Draft Genome Sequences for 10 Isolates of the Swine Pathogen Haemophilus parasuis

Kuehn, Joanna S.; Register, Karen B.; Phillips, Gregory J.

doi:10.1128/genomea.00739-13

Cited by 15 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, this protein is involved in the acquisition of iron from the surrounding environment, which is recognized as one significant factor in the successful establishment of the pathogen in the host. Until recently, the complete genomes for 10 isolates of H. parasuis were sequenced [ 37 ]. MASCOT was used to search the data against the NCBInr protein database, and spots 6702 and 6703 matched the tonB-dependent siderophore receptor family protein in H. parasuis (Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

et al. 2014

View full text Add to dashboard Cite

BackgroundHaemophilus parasuis is the causative agent of Glässer’s disease characterized by polyserositis, arthritis, and meningitis in pig, leading to serious economic loss. Despite many years of study, virulence factors and the mechanisms of the entire infection process remain largely unclear. So two-dimensional gel electrophoresis and mass spectrometry were used to search for distinctions at the membrane protein expression level between two H. parasuis isolates aimed at uncovering some proteins potentially involved in habitat adaption and pathogenesis.ResultsA comparative proteomic approach combining two-dimensional gel electrophoresis with mass spectrometry and tandem mass spectrometry was employed to explore the differences among membrane proteomes of a virulent Haemophilus parasuis strain isolated from the lung of a diseased pig and an avirulent strain isolated from the nasal swab of a healthy pig. Differentially expressed protein spots identified by mass spectrometry were annotated and analyzed by bioinformatic interpretation. The mRNA level was determined by quantitative real-time PCR. Proteins representing diverse functional activities were identified. Among them, the tonB-dependent siderophore receptor was a new discovery highlighted for its activity in iron uptake. In addition, periplasmic serine protease and putrescine/spermidine ABC transporter substrate-binding protein were given focus because of their virulence potential. This study revealed that the differentially expressed proteins were important in either the habitat adaption or pathogenesis of H. parasuis.ConclusionsThe outcome demonstrated the presence of some proteins which raise the speculation for their importance in helping in habitat adaption or pathogenesis within the host.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…During the preparation of this paper, ten additional H. parasuis genome drafts, including one of the Nagasaki strain genome, have been deposited in GenBank (Kuehn et al , 2013). In the study by Kuehn et al (2013), sequencing of the Nagasaki strain resulted in assembly of 78 contigs, making the draft genome of the present study more suitable for genome comparison. Nonetheless, it is expected that the analysis of a higher number of genomes will shed more light on the genetics of this bacterium.…”

Section: Discussionmentioning

confidence: 99%

“…In the study by Kuehn et al (2013), sequencing of the Nagasaki strain resulted in assembly of 78 contigs, making the draft genome of the present study more suitable for genome comparison. Nonetheless, it is expected that the analysis of a higher number of genomes will shed more light on the genetics of this bacterium.…”

Section: Whereasmentioning

confidence: 95%

Genome comparison of three serovar 5 pathogenic strains of Haemophilus parasuis: insights into an evolving swine pathogen

et al. 2014

View full text Add to dashboard Cite

Haemophilus parasuis is the causative agent of Glä sser's disease, a systemic disorder characterized by polyarthritis, polyserositis and meningitis in pigs. Although it is well known that H. parasuis serovar 5 is the most prevalent serovar associated with the disease, the genetic differences among strains are only now being discovered. Genomes from two serovar 5 strains, SH0165 and 29755, are already available. Here, we present the draft genome of a third H. parasuis serovar 5 strain, the formal serovar 5 reference strain Nagasaki. An in silico genome subtractive analysis with full-length predicted genes of the three H. parasuis serovar 5 strains detected 95, 127 and 95 strain-specific genes (SSGs) for Nagasaki, SH0165 and 29755, respectively. We found that the genomic diversity within these three strains was high, in part because of a high number of mobile elements. Furthermore, a detailed analysis of large sequence polymorphisms (LSPs), encompassing regions ranging from 2 to 16 kb, revealed LSPs in virulence-related elements, such as a Toll-IL receptor, the AcrA multidrug efflux protein, an ATPbinding cassette (ABC) transporter, lipopolysaccharide-synthetizing enzymes and a tripartite ATP-independent periplasmic (TRAP) transporter. The whole-genome codon adaptation index (CAI) was also calculated and revealed values similar to other well-known bacterial pathogens. In addition, whole-genome SNP analysis indicated that nucleotide changes tended to be increased in membrane-related genes. This analysis provides further evidence that the genome of H. parasuis has been subjected to multiple lateral gene transfers (LGTs) and to fine-tuning of virulence factors, and has the potential for accelerated genome evolution.

show abstract

“…Libraries were sequenced at the Iowa State University DNA Facility on an Illumina GAIIx for either 75 cycles (strains SW114, 12939, 84-15995, H465, and D74) or 100 cycles (strains Nagasaki, MN-H, 29755, 174 and SW140). GenBank accession numbers for the genomes are given in Table 2 and Kuehn et al [36] .…”

Section: Methodsmentioning

confidence: 99%

Virulence and Draft Genome Sequence Overview of Multiple Strains of the Swine Pathogen Haemophilus parasuis

Brockmeier

Kuehn

et al. 2014

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Haemophilus parasuis is the cause of Glässer's disease in swine, which is characterized by systemic infection resulting in polyserositis, meningitis, and arthritis. Investigation of this animal disease is complicated by the enormous differences in the severity of disease caused by H. parasuis strains, ranging from lethal systemic disease to subclinical carriage. To identify differences in genotype that could account for virulence phenotypes, we established the virulence of, and performed whole genome sequence analysis on, 11 H. parasuis strains. Virulence was assessed by evaluating morbidity and mortality following intranasal challenge of Caesarean-derived, colostrum-deprived (CDCD) pigs. Genomic DNA from strains Nagasaki (serotype 5), 12939 (serotype 1), SW140 (serotype 2), 29755 (serotype 5), MN-H (serotype 13), 84-15995 (serotype 15), SW114 (serotype 3), H465 (serotype 11), D74 (serotype 9), and 174 (serotype 7) was used to generate Illumina paired-end libraries for genomic sequencing and de novo assembly. H. parasuis strains Nagasaki, 12939, SH0165 (serotype 5), SW140, 29755, and MN-H exhibited a high level of virulence. Despite minor differences in expression of disease among these groups, all pigs challenged with these strains developed clinical signs consistent with Glässer's disease between 1–7 days post-challenge. H. parasuis strains 84-15995 and SW114 were moderately virulent, in that approximately half of the pigs infected with each developed Glässer's disease. H. parasuis strains H465, D74, and 174 were minimally virulent or avirulent in the CDCD pig model. Comparative genomic analysis among strains identified several noteworthy differences in coding regions. These coding regions include predicted outer membrane, metabolism, and pilin or adhesin related genes, some of which likely contributed to the differences in virulence and systemic disease observed following challenge. These data will be useful for identifying H. parasuis virulence factors and vaccine targets.

show abstract

Draft Genome Sequences for 10 Isolates of the Swine Pathogen Haemophilus parasuis

Cited by 15 publications

References 8 publications

Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

Genome comparison of three serovar 5 pathogenic strains of Haemophilus parasuis: insights into an evolving swine pathogen

Virulence and Draft Genome Sequence Overview of Multiple Strains of the Swine Pathogen Haemophilus parasuis

Contact Info

Product

Resources

About