2021
DOI: 10.1128/mra.00854-21
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Draft Genome Sequences of Multidrug-Resistant Shigella Strains Isolated from Diarrheal Patients in Bangladesh

Abstract: The emergence of multidrug-resistant (MDR) Shigella strains has impaired the efficacy of first-line antimicrobials and exacerbated diarrhea-associated morbidity and mortality worldwide. We report the draft genome sequences of 11 MDR Shigella strains isolated from the stool specimens of diarrheal patients in Bangladesh.

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Cited by 5 publications
(6 citation statements)
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“…We determined the bacterial susceptibility to antimicrobial agents by the disc diffusion method according to the guidelines of CLSI 46 using Muller-Hinton agar and commercially available antimicrobial discs (Oxoid, Basingstoke, United Kingdom) 57 . We used E. coli (ATCC 25922) strain as negative control in AST.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…We determined the bacterial susceptibility to antimicrobial agents by the disc diffusion method according to the guidelines of CLSI 46 using Muller-Hinton agar and commercially available antimicrobial discs (Oxoid, Basingstoke, United Kingdom) 57 . We used E. coli (ATCC 25922) strain as negative control in AST.…”
Section: Methodsmentioning
confidence: 99%
“…Three MDR- Shigella having similar pattern of AMR and conferring middle-range plasmid were subjected to whole genome sequencing using Illumina technologies. Sequence data was processed and assembled using the bio-informatics techniques described previously 57 . Resistome profiling was performed using the AMRFinderPlus v3.10.5 65 and PlasmidFinder v2.1 ( https://cge.cbs.dtu.dk/services/PlasmidFinder/ ) and MOB-suite v3.1.0 was used to identify plasmids and segregate plasmid-borne AMR genes 66 68 .…”
Section: Methodsmentioning
confidence: 99%
“…All Shigella isolates were enriched in Luria-Bertani broth at 37°C for 16 h, and genomic DNA was extracted using QIAamp DNA minikits (Qiagen) according to the manufacturer’s instructions. Whole-genome sequencing was carried out using Illumina technology at the Genomics Centre of the International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), and the sequences were processed and assembled using a previously described methodology ( 46 ). The obtained genome sequences have been published in GenBank under the BioProject accession numbers PRJNA693631 , PRJNA694802 , PRJNA698772 , PRJNA704496 , and PRJNA698078 and announced publicly ( 46 ).…”
Section: Methodsmentioning
confidence: 99%
“…Whole-genome sequencing was carried out using Illumina technology at the Genomics Centre of the International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), and the sequences were processed and assembled using a previously described methodology ( 46 ). The obtained genome sequences have been published in GenBank under the BioProject accession numbers PRJNA693631 , PRJNA694802 , PRJNA698772 , PRJNA704496 , and PRJNA698078 and announced publicly ( 46 ). Annotation of the genomes was performed by NCBI through the NCBI Prokaryotic Genome Annotation Pipeline v5.0 ( 47 ).…”
Section: Methodsmentioning
confidence: 99%
“…Selected C. jejuni isolates were confirmed by species-specific PCR. Genomic DNA was extracted using the Wizard genomic DNA purification kit (Promega) followed by quality and quantity check using a NanoDrop spectrophotometer (Thermo Scientific) and Qubit 2.0 fluorimeter (Life Technologies), respectively ( 5 ). The sequencing library was constructed using 1 ng of genomic DNA employing Illumina Nextera XT DNA library preparation kit.…”
Section: Announcementmentioning
confidence: 99%