Draft Genome Sequences of Six Strains of Lactococcus lactis (Phylum
 Firmicutes
 ), Spanning the Seeds of
 Cucumis sativus
 L. (Cucumber),
 Cucumis melo
 L. (Cantaloupe), and
 Cucurbita pepo
 var.
 turbinate
 (Acorn Squash)

Khalaf, Eman M.; Raizada, Manish N.

doi:10.1128/mra.00665-20

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…L. lactis isolates were cultivated from the aerial root mucilage of Sierra Mixe, a Mexican highland landrace of maize from Oaxaca, proximal to several landraces in this study; Sierra Mixe was shown to have significant nitrogen fixation activity ( Higdon et al, 2020 ). L. lactis isolates from cucurbit seeds were shown to secrete auxin and acetoin, solubilize phosphate and have antifungal activities ( Khalaf and Raizada, 2016 , 2018 , 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Khalaf,

Shrestha,

Reid

et al. 2023

Front. Microbiol.

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Pollen is a vector for diversification, fitness-selection, and transmission of plant genetic material. The extent to which the pollen microbiome may contribute to host diversification is largely unknown, because pollen microbiome diversity within a plant species has not been reported, and studies have been limited to conventional short-read 16S rRNA gene sequencing (e.g., V4-MiSeq) which suffers from poor taxonomic resolution. Here we report the pollen microbiomes of 16 primitive and traditional accessions of maize (corn) selected by indigenous peoples across the Americas, along with the modern U.S. inbred B73. The maize pollen microbiome has not previously been reported. The pollen microbiomes were identified using full-length (FL) 16S rRNA gene PacBio SMRT sequencing compared to V4-MiSeq. The Pan-American maize pollen microbiome encompasses 765 taxa spanning 39 genera and 46 species, including known plant growth promoters, insect-obligates, plant pathogens, nitrogen-fixers and biocontrol agents. Eleven genera and 13 species composed the core microbiome. Of 765 taxa, 63% belonged to only four genera: 28% were Pantoea, 15% were Lactococcus, 11% were Pseudomonas, and 10% were Erwinia. Interestingly, of the 215 Pantoea taxa, 180 belonged to a single species, P. ananatis. Surprisingly, the diversity within P. ananatis ranged nearly 10-fold amongst the maize accessions analyzed (those with ≥3 replicates), despite being grown in a common field. The highest diversity within P. ananatis occurred in accessions that originated near the center of diversity of domesticated maize, with reduced diversity associated with the north–south migration of maize. This sub-species diversity was revealed by FL-PacBio but missed by V4-MiSeq. V4-MiSeq also mis-identified some dominant genera captured by FL-PacBio. The study, though limited to a single season and common field, provides initial evidence that pollen microbiomes reflect evolutionary and migratory relationships of their host plants.

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Section: Discussionmentioning

confidence: 99%

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Khalaf,

Shrestha,

Reid

et al. 2023

Front. Microbiol.

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“…Probiotic species that can alter the gut microbiota are included in a variety of pharmacological regimens for the treatment of human and animal diseases. Probiotics are living commensal bacteria with anticarcinogenic and immune-modulating capabilities that boost human immunity (Fooladi et al, 2015;Khalaf & Raizada, 2020). Other lactic acid bacteria (LAB) can impact immune responses if fed in sufficient proportions (El-Baz et al, 2021;Fooladi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Hyphaena thebaica and Lactobacillus in Dextran Sodium Sulphate-Induced Ulcerative Colitis in Rats

Shata¹,

Shaaban²,

Abou-Hany³

et al. 2022

Delta University Scientific Journal

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Ulcerative colitis (UC) can cause a variety of complications, ranging from colon damage to colorectal cancer. The cause of UC, as well as successful treatment options, remain a mystery. The mechanisms behind the coloprotective benefits of Hyphaena thebaica (H. thebaica) and Lactobacillus, as well as their combined therapy on dextran sodium sulphate (DSS)-induced colitis in rats, are the subject of this study. The experimental panel included seven groups of rats; normal control, negative plus H. thebaica, negative plus Lactobacillus, UC diseased rats, H. thebaica treated, Lactobacillus treated, and combination of H. thebaica and Lactobacillus treated. Clinical evaluation of UC and both macroscopic and microscopic examination scoring was also done. Colonic oxidants/antioxidant stress biomarkers; malondialdehyde (MDA), glutathione (GSH), catalase, myeloperoxidase activity, and superoxide dismutase (SOD) activity were assessed. Colon Nrf2, HO-1 contents, TNF-α as well as caspase and Bax proteins was evaluated. H. thebaica in conjunction with Lactobacillus, greatly reduced dextran sodium sulphate-induced colon injury (DSS). In addition, the tested drugs dramatically decreased DSS-induced oxidative, inflammatory, and apoptotic activities.

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A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

Khalaf

Hassan

El-Baz

et al. 2022

European Journal of Pharmacology

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Draft Genome Sequences of Six Strains of Lactococcus lactis (Phylum Firmicutes ), Spanning the Seeds of Cucumis sativus L. (Cucumber), Cucumis melo L. (Cantaloupe), and Cucurbita pepo var. turbinate (Acorn Squash)

Cited by 4 publications

References 22 publications

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

The Effects of Hyphaena thebaica and Lactobacillus in Dextran Sodium Sulphate-Induced Ulcerative Colitis in Rats

A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

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