Draft Genome Sequence of Sporolactobacillus inulinus NBRC 111894, Isolated from Kôso, a Japanese Sugar-Vegetable Fermented Beverage

Chiou, Tai-Ying; Suda, Wataru; Oshima, Koichiro; Hattori, Masahira; Matsuzaki, Chiaki; Yamamoto, Kenji; Takahashi, Tomoya

doi:10.1128/mra.00751-19

Cited by 3 publications

(1 citation statement)

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“…Due to its use of fresh materials and a spontaneous fermentation process, it contains a complex microbial community [1]. Some lactic acid bacteria (LAB), such as Companilactobacillus farciminis , Apilactobacillus kosoi and Sporolactobacillus inulinus , have been isolated from kôso and their genomic information reported [2–5]. A.…”

Section: Full-textmentioning

confidence: 99%

Lentilactobacillus kosonis sp. nov., isolated from kôso, a Japanese sugar-vegetable fermented beverage

Chiou

Suda

Oshima

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel lactic acid-producing, Gram-stain-positive, catalase-negative and rod-shaped strain, designated as strain C06_No.73T, was isolated from a traditional Japanese fermented beverage called kôso. According to the results of phylogenetic analysis based on 16S rRNA gene sequences, strain C06_No.73T belongs to the genus Lentilactobacillus . The closest type strain was Lentilactobacillus curieae CCTCC M 2011381T, with a sequence identity of 98.1 %. The identity values with other strains were all below 97 %. The isolate propagated under the conditions of 18–39 °C (optimum, 27 °C for 48 h incubation) and pH 4.0–7.0 (optimum, pH 6.5). The G+C content of its genomic DNA was determined to be 37.9 mol%. The main fatty acids were C16 : 0, C18 : 1 ω7c, C18 : 1 ω9c and C19 : 0 cyclopropane 11,12. The major polar lipid was identified as phosphatidylglycerol. No isoprenoid quinone was detected. The predominant cell-wall amino acids were lysine, alanine, glutamic acid and aspartic acid. Neither meso-diaminopimelic acid nor ornithine were detected. On the basis of this polyphasic taxonomic study, the isolate is concluded to represent a novel species, for which the name Lentilactobacillus kosonis sp. nov. is proposed. The type strain is C06_No.73T (=NBRC 111893T=BCRC 81282T).

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