Draft Genome Sequence of the Novel Thermoacidophilic Archaeon
            <i>Acidianus copahuensis</i>
            Strain ALE1, Isolated from the Copahue Volcanic Area in Neuquén, Argentina

Urbieta, María Sofía; Rascovan, Nicolás; Castro, Camila; Revale, Santiago; Giaveno, María Alejandra; Vázquez, Martı́n; Donati, Edgardo R.

doi:10.1128/genomea.00259-14

Cited by 17 publications

(16 citation statements)

References 6 publications

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“…The ability of strain DS80 to oxidize As(III) is consistent with the presence of aioAB genes encoding the arsenite oxidase in the DS80 genome (Amenabar et al, submitted). Homologs of these genes have been previously reported in the genomes of other members of the Acidianus genus, including A. hospitalis (58) and A. copahuensis (47,61). This study reports a thermoacidophile capable of oxidizing ␣-As 4 S 4 , either directly or indirectly, to support growth.…”

Section: Discussionsupporting

confidence: 51%

Mechanisms of Mineral Substrate Acquisition in a Thermoacidophile

Amenábar

Boyd

2018

Appl Environ Microbiol

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The thermoacidophile is widely distributed in Yellowstone National Park hot springs that span large gradients in pH (1.60 to 4.84), temperature (42 to 90°C), and mineralogical composition. To characterize the potential role of flexibility in mineral-dependent energy metabolism in contributing to the widespread ecological distribution of this organism, we characterized the spectrum of minerals capable of supporting metabolism and the mechanisms that it uses to access these minerals. The energy metabolism of strain DS80 was supported by elemental sulfur (S), a variety of iron (hydr)oxides, and arsenic sulfide. Strain DS80 reduced, oxidized, and disproportionated S Cells growing via S reduction and disproportionation did not require direct access to the mineral to reduce it, whereas cells growing via S oxidation did require direct access, observations that are attributable to the role of HS produced by S reduction/disproportionation in solubilizing and increasing the bioavailability of S Cells growing via iron (hydr)oxide reduction did not require access to the mineral, suggesting that the cells reduce Fe(III) that is being leached by the acidic growth medium. Cells growing via oxidation of arsenic sulfide with Fe(III) did not require access to the mineral to grow. The stoichiometry of reactants to products indicates that cells oxidize soluble As(III) released from oxidation of arsenic sulfide by aqueous Fe(III). Taken together, these observations underscore the importance of feedbacks between abiotic and biotic reactions in influencing the bioavailability of mineral substrates and defining ecological niches capable of supporting microbial metabolism. Mineral sources of electron donor and acceptor that support microbial metabolism are abundant in the natural environment. However, the spectrum of minerals capable of supporting a given microbial strain and the mechanisms that are used to access these minerals in support of microbial energy metabolism are often unknown, in particular among thermoacidophiles. Here, we show that the thermoacidophile strain DS80 is adapted to use a variety of iron (hydro)oxide minerals, elemental sulfur, and arsenicsulfide to support growth. Cells rely on a complex interplay of abiologically and biologically catalyzed reactions that increase the solubility or bioavailability of minerals, thereby enabling their use in microbial metabolism.

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Section: Discussionsupporting

confidence: 51%

Mechanisms of Mineral Substrate Acquisition in a Thermoacidophile

Amenábar

Boyd

2018

Appl Environ Microbiol

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“…Furthermore, being the shortest members of the “7 kDa DNA-binding” family, these three proteins are particularly attractive considering the importance of a minimal size for biomedical applications. The proteins from Candidatus Acidianus copahuensis 27 and from Candidatus Aramenus sulfurataquae 26 were not studied as their sequences were released after the beginning of this study.…”

Section: Resultsmentioning

confidence: 99%

The archaeal “7 kDa DNA-binding” proteins: extended characterization of an old gifted family

Kalichuk

Béhar

Renodon-Cornière

et al. 2016

Sci Rep

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The “7 kDa DNA-binding” family, also known as the Sul7d family, is composed of chromatin proteins from the Sulfolobales archaeal order. Among them, Sac7d and Sso7d have been the focus of several studies with some characterization of their properties. Here, we studied eleven other proteins alongside Sac7d and Sso7d under the same conditions. The dissociation constants of the purified proteins for binding to double-stranded DNA (dsDNA) were determined in phosphate-buffered saline at 25 °C and were in the range from 11 μM to 22 μM with a preference for G/C rich sequences. In accordance with the extremophilic origin of their hosts, the proteins were found highly stable from pH 0 to pH 12 and at temperatures from 85.5 °C to 100 °C. Thus, these results validate eight putative “7 kDa DNA-binding” family proteins and show that they behave similarly regarding both their function and their stability among various genera and species. As Sac7d and Sso7d have found numerous uses as molecular biology reagents and artificial affinity proteins, this study also sheds light on even more attractive proteins that will facilitate engineering of novel highly robust reagents.

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“…To date, only the genomes of A. copahuensis and A. hospitalis have been sequenced [46,48]. The common characteristic displayed by all Acidianus species is their ability to oxidize or reduce elemental sulfur, depending on oxygen availability.…”

Section: The Genus Acidianusmentioning

confidence: 99%

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

et al. 2015

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Extreme thermoacidophiles (Topt > 65 °C, pHopt < 3.5) inhabit unique environments fraught with challenges, including extremely high temperatures, low pH, as well as high levels of soluble metal species. In fact, certain members of this group thrive by metabolizing heavy metals, creating a dynamic equilibrium between biooxidation to meet bioenergetic needs and mechanisms for tolerating and resisting the toxic effects of solubilized metals. Extremely thermoacidophilic archaea dominate bioleaching operations at elevated temperatures and have been considered for processing certain mineral types (e.g., chalcopyrite), some of which are recalcitrant to their mesophilic counterparts. A key issue to consider, in addition to temperature and pH, is the extent to which solid phase heavy metals are solubilized and the concomitant impact of these mobilized metals on the microorganism's growth physiology. Here, extreme thermoacidophiles are examined from the perspectives of biodiversity, heavy metal biooxidation, metal resistance mechanisms, microbe-solid interactions, and application of these archaea in biomining operations.

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Draft Genome Sequence of the Novel Thermoacidophilic Archaeon Acidianus copahuensis Strain ALE1, Isolated from the Copahue Volcanic Area in Neuquén, Argentina

Cited by 17 publications

References 6 publications

Mechanisms of Mineral Substrate Acquisition in a Thermoacidophile

Mechanisms of Mineral Substrate Acquisition in a Thermoacidophile

The archaeal “7 kDa DNA-binding” proteins: extended characterization of an old gifted family

The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles

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