Draft Genome Sequence of Xanthomonas arboricola pv. juglandis J303, Isolated from Infected Walnut Trees in Southern Chile

Abstract

Here, we report the draft genome sequence of Xanthomonas arboricola pv. juglandis J303, isolated from infected walnut trees in southern Chile. The size of the genome is 5,066,424 bp with a G+C content of 65.4%. X. arboricola pv. juglandis J303 has several genes related to virulence, antibiotic resistance, and copper resistance.

Characterization of Xanthomonas arboricola pv. juglandis Bacteriophages against Bacterial Walnut Blight and Field Evaluation

“…For the bacteriophage isolation, the strain denominated X. arboricola pv. juglandis J303 was employed [ 11 ]. This isolate is rod-shaped, 0.5 × 2.5 µm in size, surrounded by a dense polymeric matrix, and able to develop robust damage in the in vitro walnut fruit pathogenicity assay ( Figure S1 ).…”

“…The pathogenicity of these bacterial isolates was confirmed in immature walnut fruits [ 21 ]. For comparative purposes, the isolate X. juglandis J303 was included in all assays [ 11 ]. Purified colonies identified as X. juglandis were kept at −80 °C in a mixture of glycerol (20% v / v ) and YPG broth.…”

“…For this, previously, f 20-Xaj and f 30-Xaj genomes were sequenced through Illumina MiSeq and assembled using CLC Genomics Workbench 8.5.1 (Qiagen, Hilden, Germany). Both assembled genome sequences were annotated by the National Center for Biotechnology Information (NCBI, Bethesda, MD, USA) Prokaryotic Genomes Annotation Pipeline (PGAP) and deposited with GenBank accession numbers of KU595432 and KU595433, respectively [ 11 ]. The Neighbor-Joining method was used to infer the evolutionary history [ 37 ].…”

“…The efficiency of copper-base agrochemicals has decreased, due to the emergence of X. juglandis- resistant strains [ 6 , 7 , 8 ]. Recently, genomic studies showed that the X. juglandis genome had several genes related to copper resistance mechanisms [ 9 , 10 , 11 , 12 ]. Additionally, the utilization of heavy metals in a high dose, such as copper, during the production process has serious agronomic and sanitary implications, which results in rejection by consumers [ 13 ].…”

Comparative Genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis Strains Isolated from a Single Walnut Host Tree

“…The genome size of CPBF 427 (5.23 Mbp) is roughly equal to the genome size reported for other sequenced X. arboricola pv. juglandis genomes, namely Xaj 417 [ 62 ], NCPPB 1447 [ 9 ], J303 [ 63 ], DW3F3, [ 64 ], and CFBP 2528 T and CFBP 7179 [ 24 ] or CFSAN033077 and CFSAN033080 [ 65 ]. The genomes of X. euroxanthea strains CPBF 367, CPBF 424 T , and CPBF 426 were smaller (ranging from ≈ 4.90 to 4.97 Mbp), presenting values closer to the nonpathogenic or avirulent strains of X. arboricola , i.e., with uncertain pathogenicity or belonging to non- juglandis , non- pruni , and non- corylina pathovars, with genome sizes inferior to 5 Mb [ 66 , 67 , 68 ].…”