Draft Genome Sequences of Seven 4-Formylaminooxyvinylglycine Producers Belonging to the Pseudomonas fluorescens Species Complex

Okrent, Rachel A.; Manning, Viola A.; Trippe, Kristin M.

doi:10.1128/genomea.00277-17

Cited by 5 publications

(5 citation statements)

References 15 publications

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“…Curiously, some rhizosphere-associated P. fluorescens produce the vinylglycine analog, FVG, a germination-arrest factor that has been shown to limit the germination of weedy grasses ( McPhail et al, 2010 ; Okrent et al, 2017b ). The biosynthetic cluster involved in FVG production by P. fluorescens WH6 has recently been described ( Okrent et al, 2017a ), yet, not much is understood about the biological significance of FVG in the bacterial interaction with the plant host. ET is a known inducer of seed germination ( Corbineau et al, 2014 ), so it is possible that FVG and MVG inhibit ACS and ET production that arrests the germination of the seeds of some plants, however, this remains to be conclusively proven.…”

Section: Counter Attack! Bacterial Modulation Of Plant Acc and Et Levmentioning

confidence: 99%

Ethylene and 1-Aminocyclopropane-1-carboxylate (ACC) in Plant–Bacterial Interactions

2018

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Ethylene and its precursor 1-aminocyclopropane-1-carboxylate (ACC) actively participate in plant developmental, defense and symbiotic programs. In this sense, ethylene and ACC play a central role in the regulation of bacterial colonization (rhizospheric, endophytic, and phyllospheric) by the modulation of plant immune responses and symbiotic programs, as well as by modulating several developmental processes, such as root elongation. Plant-associated bacterial communities impact plant growth and development, both negatively (pathogens) and positively (plant-growth promoting and symbiotic bacteria). Some members of the plant-associated bacterial community possess the ability to modulate plant ACC and ethylene levels and, subsequently, modify plant defense responses, symbiotic programs and overall plant development. In this work, we review and discuss the role of ethylene and ACC in several aspects of plant-bacterial interactions. Understanding the impact of ethylene and ACC in both the plant host and its associated bacterial community is key to the development of new strategies aimed at increased plant growth and protection.

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Section: Counter Attack! Bacterial Modulation Of Plant Acc and Et Levmentioning

confidence: 99%

Ethylene and 1-Aminocyclopropane-1-carboxylate (ACC) in Plant–Bacterial Interactions

2018

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“…The sequencing, assembly and annotation of the genomes is described in Okrent et al [ 43 ]. The contigs were ordered based on whole genome alignment with closely related complete genomes and the presence of the gvg gene cluster was confirmed.…”

Section: Methodsmentioning

confidence: 99%

Unexpected distribution of the 4-formylaminooxyvinylglycine (FVG) biosynthetic pathway in Pseudomonas and beyond

et al. 2021

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The biological herbicide and antibiotic 4-formylaminooxyvinylglycine (FVG) was originally isolated from several rhizosphere-associated strains of Pseudomonas fluorescens. Biosynthesis of FVG is dependent on the gvg biosynthetic gene cluster in P. fluorescens. In this investigation, we used comparative genomics to identify strains with the genetic potential to produce FVG due to presence of a gvg gene cluster. These strains primarily belong to two groups of Pseudomonas, P. fluorescens and P. syringae, however, a few strains with the gvg cluster were found outside of Pseudomonas. Mass spectrometry confirmed that all tested strains of the P. fluorescens species group produced FVG. However, P. syringae strains did not produce FVG under standard conditions. Several lines of evidence regarding the transmission of the gvg cluster including a robust phylogenetic analysis suggest that it was introduced multiple times through horizontal gene transfer within the Pseudomonas lineage as well as in select lineages of Thiomonas, Burkholderia and Pantoea. Together, these data broaden our understanding of the evolution and diversity of FVG biosynthesis. In the course of this investigation, additional gene clusters containing only a subset of the genes required to produce FVG were identified in a broad range of bacteria, including many non-pseudomonads.

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“…The copyright holder for this this version posted February 8, 2021. ; https://doi.org/10.1101/2021.02.08.430221 doi: bioRxiv preprint P. fluorescens P5A was performed by amplifying and sequencing 16S rDNA using standard protocols. The sequencing, assembly and annotation of the genomes is described in Okrent et al [39].…”

Section: Bacterial Isolationmentioning

confidence: 99%

“…Initial taxonomic identification of P. fluorescens P5A was performed by amplifying and sequencing 16S rDNA using standard protocols. The sequencing, assembly and annotation of the genomes is described in Okrent et al [39].…”

Section: Bacterial Isolationmentioning

confidence: 99%

Unexpected distribution of the 4-formylaminooxyvinylglycine (FVG) biosynthetic pathway inPseudomonasand beyond

Davis

Okrent

Manning

et al. 2021

Preprint

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The biological herbicide and antibiotic 4-formylaminooxyvinylglycine (FVG) was originally isolated from several rhizosphere-associated strains of Pseudomonas fluorescens . Biosynthesis of FVG is dependent on the gvg biosynthetic gene cluster in P. fluorescens . In this investigation, we used comparative genomics to identify strains with the genetic potential to produce FVG due to presence of a gvg gene cluster. These strains primarily belong to two groups of Pseudomonas , P. fluorescens and P. syringae , however, a few strains with the gvg cluster were found outside of Pseudomonas . Mass spectrometry confirmed that all tested strains of the P. fluorescens species group produced FVG. However, P. syringae strains did not produce FVG under standard conditions. Several lines of evidence regarding the transmission of the gvg cluster including a robust phylogenetic analysis suggest that it was introduced multiple times through horizontal gene transfer within the Pseudomonas lineage as well as in select lineages of Thiomonas , Burkholderia and Pantoea . Together, these data broaden our understanding of the evolution and diversity of FVG biosynthesis. In the course of this investigation, additional gene clusters containing only a subset of the genes required to produce FVG were identified in a broad range of bacteria, including many non-pseudomonads.

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Draft Genome Sequences of Seven 4-Formylaminooxyvinylglycine Producers Belonging to the Pseudomonas fluorescens Species Complex

Cited by 5 publications

References 15 publications

Ethylene and 1-Aminocyclopropane-1-carboxylate (ACC) in Plant–Bacterial Interactions

Ethylene and 1-Aminocyclopropane-1-carboxylate (ACC) in Plant–Bacterial Interactions

Unexpected distribution of the 4-formylaminooxyvinylglycine (FVG) biosynthetic pathway in Pseudomonas and beyond

Unexpected distribution of the 4-formylaminooxyvinylglycine (FVG) biosynthetic pathway inPseudomonasand beyond

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