Draft Genome Sequences of Two
            <i>Aspergillus fumigatus</i>
            Strains, Isolated from the International Space Station

Singh, Nitin Kumar; Blachowicz, Adriana; Checinska, Aleksandra; Wang, Clay; Venkateswaran, Kasthuri

doi:10.1128/genomea.00553-16

Cited by 18 publications

(10 citation statements)

References 6 publications

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“…Whole-genome sequencing (WGS) of ISSFT-021 and IF1SW-F4 (26) reveal 54,960 and 52,129 single nucleotide polymorphisms (SNPs) compared to the clinical isolate and model laboratory strain Af293, respectively, which is not outside the genetic diversity observed among 95 sequenced A. fumigatus isolates (Fig. 1B).…”

Section: Resultsmentioning

confidence: 98%

“…Following initial culture and ITS sequence-based identification of the isolates as A. fumigatus , we undertook WGS to facilitate the current and future studies of ISSFT-021 and IF1SW-F4 (26). Analysis of SNPs among all publicly available sequenced isolates (>100 total; 95 unique isolates included in the present study) across a global range of clinical and environmental sources shows considerable genetic diversity, consistent with previous reports of genetic variance in A. fumigatus (22).…”

Section: Discussionmentioning

confidence: 99%

“…Future studies aimed at guiding enhanced disinfectant protocols aboard space vessels should consider these data and pursue effective methods for clearing surfaces of A. fumigatus . However, it was observed that both ISSFT-021 and IF1SW-F4 were significantly more resistant to UV irradiation than clinical isolates (26), and current studies are under way that will leverage sequence data and molecular approaches to elucidate these mechanisms. It is critical to note that care must be taken in interpreting these results, as terrestrial equivalents or reference strains, even if identified as the same species, do not represent experimental controls.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Aspergillus fumigatus Isolates from Air and Surfaces of the International Space Station

Knox

Blachowicz

Palmer

et al. 2016

mSphere

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As durations of manned space missions increase, it is imperative to understand the long-term consequence of microbial exposure on human health in a closed human habitat. To date, studies aimed at bacterial and fungal contamination of space vessels have highlighted species compositions biased toward hardy, persistent organisms capable of withstanding harsh conditions. In the current study, we assessed traits of two independent Aspergillus fumigatus strains isolated from the International Space Station. Ubiquitously found in terrestrial soil and atmospheric environments, A. fumigatus is a significant opportunistic fungal threat to human health, particularly among the immunocompromised. Using two well-known clinical isolates of A. fumigatus as comparators, we found that both ISS isolates exhibited normal in vitro growth and chemical stress tolerance yet caused higher lethality in a vertebrate model of invasive disease. These findings substantiate the need for additional studies of physical traits and biological activities of microbes adapted to microgravity and other extreme extraterrestrial conditions.

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of Aspergillus fumigatus Isolates from Air and Surfaces of the International Space Station

Knox

Blachowicz

Palmer

et al. 2016

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“…Fungi are important sources of natural products that are useful to humankind. Bioactive components generated by fungi have been used in pharmaceutical, food industries and agriculture ,,. Natural products obtained from fungi are generally cultivated under standardized laboratory growth conditions .…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Manipulation Induces the Production of Coumarin‐Type Secondary Metabolite from Arthrobotrys foliicola

Hsueh

et al. 2019

Israel Journal of Chemistry

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Pursuing epigenetic manipulation approach in fungi led to the isolation of an unusual coumarin metabolite from fungi. An addition of the histone deacetylase inhibitor, suberohydroxamic acid (SBHA), to the culture medium of Arthrobotrys foliicola induced production of the coumarin‐type secondary metabolite represented by a single intensive peak in the HPLC profile of the ethyl acetate extract. The compound which was identified as 4‐ethyl‐7‐hydroxy‐8‐methyl‐2H‐chromen‐2‐one (1) was isolated from nature for the first time. Moreover, the investigation on the remaining part of the HPLC profile led to the separation of 6‐ethyl‐2,4‐dihydroxy‐3‐methylbenzaldehyde (2) and ten 2,5‐diketopiperazine compounds (3–12). The structures of isolates were deduced by their mass and NMR spectroscopic data. The coumarin‐type secondary metabolite (1) with peculiar smell induced by epigenetic stimulation is found for the first time in the Arthrobotrys species and the family Orbiliaceae. We evaluated 1 for the cytotoxic, anti‐inflammatory, anti‐allergic and nematocidal activities, however, it was found inactive.

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“…The complete genomes are available for all of these strains. 35,36 • Sponge dataset (SPONGE). This dataset contains 223,135 spectra from bacterial extracts of Theonella swinhoei (GNPS dataset MSV000078670).…”

mentioning

confidence: 99%

MetaRiPPquest: A Peptidogenomics Approach for the Discovery of Ribosomally Synthesized and Post-translationally Modified Peptides

Mohimani

Gurevich

et al. 2017

Preprint

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Ribosomally synthesized and post-translationally modified peptides (RiPPs) are an important class of natural products that include many antibiotics and a variety of other bioactive compounds. While recent breakthroughs in RiPP discovery raised the challenge of developing new algorithms for their analysis, peptidogenomic-based identification of RiPPs by combining genome/metagenome mining with analysis of tandem mass spectra remains an open problem. We present here MetaRiPPquest, a software tool for addressing this challenge that is compatible with large-scale screening platforms for natural product discovery. After searching millions of spectra in the Global Natural Products Social (GNPS) molecular networking infrastructure against just six genomic and metagenomic datasets, MetaRiPPquest identified 27 known and discovered 5 novel RiPP natural products. 1 spectra of natural products. However, to transform natural product discovery into a high-throughput technology and to fully realize the promise of the GNPS project, new algorithms are needed for natural products discovery 6-10 . Indeed, while spectra in the GNPS molecular network represent a gold mine for future chemical discoveries, their interpretation remains a bottleneck due to the large volume of data produced by modern mass spectrometers and unavailability of computational platforms for data processing.The efforts present herein focus on Ribosomally synthesized and Post-translationally modified Peptides (RiPPs), a rapidly expanding group of natural products with applications in pharmaceutical and food industries 11 . RiPPs are produced by RiPP Synthetases (RiPPS) through the Post Ribosomal Peptide Synthesis (PRPS) pathway 11 . RiPPs are initially synthesized as precursor peptides, encoded by RiPP structural genes. The RiPP structural genes are often quite short, making their annotation difficult 12 . A precursor peptide consists of a prefix leader peptide appended to a suffix core peptide. A leader peptide is important for recognition by the RiPP post-translational modification enzymes and for exporting the RiPP out of the cell. The core peptide is post-translationally modified by the RiPP biosynthetic machinery, proteolytically cleaved from the leader peptide to yield the mature RiPP, and exported out of the cell by transporters. The precursor peptide and the enzymes responsible for post-translational modifications (PTMs), proteolytic cleavage, and transportation usually appear in a contiguous biosynthetic gene cluster (BGC) of a RiPP within a microbial genome. The length of the microbial RiPP-encoding BGCs typically varies from 1,000 to 40,000 bp (average length 10,000 bp), larger than the current length of short reads generated by next generation sequencing (350bp), and making DNA assembly a critical part of any short read based RiPP discovery method.Genome mining refers to the informatics-based structural interpretation of a natural product BGC to infer information about the natural product itself. The discoveries of coelichelin in Streptomyces coelicolo...

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Draft Genome Sequences of Two Aspergillus fumigatus Strains, Isolated from the International Space Station

Cited by 18 publications

References 6 publications

Characterization of Aspergillus fumigatus Isolates from Air and Surfaces of the International Space Station

Characterization of Aspergillus fumigatus Isolates from Air and Surfaces of the International Space Station

Epigenetic Manipulation Induces the Production of Coumarin‐Type Secondary Metabolite from Arthrobotrys foliicola

MetaRiPPquest: A Peptidogenomics Approach for the Discovery of Ribosomally Synthesized and Post-translationally Modified Peptides

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