Draft Genome Sequence of Pseudomonas plecoglossicida Strain NB2011, the Causative Agent of White Nodules in Large Yellow Croaker (<i>Larimichthys crocea</i>)

Li, Meifang; Chen, Jigang

doi:10.1128/genomea.00586-13

Cited by 31 publications

(32 citation statements)

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“…entomophila and P . aeruginosa , these include, the plant pathogens Pseudomonas syringae , also described as an opportunistic human pathogen [ 57 ], and Pseudomonas amygdali [ 58 ]; Enterobacteriaceae family; the harmful cyanobacteria Microcystis aeruginosa [ 59 ]; the fish pathogen Pseudomonas plecoglossicida [ 60 ]; and the clinical isolates P . putida NBRC 14164T [ 61 ] and Pseudomonas monteilii [ 62 ] ( Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

Specific Gene Loci of Clinical Pseudomonas putida Isolates

et al. 2016

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Pseudomonas putida are ubiquitous inhabitants of soils and clinical isolates of this species have been seldom described. Clinical isolates show significant variability in their ability to cause damage to hosts because some of them are able to modulate the host’s immune response. In the current study, comparisons between the genomes of different clinical and environmental strains of P. putida were done to identify genetic clusters shared by clinical isolates that are not present in environmental isolates. We show that in clinical strains specific genes are mostly present on transposons, and that this set of genes exhibit high identity with genes found in pathogens and opportunistic pathogens. The set of genes prevalent in P. putida clinical isolates, and absent in environmental isolates, are related with survival under oxidative stress conditions, resistance against biocides, amino acid metabolism and toxin/antitoxin (TA) systems. This set of functions have influence in colonization and survival within human tissues, since they avoid host immune response or enhance stress resistance. An in depth bioinformatic analysis was also carried out to identify genetic clusters that are exclusive to each of the clinical isolates and that correlate with phenotypical differences between them, a secretion system type III-like was found in one of these clinical strains, a determinant of pathogenicity in Gram-negative bacteria.

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Section: Resultsmentioning

confidence: 99%

Specific Gene Loci of Clinical Pseudomonas putida Isolates

et al. 2016

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“…Five of them show orthology (> 80% amino acid identity for rhizosphere isolates 36MFCvi1.1, 45MFCol3.1, GM50, GM79, and GM102), but their homology to the other proteins is moderate to low (from c. 50% for strains GM33 and NCIMB 11764 to < 30% for strains CSV86, GB-1, and NB2011). Notably, this strain set encompasses not only several rhizosphere isolates (including GM33) but also strains originating from quite diverse environments: the cyanide-utilizing river mud isolate P. fluorescens NCIMB 11764 (Vilo et al, 2012), the manganeseoxidizing freshwater isolate P. putida GB-1 (Wu et al, 2011b), the naphthalene degrader P. putida CSV86 isolated from soil (Phale et al, 2013), and the fish pathogen P. plecoglossicida NB2011 (Mao et al, 2013).…”

Section: Putative Novel Pyocins With a Tandem Dnase Architecturementioning

confidence: 99%

Ribosomally encoded antibacterial proteins and peptides fromPseudomonas

2014

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Members of the Pseudomonas genus produce diverse secondary metabolites affecting other bacteria, fungi or predating nematodes and protozoa but are also equipped with the capacity to secrete different types of ribosomally encoded toxic peptides and proteins, ranging from small microcins to large tailocins. Studies with the human pathogen Pseudomonas aeruginosa have revealed that effector proteins of type VI secretion systems are part of the antibacterial armamentarium deployed by pseudomonads. A novel class of antibacterial proteins with structural similarity to plant lectins was discovered by studying antagonism among plant-associated Pseudomonas strains. A genomic perspective on pseudomonad bacteriocinogeny shows that the modular architecture of S pyocins of P. aeruginosa is retained in a large diversified group of bacteriocins, most of which target DNA or RNA. Similar modularity is present in as yet poorly characterized Rhs (recombination hot spot) proteins and CDI (contact-dependent inhibition) proteins. Well-delimited domains for receptor recognition or cytotoxicity enable the design of chimeric toxins with novel functionalities, which has been applied successfully for S and R pyocins. Little is known regarding how these antibacterials are released and ultimately reach their targets. Other remaining issues concern the identification of environmental triggers activating these systems and assessment of their ecological impact in niches populated by pseudomonads.

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“…; Mao et al . ,b), retained by the laboratory, was grown on LB agar at 28 °C. ExsA, the predicted transcribing regulator of T3SS in NB2011 (Mao et al .…”

Section: Methodsmentioning

confidence: 99%

“…ExsA, the predicted transcribing regulator of T3SS in NB2011 (Mao et al . ), sharing amino acid sequence identity with ExsA of P. aeruginosa of 64%, is essential for expression of the T3SS proteins (Schulert et al . ), and the exsA ‐deleted strain, NB2011∆ExsA mutant, was generated by allelic exchange (Donnenberg & Kaper ; Philippe et al .…”

Section: Methodsmentioning

confidence: 99%

“…). Genomic annotation of a pathogenic strain of P. plecoglossicida (NB2011) disclosed a typical type III secretion system (T3SS) located on a genomic island, and the gene organization and sequences of the structural proteins were similar to the T3SS of Pseudomonas aeruginosa (Mao, Li & Chen ). A predicted effector protein has been identified and named as PP_exoU, which showed 45% amino acid sequence identity to exoU, the major phospholipase cytotoxin of P. aeruginosa (Sato et al .…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of a phospholipase A1 activity type three secreted protein, PP_ExoU from Pseudomonas plecoglossicida NB2011, the causative agent of visceral granulomas disease in large yellow croaker (Larimichthys crocea)

Zhang

Guo

et al. 2016

Journal of Fish Diseases

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Pseudomonas plecoglossicida NB2011, the causative agent of visceral granulomas disease in farmed Larimichthys crocea in China, encodes a predicted type three effector PP_ExoU, a homolog of the cytotoxin ExoU of Pseudomonas aeruginosa. In this study, secretion of PP_ExoU was tested in various broth, the protein was expressed with the pET30a prokaryotic system, the phospholipase A (PLA) activity of the recombinant protein was determined with fluorogenic phospholipid substrates, fusion expression with green fluorescent protein in transfected HeLa cells was investigated, and the lactate dehydrogenase (LDH) level was measured. The results showed the protein was type three secreted in several media; the recombinant protein displayed significant PLA1 activity with ubiquitin. Fluorescence was observed on the cell membrane and scattered in the cytoplasm of HeLa cells expressing catalytic wild-type PP_ExoU, blebbing and stretching developed in the cell membranes indicating of membrane damage. Fluorescence scattered in the cytoplasm of cells expressing the catalytic inactive protein. A significant LDH level was detected in HeLa cells expressing wild-type PP_exoU, but not in the Ser/Asp-mutated protein, suggestion mutation of predicted catalytic residues abolished the PLA activity. This is the first report on the function of a secreted type three protein from P. plecoglossicida.

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Draft Genome Sequence of Pseudomonas plecoglossicida Strain NB2011, the Causative Agent of White Nodules in Large Yellow Croaker (Larimichthys crocea)

Cited by 31 publications

References 10 publications

Specific Gene Loci of Clinical Pseudomonas putida Isolates

Specific Gene Loci of Clinical Pseudomonas putida Isolates

Ribosomally encoded antibacterial proteins and peptides fromPseudomonas

Identification and characterization of a phospholipase A1 activity type three secreted protein, PP_ExoU from Pseudomonas plecoglossicida NB2011, the causative agent of visceral granulomas disease in large yellow croaker (Larimichthys crocea)

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