P. aeruginosa is retained in a large diversified group of bacteriocins, most of which target DNA or RNA. Similar modularity is present in as yet poorly characterized Rhs (recombination hot spot) proteins and CDI (contact-dependent inhibition) proteins. Well-delimited domains for receptor recognition or cytotoxicity enable the design of chimeric toxins with novel functionalities, which has been applied successfully for S and R pyocins. Little is known regarding how these antibacterials are released and ultimately reach their targets. Other remaining issues concern the identification of environmental triggers activating these systems and assessment of their ecological impact in niches populated by pseudomonads.

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Draft Genome Sequence of Pseudomonas sp. Strain Ag1, Isolated from the Midgut of the Malaria Mosquito Anopheles gambiae

Abstract: A Pseudomonas sp. bacterium was isolated from the midguts of Anopheles gambiae mosquitoes. Here we present the annotated Pseudomonas sp. draft genome sequence as a contribution to the efforts of characterization of the mosquito gut microbiome.

Cited by 17 publications

References 13 publications

Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction

Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction

Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Ribosomally encoded antibacterial proteins and peptides fromPseudomonas

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