Draft Genome Sequence of the Antimicrobial-Producing Strain Paenibacillus elgii AC13

Ortega, Daniel Barros; Costa, Rosiane Andrade da; Pires, Állan S.; Araújo, Thiago F.; Araújo, Janaina Fernandez; Kurokawa, Adriane Silva; Magalhães, Beatriz Simas; Reis, Alessandra Maria Moreira; Franco, Octávio L.; Krüger, Ricardo Henrique; Pappas, George J.; Barreto, Cristine Chaves

doi:10.1128/genomea.00573-18

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…quinquefasciatus (Table ). The Paenibacillus elgii AC13 strain was isolated from Cerrado soil and is known for producing lipopeptides belonging to the pelgipeptin family, which presents antimicrobial activity. − …”

Section: Methodsmentioning

confidence: 99%

Pelgipeptins, a Nonribosomal Lipopeptide Family, Show Larvicidal Activity against Vectors Transmitting Viruses

Costa

Dutra

Andrade

et al. 2023

J. Agric. Food Chem.

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Aedes aegypti and Culex quinquefasciatus are vectors of numerous diseases of worldwide public importance, such as arboviruses and filariasis. The main strategy for controlling these vectors is the use of chemicals, which can induce the appearance of resistant insects. The use of Bacillus thuringiensis (Bt) and Lysinibacillus sphaericus (Ls) with larvicidal activity against arboviraltransmitting insects has been successful in many studies. In contrast, the use and knowledge of peptides with insecticidal activity are so far scarce. In this work, 25 peptides and 5 strains of each bacterial species were prospected individually or together regarding their insecticidal activity. Initially, in vitro assays of cellular cytotoxicity of the peptides against SF21 cells of Spodoptera frugiperda were performed. The peptides Polybia-MPII and pelgipeptin caused 69 and 60% of cell mortality, respectively, at the concentration of 10 μM. Thus, they were evaluated in vivo against second-stage larvae of the two Culicidae. However, in the in vivo bioassays, only pelgipeptin showed larvicidal mortality against both larvae (LC 50 6.40 μM against A. aegypti, and LC 50 1.22 μM against C. quinquefasciatus). The toxin-producing bacterial strain that showed the lowest LC 50 against A. aegypti was Bt S8 (LC 50 = 0.71 ng/ mL) and against C. quinquefasciatus, it was Ls S260 (LC 50 = 2.32 ng/mL). So, the synergistic activity between the association of the bacterial toxins and pelgipeptin was evaluated. A synergic effect of pelgipeptin was observed with Ls strain S260 against C. quinquefasciatus. Our results demonstrate the possibility of synergistic or individual use of both biologically active larvicides against C. quinquefasciatus and A. aegypti.

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Section: Methodsmentioning

confidence: 99%

Pelgipeptins, a Nonribosomal Lipopeptide Family, Show Larvicidal Activity against Vectors Transmitting Viruses

Costa

Dutra

Andrade

et al. 2023

J. Agric. Food Chem.

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“…Paenibacillus elgii AC13 was isolated from Brazilian Cerrado soil [23] , and it produced four pelgipeptin isoforms as found in P. elgii B69 [20] , [21] ( Table 1 ). The purified spores of P. elgii AC13 were inoculated in 250 mL flasks containing 100 mL of MMP medium (pH 7.0) (Patent BR102017018881 7) at a final concentration of 10 3 spores/mL and incubated at 37 °C, 200 rpm, for 72 hours.…”

Section: Methodsmentioning

confidence: 99%

<i>In vitro</i> antifungal activity of pelgipeptins against human pathogenic fungi and <i>Candida albicans</i> biofilms

Fulgêncio¹,

Costa²,

Guilhelmelli³

et al. 2021

AIMS Microbiology

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Systemic mycoses have become a major cause of morbidity and mortality, particularly among immunocompromised hosts and long-term hospitalized patients. Conventional antifungal agents are limited because of not only their costs and toxicity but also the rise of resistant strains. Lipopeptides from Paenibacillus species exhibit antimicrobial activity against a wide range of human and plant bacterial pathogens. However, the antifungal potential of these compounds against important human pathogens has not yet been fully evaluated, except for Candida albicans . Paenibacillus elgii produces a family of lipopeptides named pelgipeptins, which are synthesized by a non-ribosomal pathway, such as polymyxin. The present study aimed to evaluate the activity of pelgipeptins produced by P. elgii AC13 against Cryptococcus neoformans , Paracoccidioides brasiliensis , and Candida spp. Pelgipeptins were purified from P. elgii AC13 cultures and characterized by high-performance liquid chromatography (HPLC) and mass spectrometry (MALDI-TOF MS). The in vitro antifugal activity of pelgipeptins was evaluated against C. neoformans H99, P. brasiliensis PB18, C. albicans SC 5314, Candida glabrata ATCC 90030, and C. albicans biofilms. Furthermore, the minimal inhibitory concentration (MIC) was determined according to the CLSI microdilution method. Fluconazole and amphotericin B were also used as a positive control. Pelgipeptins A to D inhibited the formation and development of C. albicans biofilms and presented activity against all tested microorganisms. The minimum inhibitory concentration values ranged from 4 to 64 µg/mL, which are in the same range as fluconazole MICs. These results highlight the potential of pelgipeptins not only as antimicrobials against pathogenic fungi that cause systemic mycoses but also as coating agents to prevent biofilm formation on medical devices.

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A novel family of non-secreted tridecaptin lipopeptide produced by Paenibacillus elgii

et al. 2022

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Draft Genome Sequence of the Antimicrobial-Producing Strain Paenibacillus elgii AC13

Abstract: A Paenibacillus elgii strain isolated from soil samples from Cerrado, Brazil, showed antimicrobial activity. Its genome sequence was acquired (GS20 FLX Titanium 454 platform) and comprises 108 contigs (N50, 198,427 bp) and 6,810 predicted sequences.

Cited by 7 publications

References 15 publications

Pelgipeptins, a Nonribosomal Lipopeptide Family, Show Larvicidal Activity against Vectors Transmitting Viruses

Pelgipeptins, a Nonribosomal Lipopeptide Family, Show Larvicidal Activity against Vectors Transmitting Viruses

<i>In vitro</i> antifungal activity of pelgipeptins against human pathogenic fungi and <i>Candida albicans</i> biofilms

A novel family of non-secreted tridecaptin lipopeptide produced by Paenibacillus elgii

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