Draft Genome Sequence of the Toxic Freshwater Microcystis aeruginosa Strain PMC 728.11 (Cyanobacteria, Chroococcales)

Halary, Sébastien; Duval, Clément; Gallet, Alison; Duperron, Sébastien; Piquet, Bérénice; Demay, Justine; Bernard, Cécile; Marie, Benjamin

doi:10.1128/mra.01096-20

Cited by 6 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cyanobacterial strain PMC 728.11 contains various secondary metabolites that may be responsible for variations in fish gut microbiota. Among those already identified and specifically those potentially produced by the strain (24,69), cyanopeptides, such as aerucyclamides and bacteriocins, are thought to exhibit potent antimicrobial or cytotoxic bioactivities (70,71), and could directly impact the microbiota during Microcystis cell digestion into the intestine lumen.…”

Section: Discussionmentioning

confidence: 99%

Disruption of fish gut microbiota composition and holobiont’s metabolome by cyanobacterial blooms

Gallet

Halary

Duval

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Cyanobacterial blooms are one of the most common stress encountered by metazoans living in freshwater lentic systems such as lakes and ponds. Blooms reportedly impair fish health, notably through oxygen depletion and production of bioactive compounds including cyanotoxins. However, in the times of the microbiome revolution, it is surprising that so little is still known regarding the influence of blooms on fish microbiota. In this study, an experimental approach is used to demonstrate that blooms affect fish microbiome composition and functions, as well as the metabolome of holobionts. To this end, the model teleost Oryzias latipes is exposed to simulated Microcystis aeruginosa blooms of various intensities in a microcosm setting, and the response of bacterial gut communities is evaluated in terms of composition, metagenome-encoded functions and metabolome profiling. Results: The gut bacterial community of O. latipes exhibits marked responses to the presence of M. aeruginosa blooms in a dose-dependent manner. Notably, abundant gut-associated Firmicutes almost disappear, while potential opportunists increase. The holobiont gut metabolome displays major changes, while functions encoded in the metagenome of bacterial partners are more marginally affected. Bacterial communities tend to return to original composition after the end of the bloom suggesting post-bloom resilience, and remain sensitive in case of a second bloom, reflecting a highly reactive gut community. Conclusion: In the context of increasingly frequent and intense blooms worldwide, results point to the relevance of accounting for short- and long-term microbiome-related effects in fish ecology, with potential outcomes relevant to conservation biology as well as aquaculture.

show abstract

Section: Discussionmentioning

confidence: 99%

Disruption of fish gut microbiota composition and holobiont’s metabolome by cyanobacterial blooms

Gallet

Halary

Duval

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…At day 0, nine fish in total were sampled randomly across the six aquaria and 25 mL of water from each aquarium were pooled as controls. Fish were then exposed for 4 days to water, or water containing a strong but environmentally relevant density of the live non-axenic mono-clonal M. aeruginosa strain PMC 728.11 15 of the Paris Museum Collection 16 to simulate a bloom (100 µ g.L -1 Chl a ). The strain was cultured accordingly to anterior publication 11,14 (details in the supplementary information).…”

Section: Methodsmentioning

confidence: 99%

Gut microbiota and holobiont metabolome composition of the Medaka fish (Oryzias latipes) are affected by a short exposure to the cyanobacterium Microcystis aeruginosa

Foucault

Gallet

Duval

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Blooms of toxic cyanobacteria are a common stress encountered by aquatic fauna. Evidence indicates that long-lasting blooms affect fauna-associated microbiota. Because of their multiple roles, host-associated microbes are nowadays considered relevant to ecotoxicology, yet the respective timing of microbiota versus functional changes in holobionts response needs to be clarified. The response of gut microbiota and holobionts metabolome to exposure to a dense culture of Microcystis aeruginosa was investigated as a microcosm-simulated bloom in the model fish species Oryzias latipes (medaka). Both gut microbiota and gut metabolome displayed significant composition changes after only 2 days of exposure. A dominant symbiont, member of the Firmicutes, plummeted whereas various genera of Proteobacteria and Actinobacteriota increased in relative abundance. Changes in microbiota composition occurred earlier and faster compared to metabolome composition, suggesting that the microbiota drives the holobionts response. Liver and muscle metabolome were much less affected than guts, supporting that gut and associated microbiota are in the front row upon exposure. This study highlights that even short cyanobacterial blooms, that are increasingly frequent, trigger changes in microbiota composition and holobiont metabolome. It emphasizes the relevance of multi-omics approaches to explore organisms response to an ecotoxicological stress.

show abstract

“…The 24 strains from Champs-sur-Marne used for cultures were among the 25 ones selected for genome sequencing. Strains were isolated as previously described (Halary et al, 2020). All strains were grown at 25 C for 1 month with a 16:8 photoperiod (day/night) and at a light intensity of 10-15 μmol photon m À2 s À1 .…”

Section: Genome Sequencing and Analysesmentioning

confidence: 99%

Widespread formation of intracellular calcium carbonates by the bloom‐forming cyanobacterium Microcystis

Gaëtan

Halary

Millet

et al. 2022

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

The formation of intracellular amorphous calcium carbonates (iACC) has been recently observed in a few cultured strains of Microcystis, a potentially toxic bloom‐forming cyanobacterium found worldwide in freshwater ecosystems. If iACC‐forming Microcystis are abundant within blooms, they may represent a significant amount of particulate Ca. Here, we investigate the significance of iACC biomineralization by Microcystis. First, the presence of iACC‐forming Microcystis cells has been detected in several eutrophic lakes, indicating that this phenomenon occurs under environmental conditions. Second, some genotypic (presence/absence of ccyA, a marker gene of iACC biomineralization) and phenotypic (presence/absence of iACC) diversity have been detected within a collection of strains isolated from one single lake. This illustrates that this trait is frequent but also variable within Microcystis even at a single locality. Finally, one‐third of publicly available genomes of Microcystis were shown to contain the ccyA gene, revealing a wide geographic and phylogenetic distribution within the genus. Overall, the present work shows that the formation of iACC by Microcystis is common under environmental conditions. While its biological function remains undetermined, this process should be further considered regarding the biology of Microcystis and implications on the Ca geochemical cycle in freshwater environments.

show abstract

Draft Genome Sequence of the Toxic Freshwater Microcystis aeruginosa Strain PMC 728.11 (Cyanobacteria, Chroococcales)

Cited by 6 publications

References 20 publications

Disruption of fish gut microbiota composition and holobiont’s metabolome by cyanobacterial blooms

Disruption of fish gut microbiota composition and holobiont’s metabolome by cyanobacterial blooms

Gut microbiota and holobiont metabolome composition of the Medaka fish (Oryzias latipes) are affected by a short exposure to the cyanobacterium Microcystis aeruginosa

Widespread formation of intracellular calcium carbonates by the bloom‐forming cyanobacterium Microcystis

Contact Info

Product

Resources

About