Draft Genome Sequences of Six Lactobacillus gasseri and Three
            <i>Lactobacillus paragasseri</i>
            Strains Isolated from the Female Bladder

Putonti, Catherine; Akhnoukh, Virina; Anagnostopoulos, Zoe; Bilek, M; Colgan, John J; Idrissi, Samir El; Magnuszewski, Karolina; Marino, Steven; Szymulewski, Matthew; Tyahla, Megan; Yanchuk, Viktoriya; Miller-Ensminger, Taylor; Batrich, Mary; Brassil, Bridget; Mormando, Rita; Johnson, Genevieve; Wolfe, Alan J.

doi:10.1128/mra.00973-19

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…gasseri groups we identified correspond to two previously defined groups with distinct gene content [51]. Notably, recent studies [52, 53] suggest our L. gasseri group 3 represents the closely related L.…”

Section: Discussionsupporting

confidence: 69%

“…The three L. gasseri groups we identified correspond to two previously defined groups with distinct gene content [ 51 ]. Notably, recent studies [ 52, 53 ] suggest our L. gasseri group 3 represents the closely related L. paragasseri . The three L. crispatus groups correlate with two previously reported groups described in a genomic analysis of 41 strains [ 4 ].…”

Section: Discussionmentioning

confidence: 79%

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The structure and diversity of strain-level variation in vaginal bacteria

2021

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The vaginal microbiome plays an important role in human health and species of vaginal bacteria have been associated with reproductive disease. Strain-level variation is also thought to be important, but the diversity, structure and evolutionary history of vaginal strains is not as well characterized. We developed and validated an approach to measure strain variation from metagenomic data based on SNPs within the core genomes for six species of vaginal bacteria: Gardnerella vaginalis , Lactobacillus crispatus , Lactobacillus iners , Lactobacillus jensenii , Lactobacillus gasseri and Atopobium vaginae . Despite inhabiting the same environment, strain diversity and structure varies across species. All species except L. iners are characterized by multiple distinct groups of strains. Even so, strain diversity is lower in the Lactobacillus species, consistent with a more recent colonization of the human vaginal microbiome. Both strain diversity and the frequency of multi-strain samples is related to species-level diversity of the microbiome in which they occur, suggesting similar ecological factors influencing diversity within the vaginal niche. We conclude that the structure of strain-level variation provides both the motivation and means of testing whether strain-level differences contribute to the function and health consequences of the vaginal microbiome.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 79%

The structure and diversity of strain-level variation in vaginal bacteria

2021

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“…The three L. gasseri groups we identified correspond to two previously defined groups with distinct gene content (52). Notably, recent studies (53,54) suggest our L. gasseri Group 3 represents the closely related L. paragasseri. The three L. crispatus groups correlate with two previously reported groups described in a genomic analysis of 41 strains (4).…”

Section: Strain Level Variationsupporting

confidence: 66%

The structure and diversity of strain level variation in vaginal bacteria

Tortelli

Lewis

Fay

2020

Preprint

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The vaginal microbiome plays an important role in human health and species of vaginal bacteria have been associated with reproductive disease. Strain level variation is also thought to be important, but the diversity, structure and evolutionary history of vaginal strains is not as well characterized. We developed and validated an approach to measure strain variation from metagenomic data based on SNPs within the core-genomes for six species of vaginal bacteria: G. vaginalis, L. crispatus, L. iners, L. jensenii, L. gasseri, and A. vaginae. Despite inhabiting the same environment, strain diversity and structure varies across species. All species except L. iners are characterized by multiple distinct groups of strains. Even so, strain diversity is lower in the Lactobacillus species, consistent with a more recent colonization of the human vaginal microbiome. Both strain diversity and the frequency of multi-strain samples is related to species-level diversity of the microbiome in which they occur, suggesting similar ecological factors influencing diversity within the vaginal niche. We conclude that the structure of strain level variation provides both the motivation and means of testing whether strain level differences contribute to the function and health consequences of the vaginal microbiome.Data SummaryAll vaginal metagenomic sequence data generated for this project can be found on the Sequence Read Archive under BioProject PRJNA639592.

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“…Partial genomic sequences were available at NCBI ( https://www.ncbi.nlm.nih.gov/genome/ ) for all three strains. Updated taxonomy was validated by Genome Taxonomy Database ( https://gtdb.ecogenomic.org/ ), which is the most up-to-date taxonomic lineage for L. paragasseri JV-V03, formerly L. gasseri ( 79 , 80 , 121 ), and L. mulieris JV-V16, formerly L. jensenii ( 81 , 82 ).…”

Section: Methodsmentioning

confidence: 99%