Draft Genome Sequence of Thiostrepton-Producing Streptomyces azureus ATCC 14921

Abstract

Streptomyces azureus ATCC 14921 belongs to the Streptomyces cyaneus cluster and is known to be a thiostrepton producer. Here, we report a draft genome sequence for this strain, consisting of 350 contigs containing a total of 8,790,525 bp, 8,164 predicted coding sequences, and a G+C content of 70.9%.

Whole genome sequencing of Streptomyces actuosus ISP-5337, Streptomyces sioyaensis B-5408, and Actinospica acidiphila B-2296 reveals secondary metabolomes with antibiotic potential

“…Due to the variability in sequencing technologies, only 94 species were reported with full-length 16S rRNA sequences in the NCBI RefSeq database. Our phylogenetic analysis shows the placement of our three sequenced species in three separate clades, as previously observed [ [56] , [57] , [58] ].…”

“…A third member of the unique thiopeptides harboring both RiPP and NRPS character in their BGCs is thiostrepton. S. azureus and S. cyaneus CGMCC 4.1671 are known producers of thiostrepton [ 58 , 76 , 77 ]; however, whole genome sequencing of S. cyaneus B-2296 T revealed that the strain we received was misannotated as S. cyaneus and should be reclassified as A. acidiphila B-2296 (Fig. S5).…”

Complete Genome Sequence of Thiostrepton-Producing Streptomyces laurentii ATCC 31255

“…In addition, a gene cluster for thiostrepton biosynthesis was annotated from genes SLA3859 ( tsrS ) to SLA3877 ( tsrA ) ( 11 ). This cluster shows strong similarity to the thiostrepton biosynthesis cluster of S. azureus ( 12 ). The tsr gene is located between the EF-Tu gene and the N -acetylmuramoyl- l -alanine amidase family protein-coding gene.…”